Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1323 and GFF1324 are separated by 117 nucleotides GFF1324 and GFF1325 overlap by 29 nucleotides GFF1325 and GFF1326 are separated by 112 nucleotides
GFF1323 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 31,503 to 32,765
GFF1323
GFF1324 - Transcriptional activator protein LysR, at 32,883 to 33,818
GFF1324
GFF1325 - Aspartate racemase (EC 5.1.1.13), at 33,790 to 34,497
GFF1325
GFF1326 - Arabinose-proton symporter, at 34,610 to 36,028
GFF1326
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-1
0
1 at 32.046 kb on - strand, within GFF1323 at 32.198 kb on + strand, within GFF1323 at 32.198 kb on + strand, within GFF1323 at 32.225 kb on - strand, within GFF1323 at 32.390 kb on + strand, within GFF1323 at 32.417 kb on + strand, within GFF1323 at 32.418 kb on - strand, within GFF1323 at 32.586 kb on - strand, within GFF1323 at 32.586 kb on - strand, within GFF1323 at 32.750 kb on - strand at 32.765 kb on + strand at 32.766 kb on - strand at 32.883 kb on - strand at 33.055 kb on + strand, within GFF1324 at 33.094 kb on + strand, within GFF1324 at 33.220 kb on + strand, within GFF1324 at 33.261 kb on - strand, within GFF1324 at 33.370 kb on + strand, within GFF1324 at 33.371 kb on - strand, within GFF1324 at 33.394 kb on + strand, within GFF1324 at 33.506 kb on + strand, within GFF1324 at 33.511 kb on + strand, within GFF1324 at 33.612 kb on - strand, within GFF1324 at 33.744 kb on - strand at 33.816 kb on + strand at 33.817 kb on - strand at 33.898 kb on + strand, within GFF1325 at 33.899 kb on - strand, within GFF1325 at 33.968 kb on + strand, within GFF1325 at 34.117 kb on - strand, within GFF1325 at 34.286 kb on - strand, within GFF1325 at 34.348 kb on + strand, within GFF1325 at 34.349 kb on - strand, within GFF1325 at 34.369 kb on + strand, within GFF1325 at 34.374 kb on + strand, within GFF1325 at 34.375 kb on - strand, within GFF1325 at 34.422 kb on + strand, within GFF1325 at 34.422 kb on + strand, within GFF1325 at 34.423 kb on - strand, within GFF1325 at 34.442 kb on + strand at 34.442 kb on + strand at 34.542 kb on - strand at 34.611 kb on + strand at 34.751 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 32,046 - GFF1323 0.43 +0.3 32,198 + GFF1323 0.55 +0.2 32,198 + GFF1323 0.55 +0.8 32,225 - GFF1323 0.57 -0.6 32,390 + GFF1323 0.70 -0.2 32,417 + GFF1323 0.72 +0.7 32,418 - GFF1323 0.72 -0.4 32,586 - GFF1323 0.86 +0.4 32,586 - GFF1323 0.86 -0.5 32,750 - +0.0 32,765 + -0.1 32,766 - +0.1 32,883 - +0.4 33,055 + GFF1324 0.18 +0.6 33,094 + GFF1324 0.23 +0.7 33,220 + GFF1324 0.36 +0.7 33,261 - GFF1324 0.40 +0.3 33,370 + GFF1324 0.52 +0.2 33,371 - GFF1324 0.52 +0.0 33,394 + GFF1324 0.55 -0.8 33,506 + GFF1324 0.67 +1.2 33,511 + GFF1324 0.67 +0.4 33,612 - GFF1324 0.78 -0.2 33,744 - -0.2 33,816 + +0.5 33,817 - +0.1 33,898 + GFF1325 0.15 -0.7 33,899 - GFF1325 0.15 +0.6 33,968 + GFF1325 0.25 +0.3 34,117 - GFF1325 0.46 +0.4 34,286 - GFF1325 0.70 +0.2 34,348 + GFF1325 0.79 +0.7 34,349 - GFF1325 0.79 -0.2 34,369 + GFF1325 0.82 -1.1 34,374 + GFF1325 0.82 -0.5 34,375 - GFF1325 0.83 -0.2 34,422 + GFF1325 0.89 +0.4 34,422 + GFF1325 0.89 -0.6 34,423 - GFF1325 0.89 +0.1 34,442 + +0.0 34,442 + +0.5 34,542 - +0.3 34,611 + -0.8 34,751 - +0.3
Or see this region's nucleotide sequence