Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1760 and GFF1761 are separated by 305 nucleotides GFF1761 and GFF1762 are separated by 260 nucleotides GFF1762 and GFF1763 overlap by 4 nucleotides
GFF1760 - Putative permease, at 63,221 to 64,483
GFF1760
GFF1761 - Promiscuous sugar phosphatase YidA, haloacid dehalogenase-like phosphatase family, at 64,789 to 65,634
GFF1761
GFF1762 - D-Galactonate repressor DgoR, at 65,895 to 66,584
GFF1762
GFF1763 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 66,581 to 67,459
GFF1763
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 64.266 kb on - strand, within GFF1760 at 64.648 kb on + strand at 64.797 kb on + strand at 64.946 kb on - strand, within GFF1761 at 64.981 kb on + strand, within GFF1761 at 64.994 kb on - strand, within GFF1761 at 64.996 kb on - strand, within GFF1761 at 65.056 kb on + strand, within GFF1761 at 65.056 kb on + strand, within GFF1761 at 65.057 kb on - strand, within GFF1761 at 65.065 kb on + strand, within GFF1761 at 65.072 kb on - strand, within GFF1761 at 65.118 kb on - strand, within GFF1761 at 65.135 kb on - strand, within GFF1761 at 65.158 kb on + strand, within GFF1761 at 65.162 kb on - strand, within GFF1761 at 65.162 kb on - strand, within GFF1761 at 65.162 kb on - strand, within GFF1761 at 65.180 kb on - strand, within GFF1761 at 65.250 kb on + strand, within GFF1761 at 65.419 kb on - strand, within GFF1761 at 65.419 kb on - strand, within GFF1761 at 65.544 kb on + strand, within GFF1761 at 65.715 kb on - strand at 65.819 kb on + strand at 65.819 kb on + strand at 65.820 kb on - strand at 65.862 kb on + strand at 65.862 kb on + strand at 65.862 kb on + strand at 65.863 kb on - strand at 65.863 kb on - strand at 65.906 kb on + strand at 65.954 kb on + strand at 65.954 kb on + strand at 65.954 kb on + strand at 66.121 kb on - strand, within GFF1762 at 66.221 kb on - strand, within GFF1762 at 66.300 kb on - strand, within GFF1762 at 66.388 kb on + strand, within GFF1762 at 66.388 kb on + strand, within GFF1762 at 66.410 kb on - strand, within GFF1762 at 66.421 kb on + strand, within GFF1762 at 66.479 kb on + strand, within GFF1762 at 66.479 kb on + strand, within GFF1762 at 66.479 kb on + strand, within GFF1762 at 66.480 kb on - strand, within GFF1762 at 66.593 kb on + strand at 66.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 64,266 - GFF1760 0.83 -0.1 64,648 + -0.6 64,797 + +0.5 64,946 - GFF1761 0.19 -0.4 64,981 + GFF1761 0.23 -0.8 64,994 - GFF1761 0.24 -0.5 64,996 - GFF1761 0.24 -0.5 65,056 + GFF1761 0.32 -0.0 65,056 + GFF1761 0.32 -0.2 65,057 - GFF1761 0.32 +0.0 65,065 + GFF1761 0.33 -0.0 65,072 - GFF1761 0.33 -0.5 65,118 - GFF1761 0.39 -0.5 65,135 - GFF1761 0.41 -0.1 65,158 + GFF1761 0.44 -0.2 65,162 - GFF1761 0.44 +0.1 65,162 - GFF1761 0.44 -0.6 65,162 - GFF1761 0.44 -0.5 65,180 - GFF1761 0.46 +0.1 65,250 + GFF1761 0.54 -0.3 65,419 - GFF1761 0.74 -0.4 65,419 - GFF1761 0.74 -0.1 65,544 + GFF1761 0.89 -0.8 65,715 - -0.1 65,819 + -1.0 65,819 + -0.2 65,820 - -0.2 65,862 + -0.1 65,862 + -0.2 65,862 + +0.7 65,863 - -0.1 65,863 - -0.5 65,906 + +2.2 65,954 + -2.1 65,954 + +0.5 65,954 + -0.3 66,121 - GFF1762 0.33 -0.8 66,221 - GFF1762 0.47 -0.9 66,300 - GFF1762 0.59 +0.4 66,388 + GFF1762 0.71 -0.4 66,388 + GFF1762 0.71 -2.3 66,410 - GFF1762 0.75 -0.0 66,421 + GFF1762 0.76 -0.4 66,479 + GFF1762 0.85 -0.2 66,479 + GFF1762 0.85 -0.5 66,479 + GFF1762 0.85 -0.3 66,480 - GFF1762 0.85 +0.8 66,593 + -0.0 66,594 - -0.2
Or see this region's nucleotide sequence