Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1809 and GFF1810 are separated by 188 nucleotides GFF1810 and GFF1811 are separated by 365 nucleotides GFF1811 and GFF1812 are separated by 74 nucleotides
GFF1809 - Putative GTP-binding protein YdgA, at 33,076 to 34,584
GFF1809
GFF1810 - FIG01046382: hypothetical protein, at 34,773 to 34,922
GFF1810
GFF1811 - Adenosine deaminase (EC 3.5.4.4), at 35,288 to 36,289
GFF1811
GFF1812 - Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-), at 36,364 to 37,404
GFF1812
Position (kb)
35
36
37 Strain fitness (log2 ratio)
-1
0
1 at 34.447 kb on - strand at 34.447 kb on - strand at 34.469 kb on - strand at 34.644 kb on - strand at 34.825 kb on + strand, within GFF1810 at 34.852 kb on + strand, within GFF1810 at 34.852 kb on + strand, within GFF1810 at 34.920 kb on + strand at 34.980 kb on + strand at 35.031 kb on - strand at 35.102 kb on - strand at 35.247 kb on - strand at 35.251 kb on + strand at 35.296 kb on + strand at 35.299 kb on + strand at 35.299 kb on + strand at 35.713 kb on + strand, within GFF1811 at 35.737 kb on - strand, within GFF1811 at 35.737 kb on - strand, within GFF1811 at 35.867 kb on - strand, within GFF1811 at 35.955 kb on + strand, within GFF1811 at 36.142 kb on - strand, within GFF1811 at 36.298 kb on + strand at 36.338 kb on + strand at 36.497 kb on + strand, within GFF1812 at 36.497 kb on + strand, within GFF1812 at 36.500 kb on + strand, within GFF1812 at 36.510 kb on - strand, within GFF1812 at 36.693 kb on - strand, within GFF1812 at 37.229 kb on - strand, within GFF1812
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 34,447 - -0.4 34,447 - -0.9 34,469 - +0.1 34,644 - -0.6 34,825 + GFF1810 0.35 -0.2 34,852 + GFF1810 0.53 -0.6 34,852 + GFF1810 0.53 +0.3 34,920 + -0.3 34,980 + -0.1 35,031 - +0.6 35,102 - -1.0 35,247 - -0.3 35,251 + -0.4 35,296 + -0.6 35,299 + +0.5 35,299 + +1.2 35,713 + GFF1811 0.42 +0.5 35,737 - GFF1811 0.45 +0.3 35,737 - GFF1811 0.45 -0.3 35,867 - GFF1811 0.58 +1.0 35,955 + GFF1811 0.67 +0.3 36,142 - GFF1811 0.85 +0.2 36,298 + -0.2 36,338 + -0.8 36,497 + GFF1812 0.13 +0.0 36,497 + GFF1812 0.13 +0.0 36,500 + GFF1812 0.13 -0.2 36,510 - GFF1812 0.14 -0.3 36,693 - GFF1812 0.32 +0.5 37,229 - GFF1812 0.83 +0.9
Or see this region's nucleotide sequence