Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF289 and GFF290 overlap by 11 nucleotides GFF290 and GFF291 overlap by 4 nucleotides GFF291 and GFF292 are separated by 2 nucleotides GFF292 and GFF293 overlap by 35 nucleotides GFF293 and GFF294 are separated by 17 nucleotides
GFF289 - Putative inner membrane protein, at 5,995 to 6,342
GFF289
GFF290 - Putative inner membrane protein, at 6,332 to 6,694
GFF290
GFF291 - Putative arylsulfatase regulatory protein, at 6,691 to 7,221
GFF291
GFF292 - hypothetical protein, at 7,224 to 7,337
GFF292
GFF293 - D-serine dehydratase (EC 4.3.1.18), at 7,303 to 8,625
GFF293
GFF294 - D-serine permease DsdX, at 8,643 to 9,980
GFF294
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-1
0
1
2 at 6.311 kb on + strand at 6.347 kb on - strand at 6.388 kb on + strand, within GFF290 at 6.389 kb on - strand, within GFF290 at 6.422 kb on + strand, within GFF290 at 6.532 kb on + strand, within GFF290 at 6.688 kb on + strand at 6.789 kb on - strand, within GFF291 at 6.789 kb on - strand, within GFF291 at 6.820 kb on - strand, within GFF291 at 6.900 kb on + strand, within GFF291 at 6.905 kb on + strand, within GFF291 at 6.905 kb on + strand, within GFF291 at 6.912 kb on + strand, within GFF291 at 6.913 kb on - strand, within GFF291 at 6.920 kb on + strand, within GFF291 at 6.930 kb on + strand, within GFF291 at 6.939 kb on + strand, within GFF291 at 7.049 kb on + strand, within GFF291 at 7.050 kb on - strand, within GFF291 at 7.134 kb on + strand, within GFF291 at 7.139 kb on - strand, within GFF291 at 7.152 kb on + strand, within GFF291 at 7.304 kb on + strand at 7.526 kb on + strand, within GFF293 at 7.526 kb on + strand, within GFF293 at 7.526 kb on + strand, within GFF293 at 7.526 kb on + strand, within GFF293 at 7.527 kb on - strand, within GFF293 at 7.527 kb on - strand, within GFF293 at 7.527 kb on - strand, within GFF293 at 7.551 kb on - strand, within GFF293 at 7.606 kb on + strand, within GFF293 at 7.681 kb on - strand, within GFF293 at 7.960 kb on - strand, within GFF293 at 7.997 kb on + strand, within GFF293 at 8.003 kb on - strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.068 kb on + strand, within GFF293 at 8.068 kb on + strand, within GFF293 at 8.069 kb on - strand, within GFF293 at 8.069 kb on - strand, within GFF293 at 8.080 kb on - strand, within GFF293 at 8.104 kb on - strand, within GFF293 at 8.193 kb on + strand, within GFF293 at 8.193 kb on + strand, within GFF293 at 8.194 kb on - strand, within GFF293 at 8.196 kb on - strand, within GFF293 at 8.261 kb on + strand, within GFF293 at 8.261 kb on + strand, within GFF293 at 8.262 kb on - strand, within GFF293 at 8.486 kb on - strand, within GFF293 at 8.511 kb on - strand at 8.628 kb on + strand at 8.629 kb on - strand at 8.638 kb on + strand at 8.638 kb on + strand at 8.652 kb on - strand at 8.963 kb on + strand, within GFF294 at 8.963 kb on + strand, within GFF294 at 8.963 kb on + strand, within GFF294 at 8.963 kb on + strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.970 kb on - strand, within GFF294 at 8.970 kb on - strand, within GFF294 at 8.970 kb on - strand, within GFF294 at 9.095 kb on + strand, within GFF294 at 9.099 kb on + strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.116 kb on + strand, within GFF294 at 9.135 kb on + strand, within GFF294 at 9.136 kb on - strand, within GFF294 at 9.140 kb on - strand, within GFF294 at 9.140 kb on - strand, within GFF294 at 9.140 kb on - strand, within GFF294 at 9.203 kb on + strand, within GFF294 at 9.205 kb on + strand, within GFF294 at 9.205 kb on + strand, within GFF294 at 9.387 kb on + strand, within GFF294 at 9.432 kb on + strand, within GFF294 at 9.433 kb on - strand, within GFF294 at 9.439 kb on + strand, within GFF294 at 9.581 kb on + strand, within GFF294
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 6,311 + -0.6 6,347 - -0.1 6,388 + GFF290 0.15 -0.3 6,389 - GFF290 0.16 +0.0 6,422 + GFF290 0.25 +0.1 6,532 + GFF290 0.55 +0.2 6,688 + -0.7 6,789 - GFF291 0.18 -0.1 6,789 - GFF291 0.18 +0.5 6,820 - GFF291 0.24 -0.2 6,900 + GFF291 0.39 +0.5 6,905 + GFF291 0.40 -0.1 6,905 + GFF291 0.40 -0.1 6,912 + GFF291 0.42 +0.1 6,913 - GFF291 0.42 +0.2 6,920 + GFF291 0.43 +0.0 6,930 + GFF291 0.45 +0.2 6,939 + GFF291 0.47 +0.3 7,049 + GFF291 0.67 -0.5 7,050 - GFF291 0.68 +1.4 7,134 + GFF291 0.83 -0.2 7,139 - GFF291 0.84 -0.4 7,152 + GFF291 0.87 +0.6 7,304 + +0.1 7,526 + GFF293 0.17 +0.7 7,526 + GFF293 0.17 +0.5 7,526 + GFF293 0.17 +0.8 7,526 + GFF293 0.17 +0.6 7,527 - GFF293 0.17 +0.0 7,527 - GFF293 0.17 +0.5 7,527 - GFF293 0.17 -0.3 7,551 - GFF293 0.19 +0.1 7,606 + GFF293 0.23 +0.3 7,681 - GFF293 0.29 +0.3 7,960 - GFF293 0.50 +1.1 7,997 + GFF293 0.52 +0.3 8,003 - GFF293 0.53 +0.2 8,064 + GFF293 0.58 -0.1 8,064 + GFF293 0.58 +0.4 8,064 + GFF293 0.58 -0.4 8,064 + GFF293 0.58 -0.6 8,068 + GFF293 0.58 +0.3 8,068 + GFF293 0.58 +0.3 8,069 - GFF293 0.58 +0.3 8,069 - GFF293 0.58 +0.3 8,080 - GFF293 0.59 -0.1 8,104 - GFF293 0.61 +1.0 8,193 + GFF293 0.67 -0.0 8,193 + GFF293 0.67 -1.7 8,194 - GFF293 0.67 +0.3 8,196 - GFF293 0.67 +0.0 8,261 + GFF293 0.72 +0.3 8,261 + GFF293 0.72 +0.1 8,262 - GFF293 0.72 +0.5 8,486 - GFF293 0.89 -0.0 8,511 - +1.2 8,628 + +0.6 8,629 - -0.1 8,638 + -0.2 8,638 + -0.0 8,652 - -0.7 8,963 + GFF294 0.24 -0.3 8,963 + GFF294 0.24 -0.2 8,963 + GFF294 0.24 +0.2 8,963 + GFF294 0.24 -0.1 8,964 - GFF294 0.24 +0.5 8,964 - GFF294 0.24 -0.1 8,964 - GFF294 0.24 +0.1 8,964 - GFF294 0.24 -0.3 8,970 - GFF294 0.24 -0.1 8,970 - GFF294 0.24 +0.4 8,970 - GFF294 0.24 +0.3 9,095 + GFF294 0.34 -0.2 9,099 + GFF294 0.34 +0.7 9,100 - GFF294 0.34 -1.6 9,100 - GFF294 0.34 -1.6 9,100 - GFF294 0.34 +0.3 9,100 - GFF294 0.34 -0.7 9,116 + GFF294 0.35 -0.1 9,135 + GFF294 0.37 -0.1 9,136 - GFF294 0.37 +0.3 9,140 - GFF294 0.37 +0.2 9,140 - GFF294 0.37 +0.6 9,140 - GFF294 0.37 -0.1 9,203 + GFF294 0.42 +1.6 9,205 + GFF294 0.42 +1.9 9,205 + GFF294 0.42 +0.3 9,387 + GFF294 0.56 -0.1 9,432 + GFF294 0.59 +0.8 9,433 - GFF294 0.59 +0.1 9,439 + GFF294 0.59 -1.3 9,581 + GFF294 0.70 -0.3
Or see this region's nucleotide sequence