Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2354 and GFF2209 are separated by 262 nucleotides GFF2209 and GFF2210 overlap by 19 nucleotides GFF2210 and GFF2211 are separated by 69 nucleotides GFF2211 and GFF2212 are separated by 76 nucleotides
GFF2354 - 5S RNA, at 226 to 345
GFF2354
GFF2209 - Molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 608 to 1,138
GFF2209
GFF2210 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 1,120 to 1,704
GFF2210
GFF2211 - Protein yihD, at 1,774 to 2,043
GFF2211
GFF2212 - YihE protein, a ser/thr kinase implicated in LPS synthesis and Cpx signalling, at 2,120 to 3,106
GFF2212
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 0.031 kb on + strand at 0.104 kb on + strand at 0.104 kb on + strand at 0.145 kb on + strand at 0.153 kb on + strand at 0.153 kb on + strand at 0.153 kb on + strand at 0.528 kb on - strand at 0.662 kb on + strand, within GFF2209 at 0.670 kb on + strand, within GFF2209 at 0.671 kb on - strand, within GFF2209 at 0.818 kb on + strand, within GFF2209 at 0.830 kb on - strand, within GFF2209 at 0.956 kb on - strand, within GFF2209 at 0.967 kb on - strand, within GFF2209 at 0.971 kb on - strand, within GFF2209 at 0.971 kb on - strand, within GFF2209 at 1.009 kb on + strand, within GFF2209 at 1.016 kb on - strand, within GFF2209 at 1.112 kb on + strand at 1.182 kb on + strand, within GFF2210 at 1.183 kb on - strand, within GFF2210 at 1.183 kb on - strand, within GFF2210 at 1.259 kb on + strand, within GFF2210 at 1.259 kb on + strand, within GFF2210 at 1.259 kb on + strand, within GFF2210 at 1.259 kb on + strand, within GFF2210 at 1.260 kb on - strand, within GFF2210 at 1.260 kb on - strand, within GFF2210 at 1.290 kb on + strand, within GFF2210 at 1.291 kb on - strand, within GFF2210 at 1.435 kb on + strand, within GFF2210 at 1.560 kb on + strand, within GFF2210 at 1.684 kb on + strand at 1.685 kb on - strand at 1.687 kb on + strand at 1.688 kb on - strand at 1.688 kb on - strand at 1.720 kb on + strand at 1.720 kb on + strand at 1.842 kb on + strand, within GFF2211 at 1.927 kb on + strand, within GFF2211 at 1.928 kb on - strand, within GFF2211 at 2.034 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 31 + -1.8 104 + -0.5 104 + -0.9 145 + +0.1 153 + +0.4 153 + -0.8 153 + -0.5 528 - -0.1 662 + GFF2209 0.10 +0.2 670 + GFF2209 0.12 +2.8 671 - GFF2209 0.12 -0.7 818 + GFF2209 0.40 -0.2 830 - GFF2209 0.42 -0.5 956 - GFF2209 0.66 -0.9 967 - GFF2209 0.68 -0.8 971 - GFF2209 0.68 -2.1 971 - GFF2209 0.68 -1.2 1,009 + GFF2209 0.76 +0.4 1,016 - GFF2209 0.77 +0.2 1,112 + -0.3 1,182 + GFF2210 0.11 -0.5 1,183 - GFF2210 0.11 -0.8 1,183 - GFF2210 0.11 -0.5 1,259 + GFF2210 0.24 -0.1 1,259 + GFF2210 0.24 -0.3 1,259 + GFF2210 0.24 +0.3 1,259 + GFF2210 0.24 -1.3 1,260 - GFF2210 0.24 -0.1 1,260 - GFF2210 0.24 -0.3 1,290 + GFF2210 0.29 +0.4 1,291 - GFF2210 0.29 -0.8 1,435 + GFF2210 0.54 +1.2 1,560 + GFF2210 0.75 +0.1 1,684 + -1.8 1,685 - +0.8 1,687 + -0.8 1,688 - -0.1 1,688 - -0.5 1,720 + -0.3 1,720 + -0.9 1,842 + GFF2211 0.25 -0.1 1,927 + GFF2211 0.57 -0.3 1,928 - GFF2211 0.57 +0.0 2,034 + -0.3
Or see this region's nucleotide sequence