Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3053 and GFF3054 are separated by 17 nucleotides GFF3054 and GFF3055 are separated by 18 nucleotides GFF3055 and GFF3056 overlap by 4 nucleotides GFF3056 and GFF3057 are separated by 7 nucleotides
GFF3053 - Twitching motility protein PilT, at 65,081 to 66,061
GFF3053
GFF3054 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 66,079 to 66,783
GFF3054
GFF3055 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 66,802 to 67,368
GFF3055
GFF3056 - COG1872, at 67,365 to 67,655
GFF3056
GFF3057 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 67,663 to 68,256
GFF3057
Position (kb) 66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 65.226 kb on - strand, within GFF3053 at 65.280 kb on - strand, within GFF3053 at 65.345 kb on - strand, within GFF3053 at 65.574 kb on + strand, within GFF3053 at 65.581 kb on - strand, within GFF3053 at 65.587 kb on + strand, within GFF3053 at 65.630 kb on - strand, within GFF3053 at 65.726 kb on + strand, within GFF3053 at 66.022 kb on + strand at 66.022 kb on + strand at 66.055 kb on + strand at 66.055 kb on + strand at 66.056 kb on - strand at 66.061 kb on + strand at 66.063 kb on + strand at 66.063 kb on + strand at 66.106 kb on + strand at 66.166 kb on - strand, within GFF3054 at 66.250 kb on + strand, within GFF3054 at 66.381 kb on + strand, within GFF3054 at 66.382 kb on - strand, within GFF3054 at 66.446 kb on + strand, within GFF3054 at 66.447 kb on - strand, within GFF3054 at 66.596 kb on - strand, within GFF3054 at 66.599 kb on + strand, within GFF3054 at 66.632 kb on + strand, within GFF3054 at 66.677 kb on + strand, within GFF3054 at 66.678 kb on - strand, within GFF3054 at 66.687 kb on + strand, within GFF3054 at 66.723 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.846 kb on + strand at 66.857 kb on + strand at 67.017 kb on + strand, within GFF3055 at 67.089 kb on + strand, within GFF3055 at 67.089 kb on + strand, within GFF3055 at 67.098 kb on + strand, within GFF3055 at 67.183 kb on + strand, within GFF3055 at 67.183 kb on + strand, within GFF3055 at 67.184 kb on - strand, within GFF3055 at 67.187 kb on + strand, within GFF3055 at 67.187 kb on + strand, within GFF3055 at 67.188 kb on - strand, within GFF3055 at 67.288 kb on + strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.300 kb on - strand, within GFF3055 at 67.319 kb on + strand at 67.319 kb on + strand at 67.439 kb on + strand, within GFF3056 at 67.653 kb on + strand at 67.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 65,226 - GFF3053 0.15 -0.7 65,280 - GFF3053 0.20 +0.3 65,345 - GFF3053 0.27 +0.1 65,574 + GFF3053 0.50 -0.4 65,581 - GFF3053 0.51 -0.5 65,587 + GFF3053 0.52 -1.1 65,630 - GFF3053 0.56 -0.1 65,726 + GFF3053 0.66 +0.4 66,022 + -0.7 66,022 + +0.1 66,055 + +0.3 66,055 + +0.2 66,056 - -0.5 66,061 + -0.0 66,063 + -0.1 66,063 + -0.3 66,106 + -0.5 66,166 - GFF3054 0.12 -1.6 66,250 + GFF3054 0.24 -1.3 66,381 + GFF3054 0.43 +0.3 66,382 - GFF3054 0.43 -1.4 66,446 + GFF3054 0.52 -0.6 66,447 - GFF3054 0.52 -1.2 66,596 - GFF3054 0.73 -0.2 66,599 + GFF3054 0.74 -1.2 66,632 + GFF3054 0.78 -0.9 66,677 + GFF3054 0.85 -1.3 66,678 - GFF3054 0.85 -0.5 66,687 + GFF3054 0.86 -1.1 66,723 + -1.9 66,844 + +0.4 66,844 + -2.3 66,844 + +0.2 66,846 + -0.2 66,857 + -0.9 67,017 + GFF3055 0.38 -0.5 67,089 + GFF3055 0.51 -0.3 67,089 + GFF3055 0.51 +0.5 67,098 + GFF3055 0.52 -0.4 67,183 + GFF3055 0.67 -0.1 67,183 + GFF3055 0.67 +0.4 67,184 - GFF3055 0.67 -0.7 67,187 + GFF3055 0.68 -0.4 67,187 + GFF3055 0.68 -0.2 67,188 - GFF3055 0.68 +0.5 67,288 + GFF3055 0.86 +0.4 67,289 - GFF3055 0.86 -0.6 67,289 - GFF3055 0.86 +0.5 67,300 - GFF3055 0.88 +1.3 67,319 + -0.9 67,319 + +0.7 67,439 + GFF3056 0.25 +0.5 67,653 + +1.8 67,657 + +0.5
Or see this region's nucleotide sequence