Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1532 and GFF1533 are separated by 10 nucleotides GFF1533 and GFF1534 are separated by 239 nucleotides
GFF1532 - Transcriptional repressor of aga operon, at 36,287 to 37,120
GFF1532
GFF1533 - Fructokinase (EC 2.7.1.4), at 37,131 to 38,078
GFF1533
GFF1534 - D-galactarate dehydratase (EC 4.2.1.42), at 38,318 to 39,889
GFF1534
Position (kb) 37
38
39 Strain fitness (log2 ratio) -2
-1
0
1
2 at 36.227 kb on + strand at 36.592 kb on + strand, within GFF1532 at 36.593 kb on - strand, within GFF1532 at 36.836 kb on - strand, within GFF1532 at 37.000 kb on + strand, within GFF1532 at 37.064 kb on + strand at 37.064 kb on + strand at 37.065 kb on - strand at 37.065 kb on - strand at 37.139 kb on - strand at 37.139 kb on - strand at 37.139 kb on - strand at 37.299 kb on - strand, within GFF1533 at 37.598 kb on - strand, within GFF1533 at 37.839 kb on + strand, within GFF1533 at 37.840 kb on - strand, within GFF1533 at 37.840 kb on - strand, within GFF1533 at 37.982 kb on + strand, within GFF1533 at 37.983 kb on - strand, within GFF1533 at 38.003 kb on + strand at 38.076 kb on + strand at 38.080 kb on + strand at 38.090 kb on + strand at 38.104 kb on + strand at 38.105 kb on - strand at 38.115 kb on + strand at 38.423 kb on + strand at 38.424 kb on - strand at 38.489 kb on + strand, within GFF1534 at 38.489 kb on + strand, within GFF1534 at 38.490 kb on - strand, within GFF1534 at 38.496 kb on + strand, within GFF1534 at 38.497 kb on - strand, within GFF1534 at 38.591 kb on + strand, within GFF1534 at 38.597 kb on - strand, within GFF1534 at 38.655 kb on + strand, within GFF1534 at 38.656 kb on - strand, within GFF1534 at 38.656 kb on - strand, within GFF1534 at 38.819 kb on - strand, within GFF1534 at 38.826 kb on + strand, within GFF1534 at 38.826 kb on + strand, within GFF1534 at 38.826 kb on + strand, within GFF1534 at 38.856 kb on - strand, within GFF1534
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 36,227 + -0.1 36,592 + GFF1532 0.37 -1.8 36,593 - GFF1532 0.37 -0.4 36,836 - GFF1532 0.66 -0.2 37,000 + GFF1532 0.85 +0.0 37,064 + +0.2 37,064 + -0.2 37,065 - +0.0 37,065 - -0.4 37,139 - -0.5 37,139 - +0.6 37,139 - -0.4 37,299 - GFF1533 0.18 -0.2 37,598 - GFF1533 0.49 -0.0 37,839 + GFF1533 0.75 +0.2 37,840 - GFF1533 0.75 +0.2 37,840 - GFF1533 0.75 +0.4 37,982 + GFF1533 0.90 -0.9 37,983 - GFF1533 0.90 -0.4 38,003 + +0.3 38,076 + +0.8 38,080 + -0.7 38,090 + -0.3 38,104 + -0.5 38,105 - +0.5 38,115 + -0.2 38,423 + -0.0 38,424 - +0.0 38,489 + GFF1534 0.11 -0.8 38,489 + GFF1534 0.11 +0.1 38,490 - GFF1534 0.11 -0.2 38,496 + GFF1534 0.11 +2.0 38,497 - GFF1534 0.11 +0.1 38,591 + GFF1534 0.17 -0.5 38,597 - GFF1534 0.18 +0.2 38,655 + GFF1534 0.21 +0.4 38,656 - GFF1534 0.22 +1.7 38,656 - GFF1534 0.22 -0.4 38,819 - GFF1534 0.32 -0.0 38,826 + GFF1534 0.32 +0.6 38,826 + GFF1534 0.32 +0.0 38,826 + GFF1534 0.32 +0.0 38,856 - GFF1534 0.34 -0.2
Or see this region's nucleotide sequence