Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2550 and GFF2551 are separated by 128 nucleotides GFF2551 and GFF2552 are separated by 142 nucleotides GFF2552 and GFF2553 are separated by 349 nucleotides
GFF2550 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 66,334 to 68,118
GFF2550
GFF2551 - Putative outer membrane or secreted lipoprotein, at 68,247 to 69,032
GFF2551
GFF2552 - Putative secreted protein, at 69,175 to 69,504
GFF2552
GFF2553 - Osmotically inducible protein C, at 69,854 to 70,285
GFF2553
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-2
-1
0
1 at 67.276 kb on - strand, within GFF2550 at 67.311 kb on + strand, within GFF2550 at 67.361 kb on + strand, within GFF2550 at 67.422 kb on - strand, within GFF2550 at 67.827 kb on - strand, within GFF2550 at 67.847 kb on + strand, within GFF2550 at 67.928 kb on + strand, within GFF2550 at 68.079 kb on - strand at 68.182 kb on - strand at 68.248 kb on + strand at 68.645 kb on - strand, within GFF2551 at 68.708 kb on + strand, within GFF2551 at 68.713 kb on + strand, within GFF2551 at 68.713 kb on + strand, within GFF2551 at 68.756 kb on - strand, within GFF2551 at 68.797 kb on + strand, within GFF2551 at 68.798 kb on - strand, within GFF2551 at 68.862 kb on - strand, within GFF2551 at 68.862 kb on - strand, within GFF2551 at 68.923 kb on - strand, within GFF2551 at 68.951 kb on + strand, within GFF2551 at 69.420 kb on + strand, within GFF2552 at 69.421 kb on - strand, within GFF2552 at 69.423 kb on + strand, within GFF2552 at 69.423 kb on + strand, within GFF2552 at 69.693 kb on - strand at 69.753 kb on + strand at 69.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 67,276 - GFF2550 0.53 -0.4 67,311 + GFF2550 0.55 +0.6 67,361 + GFF2550 0.58 +0.1 67,422 - GFF2550 0.61 -0.0 67,827 - GFF2550 0.84 -0.6 67,847 + GFF2550 0.85 -1.5 67,928 + GFF2550 0.89 +0.9 68,079 - +0.1 68,182 - +0.8 68,248 + -0.6 68,645 - GFF2551 0.51 -0.6 68,708 + GFF2551 0.59 -0.7 68,713 + GFF2551 0.59 -0.5 68,713 + GFF2551 0.59 -0.2 68,756 - GFF2551 0.65 -0.5 68,797 + GFF2551 0.70 -1.5 68,798 - GFF2551 0.70 -2.2 68,862 - GFF2551 0.78 -0.1 68,862 - GFF2551 0.78 -0.3 68,923 - GFF2551 0.86 -0.8 68,951 + GFF2551 0.90 -0.8 69,420 + GFF2552 0.74 -0.0 69,421 - GFF2552 0.75 +0.3 69,423 + GFF2552 0.75 +0.1 69,423 + GFF2552 0.75 +0.2 69,693 - +0.1 69,753 + -0.5 69,809 - -0.3
Or see this region's nucleotide sequence