Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2271 and GFF2272 are separated by 36 nucleotides GFF2272 and GFF2273 are separated by 157 nucleotides GFF2273 and GFF2274 overlap by 8 nucleotides GFF2274 and GFF2275 are separated by 18 nucleotides
GFF2271 - hypothetical protein, at 61,335 to 61,451
GFF2271
GFF2272 - Oligogalacturonide-Rhamnogalacturonide-specific porin, at 61,488 to 62,159
GFF2272
GFF2273 - Predicted rhamnogalacturonide-specific TRAP-type transporter, large transmembrane component RhiC, at 62,317 to 63,624
GFF2273
GFF2274 - Predicted rhamnogalacturonide-specific TRAP-type transporter, small transmembrane component RhiB, at 63,617 to 64,132
GFF2274
GFF2275 - Predicted rhamnogalacturonide-specific TRAP-type transporter, substrate-binding component RhiA, at 64,151 to 65,134
GFF2275
Position (kb) 62
63
64 Strain fitness (log2 ratio) -1
0
1
2 at 61.602 kb on + strand, within GFF2272 at 61.719 kb on - strand, within GFF2272 at 61.736 kb on + strand, within GFF2272 at 61.801 kb on - strand, within GFF2272 at 61.801 kb on - strand, within GFF2272 at 61.873 kb on - strand, within GFF2272 at 61.920 kb on - strand, within GFF2272 at 62.144 kb on - strand at 62.319 kb on - strand at 62.366 kb on + strand at 62.466 kb on - strand, within GFF2273 at 62.468 kb on - strand, within GFF2273 at 62.513 kb on + strand, within GFF2273 at 62.513 kb on + strand, within GFF2273 at 62.513 kb on + strand, within GFF2273 at 62.691 kb on + strand, within GFF2273 at 62.713 kb on - strand, within GFF2273 at 62.855 kb on - strand, within GFF2273 at 62.861 kb on + strand, within GFF2273 at 62.979 kb on + strand, within GFF2273 at 63.046 kb on - strand, within GFF2273 at 63.136 kb on - strand, within GFF2273 at 63.301 kb on - strand, within GFF2273 at 63.319 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.592 kb on + strand at 63.592 kb on + strand at 63.598 kb on - strand at 63.613 kb on - strand at 63.705 kb on + strand, within GFF2274 at 63.705 kb on + strand, within GFF2274 at 63.925 kb on + strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 64.104 kb on - strand at 64.148 kb on - strand at 64.286 kb on + strand, within GFF2275 at 64.412 kb on - strand, within GFF2275 at 64.458 kb on + strand, within GFF2275 at 64.459 kb on - strand, within GFF2275 at 64.539 kb on - strand, within GFF2275 at 64.539 kb on - strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.549 kb on - strand, within GFF2275
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 61,602 + GFF2272 0.17 -0.2 61,719 - GFF2272 0.34 +0.2 61,736 + GFF2272 0.37 -0.1 61,801 - GFF2272 0.47 +0.2 61,801 - GFF2272 0.47 +0.2 61,873 - GFF2272 0.57 -0.0 61,920 - GFF2272 0.64 -1.5 62,144 - +0.3 62,319 - -0.8 62,366 + -0.4 62,466 - GFF2273 0.11 +0.1 62,468 - GFF2273 0.12 +0.0 62,513 + GFF2273 0.15 +0.1 62,513 + GFF2273 0.15 +1.3 62,513 + GFF2273 0.15 +0.0 62,691 + GFF2273 0.29 +0.7 62,713 - GFF2273 0.30 +0.1 62,855 - GFF2273 0.41 +0.9 62,861 + GFF2273 0.42 -1.0 62,979 + GFF2273 0.51 +0.4 63,046 - GFF2273 0.56 -0.1 63,136 - GFF2273 0.63 -0.1 63,301 - GFF2273 0.75 +1.8 63,319 - GFF2273 0.77 +0.3 63,321 - GFF2273 0.77 +0.4 63,321 - GFF2273 0.77 +0.5 63,321 - GFF2273 0.77 +0.2 63,592 + +0.8 63,592 + +0.2 63,598 - -1.1 63,613 - +0.2 63,705 + GFF2274 0.17 +0.4 63,705 + GFF2274 0.17 +0.2 63,925 + GFF2274 0.60 -0.3 63,926 - GFF2274 0.60 +0.6 63,926 - GFF2274 0.60 +0.2 63,926 - GFF2274 0.60 +0.0 64,104 - +0.7 64,148 - +0.1 64,286 + GFF2275 0.14 +0.4 64,412 - GFF2275 0.27 -0.5 64,458 + GFF2275 0.31 +0.3 64,459 - GFF2275 0.31 -0.1 64,539 - GFF2275 0.39 +0.5 64,539 - GFF2275 0.39 +0.7 64,546 + GFF2275 0.40 +0.7 64,546 + GFF2275 0.40 +0.7 64,547 - GFF2275 0.40 +0.8 64,547 - GFF2275 0.40 +0.5 64,548 + GFF2275 0.40 +0.2 64,548 + GFF2275 0.40 +0.3 64,549 - GFF2275 0.40 -0.1
Or see this region's nucleotide sequence