Experiment: Vancomycin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1231 and PfGW456L13_1232 are separated by 33 nucleotides PfGW456L13_1232 and PfGW456L13_1233 are separated by 135 nucleotides PfGW456L13_1233 and PfGW456L13_1234 are separated by 166 nucleotides PfGW456L13_1234 and PfGW456L13_1235 are separated by 68 nucleotides
PfGW456L13_1231 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 1,372,367 to 1,373,335
_1231
PfGW456L13_1232 - Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-), at 1,373,369 to 1,373,797
_1232
PfGW456L13_1233 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 1,373,933 to 1,374,967
_1233
PfGW456L13_1234 - probable iron binding protein from the HesB_IscA_SufA family, at 1,375,134 to 1,375,484
_1234
PfGW456L13_1235 - Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-), at 1,375,553 to 1,376,644
_1235
Position (kb)
1373
1374
1375 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1372.986 kb on + strand, within PfGW456L13_1231 at 1372.987 kb on - strand, within PfGW456L13_1231 at 1373.081 kb on + strand, within PfGW456L13_1231 at 1373.081 kb on + strand, within PfGW456L13_1231 at 1373.121 kb on + strand, within PfGW456L13_1231 at 1373.121 kb on + strand, within PfGW456L13_1231 at 1373.121 kb on + strand, within PfGW456L13_1231 at 1373.121 kb on + strand, within PfGW456L13_1231 at 1373.250 kb on - strand at 1373.370 kb on + strand at 1373.371 kb on - strand at 1373.822 kb on + strand at 1373.832 kb on - strand at 1373.832 kb on - strand at 1373.832 kb on - strand at 1373.832 kb on - strand at 1373.832 kb on - strand at 1373.834 kb on + strand at 1373.834 kb on + strand at 1373.835 kb on - strand at 1373.891 kb on + strand at 1373.892 kb on - strand at 1373.894 kb on + strand at 1373.933 kb on - strand at 1373.933 kb on - strand at 1373.933 kb on - strand at 1373.966 kb on + strand at 1374.068 kb on + strand, within PfGW456L13_1233 at 1374.068 kb on + strand, within PfGW456L13_1233 at 1374.069 kb on - strand, within PfGW456L13_1233 at 1374.069 kb on - strand, within PfGW456L13_1233 at 1374.069 kb on - strand, within PfGW456L13_1233 at 1374.069 kb on - strand, within PfGW456L13_1233 at 1374.089 kb on + strand, within PfGW456L13_1233 at 1374.317 kb on + strand, within PfGW456L13_1233 at 1374.513 kb on - strand, within PfGW456L13_1233 at 1374.542 kb on + strand, within PfGW456L13_1233 at 1374.542 kb on + strand, within PfGW456L13_1233 at 1374.543 kb on - strand, within PfGW456L13_1233 at 1375.041 kb on + strand at 1375.041 kb on + strand at 1375.041 kb on + strand at 1375.042 kb on - strand at 1375.042 kb on - strand at 1375.042 kb on - strand at 1375.043 kb on + strand at 1375.043 kb on + strand at 1375.043 kb on + strand at 1375.043 kb on + strand at 1375.043 kb on + strand at 1375.043 kb on + strand at 1375.043 kb on + strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.044 kb on - strand at 1375.054 kb on + strand at 1375.070 kb on + strand at 1375.103 kb on + strand at 1375.103 kb on + strand at 1375.103 kb on + strand at 1375.103 kb on + strand at 1375.103 kb on + strand at 1375.113 kb on + strand at 1375.554 kb on + strand at 1375.554 kb on + strand at 1375.555 kb on - strand at 1375.555 kb on - strand at 1375.563 kb on + strand at 1375.563 kb on + strand at 1375.693 kb on - strand, within PfGW456L13_1235 at 1375.693 kb on - strand, within PfGW456L13_1235 at 1375.693 kb on - strand, within PfGW456L13_1235 at 1375.862 kb on + strand, within PfGW456L13_1235 at 1375.862 kb on + strand, within PfGW456L13_1235 at 1375.862 kb on + strand, within PfGW456L13_1235 at 1375.863 kb on - strand, within PfGW456L13_1235 at 1375.863 kb on - strand, within PfGW456L13_1235 at 1375.863 kb on - strand, within PfGW456L13_1235
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.125 mg/ml remove 1,372,986 + PfGW456L13_1231 0.64 -1.0 1,372,987 - PfGW456L13_1231 0.64 -1.6 1,373,081 + PfGW456L13_1231 0.74 +0.3 1,373,081 + PfGW456L13_1231 0.74 -0.1 1,373,121 + PfGW456L13_1231 0.78 +0.7 1,373,121 + PfGW456L13_1231 0.78 +0.7 1,373,121 + PfGW456L13_1231 0.78 +1.6 1,373,121 + PfGW456L13_1231 0.78 -0.6 1,373,250 - -2.2 1,373,370 + -0.1 1,373,371 - -1.6 1,373,822 + -0.4 1,373,832 - +0.8 1,373,832 - -0.6 1,373,832 - +0.8 1,373,832 - +0.4 1,373,832 - -0.2 1,373,834 + -0.1 1,373,834 + +0.7 1,373,835 - -0.4 1,373,891 + +1.3 1,373,892 - -0.4 1,373,894 + -1.5 1,373,933 - -1.8 1,373,933 - -2.4 1,373,933 - -1.6 1,373,966 + -2.3 1,374,068 + PfGW456L13_1233 0.13 -0.4 1,374,068 + PfGW456L13_1233 0.13 -3.8 1,374,069 - PfGW456L13_1233 0.13 -3.1 1,374,069 - PfGW456L13_1233 0.13 +0.8 1,374,069 - PfGW456L13_1233 0.13 -2.3 1,374,069 - PfGW456L13_1233 0.13 -3.6 1,374,089 + PfGW456L13_1233 0.15 -1.8 1,374,317 + PfGW456L13_1233 0.37 -0.8 1,374,513 - PfGW456L13_1233 0.56 -1.8 1,374,542 + PfGW456L13_1233 0.59 -1.5 1,374,542 + PfGW456L13_1233 0.59 -1.5 1,374,543 - PfGW456L13_1233 0.59 -0.5 1,375,041 + -0.0 1,375,041 + -0.0 1,375,041 + +0.4 1,375,042 - -0.6 1,375,042 - -0.4 1,375,042 - +0.3 1,375,043 + -0.9 1,375,043 + +0.6 1,375,043 + +0.8 1,375,043 + +0.3 1,375,043 + -1.6 1,375,043 + +0.8 1,375,043 + +0.2 1,375,044 - +0.2 1,375,044 - -0.5 1,375,044 - +0.0 1,375,044 - -0.1 1,375,044 - +0.6 1,375,044 - -0.7 1,375,044 - +0.5 1,375,044 - -0.7 1,375,044 - -0.8 1,375,044 - +0.5 1,375,044 - +0.2 1,375,054 + +0.8 1,375,070 + -1.0 1,375,103 + +0.2 1,375,103 + +1.3 1,375,103 + +0.3 1,375,103 + +0.5 1,375,103 + +0.4 1,375,113 + -0.1 1,375,554 + +0.2 1,375,554 + -0.3 1,375,555 - +0.6 1,375,555 - -0.5 1,375,563 + +1.8 1,375,563 + +0.4 1,375,693 - PfGW456L13_1235 0.13 +1.7 1,375,693 - PfGW456L13_1235 0.13 +1.6 1,375,693 - PfGW456L13_1235 0.13 +2.2 1,375,862 + PfGW456L13_1235 0.28 +1.8 1,375,862 + PfGW456L13_1235 0.28 +1.7 1,375,862 + PfGW456L13_1235 0.28 +1.1 1,375,863 - PfGW456L13_1235 0.28 +1.4 1,375,863 - PfGW456L13_1235 0.28 +0.5 1,375,863 - PfGW456L13_1235 0.28 +0.2
Or see this region's nucleotide sequence