Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1030 and GFF1138 are separated by 31 nucleotides GFF1138 and GFF1139 are separated by 31 nucleotides GFF1139 and GFF1140 are separated by 27 nucleotides GFF1140 and GFF1031 are separated by 190 nucleotides GFF1031 and GFF1032 are separated by 102 nucleotides GFF1032 and GFF1033 are separated by 94 nucleotides GFF1033 and GFF1034 are separated by 21 nucleotides
GFF1030 - FIG00638078: hypothetical protein, at 213,713 to 213,949
GFF1030
GFF1138 - tRNA-Leu-CAG, at 213,981 to 214,067
GFF1138
GFF1139 - tRNA-Leu-CAG, at 214,099 to 214,185
GFF1139
GFF1140 - tRNA-Leu-CAG, at 214,213 to 214,299
GFF1140
GFF1031 - Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52), at 214,490 to 215,518
GFF1031
GFF1032 - DNA polymerase III psi subunit (EC 2.7.7.7), at 215,621 to 216,058
GFF1032
GFF1033 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 216,153 to 216,449
GFF1033
GFF1034 - '5'-nucleotidase YjjG (EC 3.1.3.5)' transl_table=11, at 216,471 to 217,148
GFF1034
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 213.600 kb on - strand at 213.748 kb on - strand, within GFF1030 at 213.750 kb on + strand, within GFF1030 at 213.896 kb on - strand, within GFF1030 at 213.973 kb on - strand at 213.978 kb on - strand at 214.369 kb on + strand at 214.374 kb on - strand at 214.479 kb on + strand at 214.480 kb on - strand at 214.525 kb on - strand at 214.772 kb on + strand, within GFF1031 at 214.995 kb on - strand, within GFF1031 at 215.117 kb on + strand, within GFF1031 at 215.118 kb on - strand, within GFF1031 at 215.118 kb on - strand, within GFF1031 at 215.227 kb on + strand, within GFF1031 at 215.257 kb on + strand, within GFF1031 at 215.271 kb on + strand, within GFF1031 at 215.272 kb on - strand, within GFF1031 at 215.274 kb on + strand, within GFF1031 at 215.275 kb on - strand, within GFF1031 at 215.324 kb on - strand, within GFF1031 at 215.357 kb on + strand, within GFF1031 at 215.383 kb on + strand, within GFF1031 at 215.384 kb on - strand, within GFF1031 at 215.384 kb on - strand, within GFF1031 at 215.552 kb on + strand at 215.553 kb on - strand at 215.553 kb on - strand at 215.555 kb on + strand at 215.556 kb on - strand at 215.588 kb on + strand at 215.659 kb on + strand at 215.662 kb on + strand at 215.757 kb on + strand, within GFF1032 at 215.876 kb on - strand, within GFF1032 at 216.131 kb on + strand at 216.132 kb on - strand at 216.132 kb on - strand at 216.170 kb on + strand at 216.248 kb on + strand, within GFF1033 at 216.296 kb on - strand, within GFF1033 at 216.296 kb on - strand, within GFF1033 at 216.314 kb on - strand, within GFF1033 at 216.345 kb on + strand, within GFF1033 at 216.387 kb on + strand, within GFF1033 at 216.440 kb on - strand at 216.448 kb on - strand at 216.467 kb on - strand at 216.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 213,600 - +0.1 213,748 - GFF1030 0.15 +0.1 213,750 + GFF1030 0.16 -0.0 213,896 - GFF1030 0.77 +1.1 213,973 - +0.4 213,978 - +0.4 214,369 + +0.6 214,374 - +0.2 214,479 + -0.6 214,480 - +0.6 214,525 - +1.3 214,772 + GFF1031 0.27 +0.2 214,995 - GFF1031 0.49 +1.9 215,117 + GFF1031 0.61 -1.5 215,118 - GFF1031 0.61 +0.5 215,118 - GFF1031 0.61 +0.9 215,227 + GFF1031 0.72 +2.8 215,257 + GFF1031 0.75 +1.4 215,271 + GFF1031 0.76 +1.8 215,272 - GFF1031 0.76 +2.5 215,274 + GFF1031 0.76 +0.6 215,275 - GFF1031 0.76 +0.4 215,324 - GFF1031 0.81 -0.1 215,357 + GFF1031 0.84 +0.3 215,383 + GFF1031 0.87 +0.3 215,384 - GFF1031 0.87 +0.1 215,384 - GFF1031 0.87 +0.2 215,552 + +0.4 215,553 - +0.1 215,553 - -1.1 215,555 + -0.2 215,556 - +0.7 215,588 + -0.8 215,659 + -1.2 215,662 + -1.8 215,757 + GFF1032 0.31 +1.4 215,876 - GFF1032 0.58 +1.2 216,131 + -1.1 216,132 - -0.5 216,132 - +0.4 216,170 + -1.2 216,248 + GFF1033 0.32 -1.3 216,296 - GFF1033 0.48 +0.7 216,296 - GFF1033 0.48 -1.9 216,314 - GFF1033 0.54 -0.8 216,345 + GFF1033 0.65 -0.8 216,387 + GFF1033 0.79 +0.4 216,440 - -0.6 216,448 - +1.3 216,467 - -0.0 216,516 - +1.1
Or see this region's nucleotide sequence