Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3000 and GFF3001 overlap by 2 nucleotides GFF3001 and GFF3002 are separated by 13 nucleotides GFF3002 and GFF3003 are separated by 225 nucleotides GFF3003 and GFF3004 are separated by 162 nucleotides GFF3004 and GFF3005 are separated by 25 nucleotides
GFF3000 - Putative uncharacterized protein STY3208 (Putative uncharacterized protein), at 15,922 to 16,656
GFF3000
GFF3001 - hypothetical protein, at 16,655 to 16,885
GFF3001
GFF3002 - hypothetical protein, at 16,899 to 17,045
GFF3002
GFF3003 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 17,271 to 20,144
GFF3003
GFF3004 - Glycine cleavage system H protein, at 20,307 to 20,696
GFF3004
GFF3005 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 20,722 to 21,816
GFF3005
Position (kb) 17
18
19
20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 16.345 kb on - strand, within GFF3000 at 16.362 kb on + strand, within GFF3000 at 16.384 kb on + strand, within GFF3000 at 16.402 kb on + strand, within GFF3000 at 16.522 kb on - strand, within GFF3000 at 16.523 kb on + strand, within GFF3000 at 17.267 kb on + strand at 17.272 kb on + strand at 17.357 kb on - strand at 17.552 kb on + strand at 17.553 kb on - strand at 17.699 kb on + strand, within GFF3003 at 17.699 kb on + strand, within GFF3003 at 17.734 kb on + strand, within GFF3003 at 17.734 kb on + strand, within GFF3003 at 17.734 kb on + strand, within GFF3003 at 17.773 kb on + strand, within GFF3003 at 17.934 kb on - strand, within GFF3003 at 18.023 kb on + strand, within GFF3003 at 18.187 kb on + strand, within GFF3003 at 18.269 kb on + strand, within GFF3003 at 18.388 kb on + strand, within GFF3003 at 18.415 kb on + strand, within GFF3003 at 18.437 kb on + strand, within GFF3003 at 18.498 kb on - strand, within GFF3003 at 18.671 kb on + strand, within GFF3003 at 18.671 kb on + strand, within GFF3003 at 18.672 kb on - strand, within GFF3003 at 18.674 kb on - strand, within GFF3003 at 18.674 kb on - strand, within GFF3003 at 19.094 kb on - strand, within GFF3003 at 19.486 kb on - strand, within GFF3003 at 19.494 kb on + strand, within GFF3003 at 19.810 kb on + strand, within GFF3003 at 19.810 kb on + strand, within GFF3003 at 19.819 kb on + strand, within GFF3003 at 19.879 kb on + strand at 19.879 kb on + strand at 19.894 kb on + strand at 19.895 kb on - strand at 19.913 kb on + strand at 20.624 kb on - strand, within GFF3004 at 20.624 kb on - strand, within GFF3004 at 20.624 kb on - strand, within GFF3004 at 20.643 kb on - strand, within GFF3004 at 20.702 kb on - strand at 20.849 kb on + strand, within GFF3005 at 20.938 kb on - strand, within GFF3005 at 20.938 kb on - strand, within GFF3005 at 21.025 kb on - strand, within GFF3005 at 21.087 kb on + strand, within GFF3005 at 21.088 kb on - strand, within GFF3005
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 16,345 - GFF3000 0.58 +0.3 16,362 + GFF3000 0.60 -0.0 16,384 + GFF3000 0.63 +0.7 16,402 + GFF3000 0.65 -1.3 16,522 - GFF3000 0.82 -0.7 16,523 + GFF3000 0.82 -0.2 17,267 + -0.1 17,272 + -0.3 17,357 - -0.1 17,552 + -0.2 17,553 - +0.1 17,699 + GFF3003 0.15 +0.3 17,699 + GFF3003 0.15 +0.8 17,734 + GFF3003 0.16 +0.9 17,734 + GFF3003 0.16 -3.1 17,734 + GFF3003 0.16 +1.6 17,773 + GFF3003 0.17 +0.1 17,934 - GFF3003 0.23 +1.1 18,023 + GFF3003 0.26 -0.6 18,187 + GFF3003 0.32 +0.8 18,269 + GFF3003 0.35 -0.1 18,388 + GFF3003 0.39 -0.0 18,415 + GFF3003 0.40 +0.3 18,437 + GFF3003 0.41 -0.3 18,498 - GFF3003 0.43 -0.3 18,671 + GFF3003 0.49 +1.3 18,671 + GFF3003 0.49 -0.1 18,672 - GFF3003 0.49 -0.7 18,674 - GFF3003 0.49 +0.1 18,674 - GFF3003 0.49 +0.1 19,094 - GFF3003 0.63 -0.3 19,486 - GFF3003 0.77 -0.8 19,494 + GFF3003 0.77 +0.2 19,810 + GFF3003 0.88 -0.1 19,810 + GFF3003 0.88 +0.9 19,819 + GFF3003 0.89 +1.1 19,879 + -0.3 19,879 + +0.5 19,894 + +0.7 19,895 - +0.2 19,913 + +1.1 20,624 - GFF3004 0.81 +0.4 20,624 - GFF3004 0.81 +0.0 20,624 - GFF3004 0.81 +0.6 20,643 - GFF3004 0.86 +0.2 20,702 - +0.5 20,849 + GFF3005 0.12 +0.3 20,938 - GFF3005 0.20 -0.7 20,938 - GFF3005 0.20 +0.6 21,025 - GFF3005 0.28 -0.0 21,087 + GFF3005 0.33 +0.2 21,088 - GFF3005 0.33 +1.2
Or see this region's nucleotide sequence