Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2742 and GFF2743 overlap by 4 nucleotides GFF2743 and GFF2744 are separated by 11 nucleotides GFF2744 and GFF2745 are separated by 14 nucleotides
GFF2742 - Oligopeptide transport ATP-binding protein OppF (TC 3.A.1.5.1), at 261,241 to 262,245
GFF2742
GFF2743 - Oligopeptide transport ATP-binding protein OppD (TC 3.A.1.5.1), at 262,242 to 263,249
GFF2743
GFF2744 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 263,261 to 264,169
GFF2744
GFF2745 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 264,184 to 265,104
GFF2745
Position (kb) 262
263
264 Strain fitness (log2 ratio) -1
0
1
2 at 261.404 kb on - strand, within GFF2742 at 261.467 kb on + strand, within GFF2742 at 261.468 kb on - strand, within GFF2742 at 261.483 kb on + strand, within GFF2742 at 261.500 kb on + strand, within GFF2742 at 261.501 kb on - strand, within GFF2742 at 261.501 kb on - strand, within GFF2742 at 261.531 kb on - strand, within GFF2742 at 261.531 kb on - strand, within GFF2742 at 261.531 kb on - strand, within GFF2742 at 261.586 kb on - strand, within GFF2742 at 261.688 kb on - strand, within GFF2742 at 261.854 kb on + strand, within GFF2742 at 261.854 kb on + strand, within GFF2742 at 261.855 kb on - strand, within GFF2742 at 262.009 kb on - strand, within GFF2742 at 262.112 kb on - strand, within GFF2742 at 262.534 kb on - strand, within GFF2743 at 262.634 kb on + strand, within GFF2743 at 262.634 kb on + strand, within GFF2743 at 262.895 kb on + strand, within GFF2743 at 262.898 kb on - strand, within GFF2743 at 262.905 kb on + strand, within GFF2743 at 263.096 kb on + strand, within GFF2743 at 263.101 kb on - strand, within GFF2743 at 263.305 kb on + strand at 263.496 kb on + strand, within GFF2744 at 263.806 kb on - strand, within GFF2744 at 263.856 kb on - strand, within GFF2744 at 264.024 kb on - strand, within GFF2744 at 264.041 kb on - strand, within GFF2744 at 264.161 kb on + strand at 264.162 kb on - strand at 264.162 kb on - strand at 264.216 kb on - strand at 264.242 kb on - strand at 264.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 261,404 - GFF2742 0.16 -0.0 261,467 + GFF2742 0.22 +1.8 261,468 - GFF2742 0.23 +0.3 261,483 + GFF2742 0.24 -0.9 261,500 + GFF2742 0.26 +0.1 261,501 - GFF2742 0.26 +0.7 261,501 - GFF2742 0.26 -0.3 261,531 - GFF2742 0.29 -0.2 261,531 - GFF2742 0.29 -0.5 261,531 - GFF2742 0.29 -0.1 261,586 - GFF2742 0.34 +1.0 261,688 - GFF2742 0.44 -0.4 261,854 + GFF2742 0.61 -0.3 261,854 + GFF2742 0.61 +1.7 261,855 - GFF2742 0.61 -0.1 262,009 - GFF2742 0.76 -0.1 262,112 - GFF2742 0.87 -0.5 262,534 - GFF2743 0.29 -0.4 262,634 + GFF2743 0.39 +1.4 262,634 + GFF2743 0.39 +0.3 262,895 + GFF2743 0.65 +1.0 262,898 - GFF2743 0.65 +0.4 262,905 + GFF2743 0.66 -0.1 263,096 + GFF2743 0.85 +1.1 263,101 - GFF2743 0.85 +0.6 263,305 + +0.3 263,496 + GFF2744 0.26 -1.0 263,806 - GFF2744 0.60 -0.0 263,856 - GFF2744 0.65 +0.2 264,024 - GFF2744 0.84 +0.2 264,041 - GFF2744 0.86 -0.1 264,161 + +0.3 264,162 - +0.3 264,162 - -0.5 264,216 - -0.1 264,242 - -0.2 264,242 - +0.3
Or see this region's nucleotide sequence