Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2914

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2913 and GFF2914 are separated by 85 nucleotidesGFF2914 and GFF2978 are separated by 335 nucleotidesGFF2978 and GFF2915 are separated by 13 nucleotidesGFF2915 and GFF2916 are separated by 222 nucleotides GFF2913 - Argininosuccinate synthase (EC 6.3.4.5), at 399 to 1,742 GFF2913 GFF2914 - putative cytoplasmic protein, at 1,828 to 2,892 GFF2914 GFF2978 - tRNA-Leu-GAG, at 3,228 to 3,314 GFF2978 GFF2915 - Preprotein translocase subunit SecG (TC 3.A.5.1.1), at 3,328 to 3,660 GFF2915 GFF2916 - Phosphoglucosamine mutase (EC 5.4.2.10), at 3,883 to 5,220 GFF2916 Position (kb) 1 2 3Strain fitness (log2 ratio) -1 0 1at 0.859 kb on - strand, within GFF2913at 0.885 kb on - strand, within GFF2913at 0.948 kb on - strand, within GFF2913at 1.542 kb on + strand, within GFF2913at 1.542 kb on + strand, within GFF2913at 1.543 kb on - strand, within GFF2913at 1.543 kb on - strand, within GFF2913at 1.544 kb on + strand, within GFF2913at 1.582 kb on + strand, within GFF2913at 1.791 kb on + strandat 1.897 kb on + strandat 1.898 kb on - strandat 1.898 kb on - strandat 1.898 kb on - strandat 1.899 kb on + strandat 1.998 kb on + strand, within GFF2914at 2.029 kb on - strand, within GFF2914at 2.139 kb on - strand, within GFF2914at 2.224 kb on - strand, within GFF2914at 2.256 kb on + strand, within GFF2914at 2.304 kb on + strand, within GFF2914at 2.305 kb on - strand, within GFF2914at 2.309 kb on - strand, within GFF2914at 2.355 kb on + strand, within GFF2914at 2.356 kb on - strand, within GFF2914at 2.528 kb on + strand, within GFF2914at 2.654 kb on - strand, within GFF2914at 2.656 kb on + strand, within GFF2914at 2.906 kb on - strandat 2.960 kb on - strandat 2.960 kb on - strandat 2.960 kb on - strandat 2.978 kb on + strandat 2.994 kb on + strandat 2.994 kb on + strandat 2.996 kb on + strandat 2.996 kb on + strandat 3.008 kb on + strandat 3.009 kb on - strandat 3.084 kb on + strandat 3.084 kb on + strandat 3.084 kb on + strandat 3.085 kb on - strandat 3.085 kb on - strandat 3.085 kb on - strandat 3.085 kb on - strandat 3.085 kb on - strandat 3.093 kb on - strandat 3.093 kb on - strandat 3.093 kb on - strandat 3.096 kb on + strandat 3.096 kb on + strandat 3.096 kb on + strandat 3.096 kb on + strandat 3.096 kb on + strandat 3.096 kb on + strandat 3.097 kb on - strandat 3.097 kb on - strandat 3.100 kb on + strandat 3.101 kb on - strandat 3.106 kb on + strandat 3.116 kb on + strandat 3.439 kb on - strand, within GFF2915at 3.787 kb on + strandat 3.877 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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859 - GFF2913 0.34 +1.0
885 - GFF2913 0.36 -0.3
948 - GFF2913 0.41 -0.8
1,542 + GFF2913 0.85 -0.6
1,542 + GFF2913 0.85 -0.3
1,543 - GFF2913 0.85 -0.7
1,543 - GFF2913 0.85 +0.0
1,544 + GFF2913 0.85 +0.3
1,582 + GFF2913 0.88 -0.7
1,791 + -0.9
1,897 + -0.5
1,898 - +0.0
1,898 - -0.6
1,898 - +0.0
1,899 + -0.6
1,998 + GFF2914 0.16 -1.0
2,029 - GFF2914 0.19 +0.7
2,139 - GFF2914 0.29 -0.2
2,224 - GFF2914 0.37 -0.9
2,256 + GFF2914 0.40 +0.1
2,304 + GFF2914 0.45 -0.2
2,305 - GFF2914 0.45 -1.0
2,309 - GFF2914 0.45 -1.3
2,355 + GFF2914 0.49 -0.5
2,356 - GFF2914 0.50 +1.6
2,528 + GFF2914 0.66 -0.5
2,654 - GFF2914 0.78 -0.7
2,656 + GFF2914 0.78 -1.4
2,906 - -0.8
2,960 - -0.1
2,960 - -1.1
2,960 - -0.4
2,978 + -0.1
2,994 + +1.3
2,994 + -0.6
2,996 + -1.3
2,996 + -0.9
3,008 + +0.1
3,009 - -0.5
3,084 + -1.1
3,084 + -0.5
3,084 + -0.6
3,085 - -0.3
3,085 - -0.5
3,085 - -0.7
3,085 - -0.7
3,085 - -0.5
3,093 - -0.3
3,093 - -0.9
3,093 - -0.8
3,096 + +1.0
3,096 + +0.0
3,096 + -1.0
3,096 + +0.8
3,096 + -0.6
3,096 + -0.6
3,097 - +0.8
3,097 - -0.9
3,100 + -0.3
3,101 - +0.0
3,106 + +0.1
3,116 + +0.2
3,439 - GFF2915 0.33 +0.8
3,787 + +0.2
3,877 - -0.3

Or see this region's nucleotide sequence