Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1716 and GFF1717 are separated by 102 nucleotides GFF1717 and GFF1718 are separated by 357 nucleotides GFF1718 and GFF1719 are separated by 267 nucleotides
GFF1716 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 16,052 to 17,941
GFF1716
GFF1717 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 18,044 to 18,667
GFF1717
GFF1718 - hypothetical protein, at 19,025 to 19,168
GFF1718
GFF1719 - ATP synthase protein I, at 19,436 to 19,663
GFF1719
Position (kb) 18
19 Strain fitness (log2 ratio) -1
0
1
2 at 17.338 kb on + strand, within GFF1716 at 17.610 kb on - strand, within GFF1716 at 17.702 kb on + strand, within GFF1716 at 17.739 kb on + strand, within GFF1716 at 17.739 kb on + strand, within GFF1716 at 17.753 kb on - strand at 17.753 kb on - strand at 17.753 kb on - strand at 17.830 kb on + strand at 17.830 kb on + strand at 17.831 kb on - strand at 17.920 kb on + strand at 17.920 kb on + strand at 17.921 kb on - strand at 17.921 kb on - strand at 17.921 kb on - strand at 17.948 kb on + strand at 17.949 kb on - strand at 18.122 kb on + strand, within GFF1717 at 18.130 kb on + strand, within GFF1717 at 18.130 kb on + strand, within GFF1717 at 18.155 kb on + strand, within GFF1717 at 18.155 kb on + strand, within GFF1717 at 18.155 kb on + strand, within GFF1717 at 18.156 kb on - strand, within GFF1717 at 18.216 kb on + strand, within GFF1717 at 18.482 kb on - strand, within GFF1717 at 18.584 kb on - strand, within GFF1717 at 19.014 kb on - strand at 19.063 kb on + strand, within GFF1718 at 19.140 kb on + strand, within GFF1718 at 19.218 kb on + strand at 19.318 kb on + strand at 19.318 kb on + strand at 19.350 kb on + strand at 19.432 kb on + strand at 19.435 kb on + strand at 19.435 kb on + strand at 19.435 kb on + strand at 19.474 kb on + strand, within GFF1719 at 19.474 kb on + strand, within GFF1719 at 19.474 kb on + strand, within GFF1719 at 19.548 kb on + strand, within GFF1719 at 19.572 kb on + strand, within GFF1719 at 19.644 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 17,338 + GFF1716 0.68 +1.0 17,610 - GFF1716 0.82 +0.7 17,702 + GFF1716 0.87 +0.3 17,739 + GFF1716 0.89 +0.4 17,739 + GFF1716 0.89 +0.6 17,753 - -0.3 17,753 - +0.5 17,753 - +0.9 17,830 + -0.0 17,830 + +0.6 17,831 - -0.8 17,920 + +0.1 17,920 + -0.1 17,921 - +1.2 17,921 - -0.1 17,921 - -0.1 17,948 + +0.0 17,949 - +0.8 18,122 + GFF1717 0.12 +0.7 18,130 + GFF1717 0.14 +1.1 18,130 + GFF1717 0.14 +0.9 18,155 + GFF1717 0.18 +0.2 18,155 + GFF1717 0.18 +1.8 18,155 + GFF1717 0.18 -0.6 18,156 - GFF1717 0.18 +1.5 18,216 + GFF1717 0.28 +2.0 18,482 - GFF1717 0.70 +0.2 18,584 - GFF1717 0.87 +0.6 19,014 - +0.5 19,063 + GFF1718 0.26 +0.0 19,140 + GFF1718 0.80 +0.1 19,218 + +1.1 19,318 + -0.5 19,318 + -0.2 19,350 + +0.7 19,432 + -0.8 19,435 + -0.2 19,435 + +1.2 19,435 + -0.5 19,474 + GFF1719 0.17 -0.4 19,474 + GFF1719 0.17 +0.1 19,474 + GFF1719 0.17 -1.2 19,548 + GFF1719 0.49 +0.0 19,572 + GFF1719 0.60 +0.2 19,644 + -0.6
Or see this region's nucleotide sequence