Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2926 and GFF2927 are separated by 15 nucleotides GFF2927 and GFF2928 are separated by 5 nucleotides GFF2928 and GFF2929 are separated by 129 nucleotides GFF2929 and GFF2930 are separated by 19 nucleotides GFF2930 and GFF2931 are separated by 116 nucleotides
GFF2926 - GTP-binding protein Obg, at 11,695 to 12,867
GFF2926
GFF2927 - Permease of the drug/metabolite transporter (DMT) superfamily, at 12,883 to 13,242
GFF2927
GFF2928 - Permease of the drug/metabolite transporter (DMT) superfamily, at 13,248 to 13,847
GFF2928
GFF2929 - LSU ribosomal protein L27p, at 13,977 to 14,234
GFF2929
GFF2930 - LSU ribosomal protein L21p, at 14,254 to 14,565
GFF2930
GFF2931 - Octaprenyl diphosphate synthase (EC 2.5.1.90), at 14,682 to 15,794
GFF2931
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.943 kb on - strand, within GFF2927 at 12.963 kb on - strand, within GFF2927 at 13.075 kb on - strand, within GFF2927 at 13.157 kb on - strand, within GFF2927 at 13.306 kb on - strand at 13.327 kb on + strand, within GFF2928 at 13.379 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.901 kb on - strand at 14.671 kb on - strand at 14.680 kb on - strand at 14.705 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 12,943 - GFF2927 0.17 -0.0 12,963 - GFF2927 0.22 +0.4 13,075 - GFF2927 0.53 +0.0 13,157 - GFF2927 0.76 -0.3 13,306 - -0.6 13,327 + GFF2928 0.13 +0.0 13,379 - GFF2928 0.22 -0.4 13,717 - GFF2928 0.78 -2.1 13,717 - GFF2928 0.78 -0.3 13,717 - GFF2928 0.78 +0.4 13,717 - GFF2928 0.78 -0.7 13,901 - -0.2 14,671 - +0.3 14,680 - -0.6 14,705 + -0.3
Or see this region's nucleotide sequence