Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1321 and GFF1322 are separated by 13 nucleotides GFF1322 and GFF1323 are separated by 9 nucleotides
GFF1321 - Galactose operon repressor, GalR-LacI family of transcriptional regulators, at 29,432 to 30,460
GFF1321
GFF1322 - Galactose operon repressor, GalR-LacI family of transcriptional regulators, at 30,474 to 31,493
GFF1322
GFF1323 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 31,503 to 32,765
GFF1323
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-1
0
1
2 at 29.498 kb on - strand at 29.723 kb on + strand, within GFF1321 at 29.883 kb on + strand, within GFF1321 at 29.973 kb on - strand, within GFF1321 at 29.975 kb on - strand, within GFF1321 at 30.161 kb on + strand, within GFF1321 at 30.161 kb on + strand, within GFF1321 at 30.195 kb on + strand, within GFF1321 at 30.196 kb on - strand, within GFF1321 at 30.205 kb on + strand, within GFF1321 at 30.232 kb on - strand, within GFF1321 at 30.255 kb on + strand, within GFF1321 at 30.343 kb on - strand, within GFF1321 at 30.375 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.467 kb on + strand at 30.467 kb on + strand at 30.468 kb on - strand at 30.770 kb on + strand, within GFF1322 at 31.023 kb on - strand, within GFF1322 at 31.023 kb on - strand, within GFF1322 at 31.037 kb on + strand, within GFF1322 at 31.060 kb on + strand, within GFF1322 at 31.132 kb on - strand, within GFF1322 at 31.198 kb on + strand, within GFF1322 at 31.199 kb on - strand, within GFF1322 at 31.208 kb on + strand, within GFF1322 at 31.258 kb on + strand, within GFF1322 at 31.259 kb on - strand, within GFF1322 at 31.345 kb on + strand, within GFF1322 at 31.610 kb on - strand at 31.610 kb on - strand at 31.633 kb on + strand, within GFF1323 at 31.654 kb on - strand, within GFF1323 at 31.654 kb on - strand, within GFF1323 at 31.817 kb on + strand, within GFF1323 at 31.826 kb on - strand, within GFF1323 at 31.826 kb on - strand, within GFF1323 at 31.830 kb on - strand, within GFF1323 at 31.834 kb on + strand, within GFF1323 at 32.046 kb on - strand, within GFF1323 at 32.198 kb on + strand, within GFF1323 at 32.198 kb on + strand, within GFF1323 at 32.225 kb on - strand, within GFF1323 at 32.390 kb on + strand, within GFF1323 at 32.417 kb on + strand, within GFF1323 at 32.418 kb on - strand, within GFF1323
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 29,498 - +0.2 29,723 + GFF1321 0.28 +0.8 29,883 + GFF1321 0.44 -0.5 29,973 - GFF1321 0.53 +0.3 29,975 - GFF1321 0.53 -0.5 30,161 + GFF1321 0.71 +0.6 30,161 + GFF1321 0.71 +0.8 30,195 + GFF1321 0.74 +0.3 30,196 - GFF1321 0.74 -0.7 30,205 + GFF1321 0.75 -0.5 30,232 - GFF1321 0.78 +0.6 30,255 + GFF1321 0.80 +0.3 30,343 - GFF1321 0.89 +0.5 30,375 + -0.0 30,384 + +0.5 30,384 + -0.1 30,384 + -0.6 30,384 + -1.6 30,467 + -0.6 30,467 + +0.9 30,468 - -0.3 30,770 + GFF1322 0.29 +0.4 31,023 - GFF1322 0.54 -0.0 31,023 - GFF1322 0.54 +0.2 31,037 + GFF1322 0.55 +0.5 31,060 + GFF1322 0.57 +0.7 31,132 - GFF1322 0.65 +0.0 31,198 + GFF1322 0.71 -0.1 31,199 - GFF1322 0.71 +0.0 31,208 + GFF1322 0.72 +0.7 31,258 + GFF1322 0.77 +0.6 31,259 - GFF1322 0.77 -0.4 31,345 + GFF1322 0.85 +1.5 31,610 - +0.6 31,610 - +2.5 31,633 + GFF1323 0.10 +0.5 31,654 - GFF1323 0.12 -0.4 31,654 - GFF1323 0.12 +0.4 31,817 + GFF1323 0.25 +0.4 31,826 - GFF1323 0.26 +0.0 31,826 - GFF1323 0.26 +0.5 31,830 - GFF1323 0.26 -0.1 31,834 + GFF1323 0.26 +0.4 32,046 - GFF1323 0.43 +0.3 32,198 + GFF1323 0.55 +0.2 32,198 + GFF1323 0.55 +0.8 32,225 - GFF1323 0.57 -0.6 32,390 + GFF1323 0.70 -0.2 32,417 + GFF1323 0.72 +0.7 32,418 - GFF1323 0.72 -0.4
Or see this region's nucleotide sequence