Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2721 and GFF2722 are separated by 293 nucleotides GFF2722 and GFF2723 overlap by 1 nucleotides
GFF2721 - COG0613, Predicted metal-dependent phosphoesterases (PHP family), at 243,026 to 243,874
GFF2721
GFF2722 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 244,168 to 245,730
GFF2722
GFF2723 - Anthranilate synthase, amidotransferase component (EC 4.1.3.27) / Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 245,730 to 247,325
GFF2723
Position (kb) 244
245
246 Strain fitness (log2 ratio) -1
0
1 at 243.361 kb on + strand, within GFF2721 at 243.631 kb on + strand, within GFF2721 at 243.809 kb on + strand at 243.810 kb on - strand at 243.914 kb on - strand at 243.914 kb on - strand at 243.914 kb on - strand at 243.928 kb on + strand at 243.929 kb on - strand at 243.929 kb on - strand at 243.929 kb on - strand at 243.936 kb on - strand at 243.951 kb on + strand at 243.951 kb on + strand at 243.953 kb on + strand at 243.962 kb on - strand at 244.025 kb on + strand at 244.087 kb on - strand at 244.335 kb on + strand, within GFF2722 at 244.475 kb on + strand, within GFF2722 at 244.596 kb on + strand, within GFF2722 at 244.596 kb on + strand, within GFF2722 at 244.685 kb on - strand, within GFF2722 at 244.812 kb on + strand, within GFF2722 at 244.823 kb on + strand, within GFF2722 at 244.914 kb on - strand, within GFF2722 at 244.950 kb on - strand, within GFF2722 at 245.009 kb on - strand, within GFF2722 at 245.012 kb on - strand, within GFF2722 at 245.012 kb on - strand, within GFF2722 at 245.012 kb on - strand, within GFF2722 at 245.042 kb on - strand, within GFF2722 at 245.042 kb on - strand, within GFF2722 at 245.043 kb on + strand, within GFF2722 at 245.044 kb on - strand, within GFF2722 at 245.182 kb on - strand, within GFF2722 at 245.236 kb on - strand, within GFF2722 at 245.314 kb on + strand, within GFF2722 at 245.314 kb on + strand, within GFF2722 at 245.315 kb on - strand, within GFF2722 at 245.531 kb on - strand, within GFF2722 at 245.562 kb on + strand, within GFF2722 at 245.563 kb on - strand, within GFF2722 at 245.563 kb on - strand, within GFF2722 at 245.807 kb on + strand at 245.831 kb on + strand at 245.831 kb on + strand at 245.885 kb on - strand at 245.928 kb on - strand, within GFF2723 at 246.010 kb on - strand, within GFF2723 at 246.199 kb on + strand, within GFF2723 at 246.541 kb on - strand, within GFF2723 at 246.581 kb on + strand, within GFF2723 at 246.582 kb on - strand, within GFF2723 at 246.594 kb on - strand, within GFF2723 at 246.710 kb on + strand, within GFF2723
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 243,361 + GFF2721 0.39 +0.8 243,631 + GFF2721 0.71 +0.7 243,809 + +0.7 243,810 - +0.4 243,914 - -0.2 243,914 - -0.7 243,914 - +0.2 243,928 + +0.3 243,929 - +0.1 243,929 - -0.1 243,929 - -0.4 243,936 - -0.2 243,951 + +0.5 243,951 + +1.6 243,953 + +0.4 243,962 - +0.1 244,025 + +0.7 244,087 - -1.3 244,335 + GFF2722 0.11 -0.1 244,475 + GFF2722 0.20 -0.1 244,596 + GFF2722 0.27 -0.5 244,596 + GFF2722 0.27 +0.4 244,685 - GFF2722 0.33 -0.7 244,812 + GFF2722 0.41 +0.5 244,823 + GFF2722 0.42 +0.3 244,914 - GFF2722 0.48 -0.8 244,950 - GFF2722 0.50 +0.1 245,009 - GFF2722 0.54 +0.4 245,012 - GFF2722 0.54 -0.2 245,012 - GFF2722 0.54 +0.6 245,012 - GFF2722 0.54 +1.1 245,042 - GFF2722 0.56 +1.3 245,042 - GFF2722 0.56 +0.4 245,043 + GFF2722 0.56 +1.1 245,044 - GFF2722 0.56 +1.1 245,182 - GFF2722 0.65 +1.1 245,236 - GFF2722 0.68 +1.6 245,314 + GFF2722 0.73 +1.0 245,314 + GFF2722 0.73 -0.3 245,315 - GFF2722 0.73 +0.7 245,531 - GFF2722 0.87 +0.7 245,562 + GFF2722 0.89 -0.0 245,563 - GFF2722 0.89 +0.3 245,563 - GFF2722 0.89 +0.3 245,807 + +0.8 245,831 + +0.1 245,831 + +0.7 245,885 - +0.1 245,928 - GFF2723 0.12 +1.0 246,010 - GFF2723 0.18 +0.7 246,199 + GFF2723 0.29 +1.3 246,541 - GFF2723 0.51 +1.0 246,581 + GFF2723 0.53 +0.8 246,582 - GFF2723 0.53 +0.3 246,594 - GFF2723 0.54 +1.1 246,710 + GFF2723 0.61 +0.3
Or see this region's nucleotide sequence