Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1498 and GFF1499 are separated by 220 nucleotides GFF1499 and GFF1500 overlap by 1 nucleotides GFF1500 and GFF1501 are separated by 150 nucleotides GFF1501 and GFF1502 are separated by 211 nucleotides
GFF1498 - Translation initiation factor 2, at 2,270 to 4,948
GFF1498
GFF1499 - Ribosome-binding factor A, at 5,169 to 5,570
GFF1499
GFF1500 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 5,570 to 6,514
GFF1500
GFF1501 - SSU ribosomal protein S15p (S13e), at 6,665 to 6,934
GFF1501
GFF1502 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 7,146 to 9,311
GFF1502
Position (kb) 5
6
7 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5.006 kb on + strand at 5.006 kb on + strand at 5.006 kb on + strand at 5.014 kb on + strand at 5.014 kb on + strand at 5.014 kb on + strand at 5.014 kb on + strand at 5.014 kb on + strand at 5.076 kb on + strand at 5.492 kb on + strand, within GFF1499 at 5.510 kb on + strand, within GFF1499 at 5.510 kb on + strand, within GFF1499 at 5.510 kb on + strand, within GFF1499 at 5.511 kb on - strand, within GFF1499 at 5.772 kb on - strand, within GFF1500 at 5.795 kb on + strand, within GFF1500 at 5.795 kb on + strand, within GFF1500 at 5.961 kb on - strand, within GFF1500 at 5.984 kb on + strand, within GFF1500 at 6.035 kb on + strand, within GFF1500 at 6.104 kb on + strand, within GFF1500 at 6.104 kb on + strand, within GFF1500 at 6.104 kb on + strand, within GFF1500 at 6.104 kb on + strand, within GFF1500 at 6.105 kb on - strand, within GFF1500 at 6.105 kb on - strand, within GFF1500 at 6.113 kb on - strand, within GFF1500 at 6.129 kb on + strand, within GFF1500 at 6.159 kb on + strand, within GFF1500 at 6.159 kb on + strand, within GFF1500 at 6.160 kb on - strand, within GFF1500 at 6.164 kb on + strand, within GFF1500 at 6.289 kb on - strand, within GFF1500 at 6.332 kb on - strand, within GFF1500 at 6.332 kb on - strand, within GFF1500 at 6.354 kb on + strand, within GFF1500 at 6.357 kb on - strand, within GFF1500 at 6.382 kb on + strand, within GFF1500 at 6.495 kb on + strand at 6.498 kb on + strand at 6.498 kb on + strand at 6.499 kb on - strand at 6.512 kb on + strand at 6.512 kb on + strand at 6.513 kb on - strand at 6.580 kb on + strand at 6.933 kb on - strand at 7.010 kb on - strand at 7.014 kb on + strand at 7.015 kb on - strand at 7.015 kb on - strand at 7.098 kb on + strand at 7.197 kb on - strand at 7.273 kb on + strand at 7.362 kb on + strand at 7.363 kb on - strand, within GFF1502 at 7.373 kb on + strand, within GFF1502
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 5,006 + +0.1 5,006 + -0.3 5,006 + -0.6 5,014 + -1.6 5,014 + +0.1 5,014 + +0.1 5,014 + +0.2 5,014 + +0.3 5,076 + -0.2 5,492 + GFF1499 0.80 +1.2 5,510 + GFF1499 0.85 +1.4 5,510 + GFF1499 0.85 +1.8 5,510 + GFF1499 0.85 +1.2 5,511 - GFF1499 0.85 +0.8 5,772 - GFF1500 0.21 +1.0 5,795 + GFF1500 0.24 +1.5 5,795 + GFF1500 0.24 +1.3 5,961 - GFF1500 0.41 +2.2 5,984 + GFF1500 0.44 +1.8 6,035 + GFF1500 0.49 +1.2 6,104 + GFF1500 0.57 +1.5 6,104 + GFF1500 0.57 +1.7 6,104 + GFF1500 0.57 +1.0 6,104 + GFF1500 0.57 +1.0 6,105 - GFF1500 0.57 +0.8 6,105 - GFF1500 0.57 +1.6 6,113 - GFF1500 0.57 +1.2 6,129 + GFF1500 0.59 +1.2 6,159 + GFF1500 0.62 +3.4 6,159 + GFF1500 0.62 +0.2 6,160 - GFF1500 0.62 +0.6 6,164 + GFF1500 0.63 +1.0 6,289 - GFF1500 0.76 +1.4 6,332 - GFF1500 0.81 +1.4 6,332 - GFF1500 0.81 +0.4 6,354 + GFF1500 0.83 +1.5 6,357 - GFF1500 0.83 +0.6 6,382 + GFF1500 0.86 +1.1 6,495 + +0.9 6,498 + -1.1 6,498 + -0.4 6,499 - -0.9 6,512 + -0.1 6,512 + -2.4 6,513 - -0.3 6,580 + +0.3 6,933 - +2.1 7,010 - +0.8 7,014 + +0.1 7,015 - +0.6 7,015 - -0.6 7,098 + -0.0 7,197 - -0.7 7,273 + +0.6 7,362 + -0.3 7,363 - GFF1502 0.10 +0.6 7,373 + GFF1502 0.10 +0.3
Or see this region's nucleotide sequence