Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2997 and GFF2998 are separated by 163 nucleotides GFF2998 and GFF2999 are separated by 158 nucleotides GFF2999 and GFF3000 are separated by 47 nucleotides GFF3000 and GFF3001 overlap by 2 nucleotides
GFF2997 - COG1272: Predicted membrane protein hemolysin III homolog, at 13,148 to 13,807
GFF2997
GFF2998 - RNA-binding domain protein, at 13,971 to 14,282
GFF2998
GFF2999 - 6-phospho-beta-glucosidase (EC 3.2.1.86), at 14,441 to 15,874
GFF2999
GFF3000 - Putative uncharacterized protein STY3208 (Putative uncharacterized protein), at 15,922 to 16,656
GFF3000
GFF3001 - hypothetical protein, at 16,655 to 16,885
GFF3001
Position (kb) 14
15
16 Strain fitness (log2 ratio) -1
0
1
2 at 13.464 kb on - strand, within GFF2997 at 13.512 kb on + strand, within GFF2997 at 13.512 kb on + strand, within GFF2997 at 13.513 kb on - strand, within GFF2997 at 13.513 kb on - strand, within GFF2997 at 13.513 kb on - strand, within GFF2997 at 13.724 kb on + strand, within GFF2997 at 13.744 kb on + strand at 13.747 kb on + strand at 13.807 kb on + strand at 13.807 kb on + strand at 13.808 kb on - strand at 13.812 kb on + strand at 13.813 kb on - strand at 13.895 kb on - strand at 14.057 kb on + strand, within GFF2998 at 14.166 kb on + strand, within GFF2998 at 14.167 kb on - strand, within GFF2998 at 14.211 kb on - strand, within GFF2998 at 14.322 kb on + strand at 14.382 kb on - strand at 14.382 kb on - strand at 14.422 kb on - strand at 14.518 kb on + strand at 14.612 kb on + strand, within GFF2999 at 14.860 kb on - strand, within GFF2999 at 14.944 kb on + strand, within GFF2999 at 14.945 kb on - strand, within GFF2999 at 14.945 kb on - strand, within GFF2999 at 14.955 kb on - strand, within GFF2999 at 15.035 kb on + strand, within GFF2999 at 15.068 kb on + strand, within GFF2999 at 15.069 kb on - strand, within GFF2999 at 15.078 kb on + strand, within GFF2999 at 15.078 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.177 kb on - strand, within GFF2999 at 15.190 kb on + strand, within GFF2999 at 15.245 kb on - strand, within GFF2999 at 15.278 kb on + strand, within GFF2999 at 15.365 kb on + strand, within GFF2999 at 15.383 kb on + strand, within GFF2999 at 15.462 kb on - strand, within GFF2999 at 15.466 kb on + strand, within GFF2999 at 15.531 kb on - strand, within GFF2999 at 15.540 kb on - strand, within GFF2999 at 15.556 kb on + strand, within GFF2999 at 15.592 kb on - strand, within GFF2999 at 15.746 kb on + strand at 15.771 kb on - strand at 15.771 kb on - strand at 15.771 kb on - strand at 15.873 kb on - strand at 15.938 kb on - strand at 16.050 kb on + strand, within GFF3000 at 16.095 kb on + strand, within GFF3000 at 16.095 kb on + strand, within GFF3000 at 16.096 kb on - strand, within GFF3000 at 16.110 kb on + strand, within GFF3000 at 16.137 kb on + strand, within GFF3000 at 16.189 kb on - strand, within GFF3000 at 16.345 kb on - strand, within GFF3000 at 16.362 kb on + strand, within GFF3000 at 16.384 kb on + strand, within GFF3000 at 16.402 kb on + strand, within GFF3000 at 16.522 kb on - strand, within GFF3000 at 16.523 kb on + strand, within GFF3000
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 13,464 - GFF2997 0.48 -0.8 13,512 + GFF2997 0.55 -0.5 13,512 + GFF2997 0.55 -0.5 13,513 - GFF2997 0.55 +0.0 13,513 - GFF2997 0.55 +0.0 13,513 - GFF2997 0.55 -0.3 13,724 + GFF2997 0.87 +0.0 13,744 + -0.2 13,747 + -0.2 13,807 + +0.8 13,807 + +0.0 13,808 - +0.3 13,812 + +0.3 13,813 - +0.4 13,895 - +1.9 14,057 + GFF2998 0.28 -0.9 14,166 + GFF2998 0.62 -0.3 14,167 - GFF2998 0.63 -0.5 14,211 - GFF2998 0.77 +0.6 14,322 + -0.7 14,382 - -0.0 14,382 - -0.1 14,422 - -0.3 14,518 + -0.9 14,612 + GFF2999 0.12 +0.4 14,860 - GFF2999 0.29 -1.7 14,944 + GFF2999 0.35 -0.2 14,945 - GFF2999 0.35 -0.1 14,945 - GFF2999 0.35 -0.1 14,955 - GFF2999 0.36 -0.1 15,035 + GFF2999 0.41 -0.7 15,068 + GFF2999 0.44 -0.5 15,069 - GFF2999 0.44 -1.0 15,078 + GFF2999 0.44 -0.6 15,078 + GFF2999 0.44 -0.5 15,154 + GFF2999 0.50 -0.7 15,154 + GFF2999 0.50 -0.2 15,154 + GFF2999 0.50 -0.6 15,154 + GFF2999 0.50 -0.2 15,177 - GFF2999 0.51 -1.0 15,190 + GFF2999 0.52 +0.3 15,245 - GFF2999 0.56 -0.5 15,278 + GFF2999 0.58 -1.3 15,365 + GFF2999 0.64 +0.1 15,383 + GFF2999 0.66 -1.0 15,462 - GFF2999 0.71 +1.3 15,466 + GFF2999 0.71 -0.4 15,531 - GFF2999 0.76 +0.9 15,540 - GFF2999 0.77 -0.0 15,556 + GFF2999 0.78 +1.8 15,592 - GFF2999 0.80 -0.5 15,746 + -0.9 15,771 - -0.6 15,771 - +0.1 15,771 - +0.7 15,873 - -0.2 15,938 - -0.3 16,050 + GFF3000 0.17 +0.5 16,095 + GFF3000 0.24 -0.6 16,095 + GFF3000 0.24 +1.1 16,096 - GFF3000 0.24 +0.3 16,110 + GFF3000 0.26 -0.1 16,137 + GFF3000 0.29 -0.7 16,189 - GFF3000 0.36 +0.0 16,345 - GFF3000 0.58 +0.3 16,362 + GFF3000 0.60 -0.0 16,384 + GFF3000 0.63 +0.7 16,402 + GFF3000 0.65 -1.3 16,522 - GFF3000 0.82 -0.7 16,523 + GFF3000 0.82 -0.2
Or see this region's nucleotide sequence