Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1517 and GFF1518 are separated by 9 nucleotides GFF1518 and GFF1519 are separated by 21 nucleotides GFF1519 and GFF1520 overlap by 43 nucleotides GFF1520 and GFF1521 are separated by 63 nucleotides
GFF1517 - 21 kDa hemolysin precursor, at 21,639 to 22,214
GFF1517
GFF1518 - Phosphoheptose isomerase (EC 5.3.1.-), at 22,224 to 22,814
GFF1518
GFF1519 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 22,836 to 23,231
GFF1519
GFF1520 - LppC putative lipoprotein, at 23,189 to 25,231
GFF1520
GFF1521 - rRNA small subunit methyltransferase I, at 25,295 to 26,158
GFF1521
Position (kb)
23
24
25
26 Strain fitness (log2 ratio)
-1
0
1
2 at 22.226 kb on - strand at 22.226 kb on - strand at 22.290 kb on - strand, within GFF1518 at 22.356 kb on + strand, within GFF1518 at 22.357 kb on - strand, within GFF1518 at 22.558 kb on - strand, within GFF1518 at 22.817 kb on + strand at 22.893 kb on + strand, within GFF1519 at 22.961 kb on + strand, within GFF1519 at 22.962 kb on - strand, within GFF1519 at 22.962 kb on - strand, within GFF1519 at 22.969 kb on - strand, within GFF1519 at 23.364 kb on + strand at 23.394 kb on - strand, within GFF1520 at 23.458 kb on - strand, within GFF1520 at 23.462 kb on + strand, within GFF1520 at 23.463 kb on - strand, within GFF1520 at 23.611 kb on - strand, within GFF1520 at 23.911 kb on - strand, within GFF1520 at 23.915 kb on - strand, within GFF1520 at 23.915 kb on - strand, within GFF1520 at 23.961 kb on + strand, within GFF1520 at 23.962 kb on - strand, within GFF1520 at 23.962 kb on - strand, within GFF1520 at 23.968 kb on + strand, within GFF1520 at 23.968 kb on + strand, within GFF1520 at 23.969 kb on - strand, within GFF1520 at 23.969 kb on - strand, within GFF1520 at 23.971 kb on + strand, within GFF1520 at 23.980 kb on + strand, within GFF1520 at 23.980 kb on + strand, within GFF1520 at 23.980 kb on + strand, within GFF1520 at 23.985 kb on - strand, within GFF1520 at 24.185 kb on - strand, within GFF1520 at 24.656 kb on + strand, within GFF1520 at 24.858 kb on - strand, within GFF1520 at 24.858 kb on - strand, within GFF1520 at 24.923 kb on + strand, within GFF1520 at 25.032 kb on - strand at 25.118 kb on - strand at 25.118 kb on - strand at 25.119 kb on + strand at 25.120 kb on - strand at 25.232 kb on - strand at 25.232 kb on - strand at 25.615 kb on + strand, within GFF1521 at 25.615 kb on + strand, within GFF1521 at 25.616 kb on - strand, within GFF1521 at 25.822 kb on - strand, within GFF1521 at 25.865 kb on + strand, within GFF1521 at 26.127 kb on - strand at 26.129 kb on - strand at 26.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 22,226 - -0.4 22,226 - -0.1 22,290 - GFF1518 0.11 +0.7 22,356 + GFF1518 0.22 +0.7 22,357 - GFF1518 0.23 +0.6 22,558 - GFF1518 0.57 +0.9 22,817 + +0.9 22,893 + GFF1519 0.14 +0.4 22,961 + GFF1519 0.32 +0.9 22,962 - GFF1519 0.32 -0.1 22,962 - GFF1519 0.32 +1.6 22,969 - GFF1519 0.34 -0.2 23,364 + +1.0 23,394 - GFF1520 0.10 +0.5 23,458 - GFF1520 0.13 +0.1 23,462 + GFF1520 0.13 +1.1 23,463 - GFF1520 0.13 -0.2 23,611 - GFF1520 0.21 -0.0 23,911 - GFF1520 0.35 -0.8 23,915 - GFF1520 0.36 +2.5 23,915 - GFF1520 0.36 +0.7 23,961 + GFF1520 0.38 +1.1 23,962 - GFF1520 0.38 +0.4 23,962 - GFF1520 0.38 +1.1 23,968 + GFF1520 0.38 +0.5 23,968 + GFF1520 0.38 +0.5 23,969 - GFF1520 0.38 -0.1 23,969 - GFF1520 0.38 +0.1 23,971 + GFF1520 0.38 +0.9 23,980 + GFF1520 0.39 -0.3 23,980 + GFF1520 0.39 +0.6 23,980 + GFF1520 0.39 +0.6 23,985 - GFF1520 0.39 +0.5 24,185 - GFF1520 0.49 +1.4 24,656 + GFF1520 0.72 -0.3 24,858 - GFF1520 0.82 +1.8 24,858 - GFF1520 0.82 +0.7 24,923 + GFF1520 0.85 +0.2 25,032 - +0.6 25,118 - +0.6 25,118 - +2.1 25,119 + +0.0 25,120 - -0.3 25,232 - +0.0 25,232 - +0.6 25,615 + GFF1521 0.37 -0.2 25,615 + GFF1521 0.37 +0.2 25,616 - GFF1521 0.37 -0.1 25,822 - GFF1521 0.61 +0.6 25,865 + GFF1521 0.66 -0.7 26,127 - -0.9 26,129 - +0.1 26,202 - +1.1
Or see this region's nucleotide sequence