Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2324 and GFF2325 are separated by 115 nucleotides GFF2325 and GFF2326 are separated by 2 nucleotides
GFF2324 - Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3), at 109,800 to 112,232
GFF2324
GFF2325 - Small-conductance mechanosensitive channel, at 112,348 to 113,205
GFF2325
GFF2326 - FIG01046298: hypothetical protein, at 113,208 to 114,206
GFF2326
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-1
0
1
2 at 111.542 kb on + strand, within GFF2324 at 111.542 kb on + strand, within GFF2324 at 111.546 kb on + strand, within GFF2324 at 111.546 kb on + strand, within GFF2324 at 111.594 kb on + strand, within GFF2324 at 111.595 kb on - strand, within GFF2324 at 111.596 kb on + strand, within GFF2324 at 111.728 kb on + strand, within GFF2324 at 111.883 kb on + strand, within GFF2324 at 111.884 kb on - strand, within GFF2324 at 111.920 kb on + strand, within GFF2324 at 111.920 kb on + strand, within GFF2324 at 112.103 kb on + strand at 112.234 kb on - strand at 112.310 kb on - strand at 112.310 kb on - strand at 112.315 kb on + strand at 112.316 kb on - strand at 112.317 kb on + strand at 112.318 kb on - strand at 112.405 kb on - strand at 112.457 kb on + strand, within GFF2325 at 112.457 kb on + strand, within GFF2325 at 112.457 kb on + strand, within GFF2325 at 112.458 kb on - strand, within GFF2325 at 112.557 kb on + strand, within GFF2325 at 112.582 kb on - strand, within GFF2325 at 112.591 kb on + strand, within GFF2325 at 112.591 kb on + strand, within GFF2325 at 112.798 kb on + strand, within GFF2325 at 112.933 kb on + strand, within GFF2325 at 112.934 kb on - strand, within GFF2325 at 113.075 kb on - strand, within GFF2325 at 113.246 kb on + strand at 113.247 kb on - strand at 113.328 kb on + strand, within GFF2326 at 113.395 kb on - strand, within GFF2326 at 113.395 kb on - strand, within GFF2326 at 113.984 kb on + strand, within GFF2326 at 114.044 kb on + strand, within GFF2326
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 111,542 + GFF2324 0.72 +0.1 111,542 + GFF2324 0.72 -0.1 111,546 + GFF2324 0.72 -0.2 111,546 + GFF2324 0.72 -0.1 111,594 + GFF2324 0.74 +0.3 111,595 - GFF2324 0.74 +0.4 111,596 + GFF2324 0.74 -0.0 111,728 + GFF2324 0.79 +0.8 111,883 + GFF2324 0.86 -0.1 111,884 - GFF2324 0.86 +2.3 111,920 + GFF2324 0.87 +1.1 111,920 + GFF2324 0.87 +1.5 112,103 + +0.5 112,234 - +0.0 112,310 - -0.8 112,310 - -0.6 112,315 + -0.3 112,316 - +0.4 112,317 + +0.2 112,318 - +0.6 112,405 - +1.1 112,457 + GFF2325 0.13 +0.5 112,457 + GFF2325 0.13 +0.2 112,457 + GFF2325 0.13 +0.5 112,458 - GFF2325 0.13 +1.0 112,557 + GFF2325 0.24 +2.7 112,582 - GFF2325 0.27 -1.3 112,591 + GFF2325 0.28 +1.1 112,591 + GFF2325 0.28 +0.1 112,798 + GFF2325 0.52 -0.0 112,933 + GFF2325 0.68 +0.2 112,934 - GFF2325 0.68 +0.7 113,075 - GFF2325 0.85 +0.1 113,246 + -0.0 113,247 - +0.4 113,328 + GFF2326 0.12 -0.0 113,395 - GFF2326 0.19 -0.0 113,395 - GFF2326 0.19 +0.1 113,984 + GFF2326 0.78 -0.4 114,044 + GFF2326 0.84 -0.1
Or see this region's nucleotide sequence