Experiment: m.b. Spectinomycin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2266 and B158DRAFT_2267 are separated by 97 nucleotides B158DRAFT_2267 and B158DRAFT_2268 are separated by 92 nucleotides
B158DRAFT_2266: B158DRAFT_2266 - Protein-disulfide isomerase, at 428,734 to 429,492
_2266
B158DRAFT_2267: B158DRAFT_2267 - Uncharacterized conserved protein, at 429,590 to 429,958
_2267
B158DRAFT_2268: B158DRAFT_2268 - single-stranded-DNA-specific exonuclease RecJ, at 430,051 to 431,778
_2268
Position (kb)
429
430 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 428.607 kb on + strand at 428.801 kb on - strand at 428.868 kb on + strand, within B158DRAFT_2266 at 428.889 kb on + strand, within B158DRAFT_2266 at 429.070 kb on + strand, within B158DRAFT_2266 at 429.234 kb on - strand, within B158DRAFT_2266 at 429.252 kb on + strand, within B158DRAFT_2266 at 429.267 kb on - strand, within B158DRAFT_2266 at 429.352 kb on + strand, within B158DRAFT_2266 at 429.469 kb on - strand at 429.479 kb on - strand at 429.505 kb on - strand at 429.516 kb on - strand at 429.535 kb on + strand at 429.538 kb on - strand at 429.594 kb on + strand at 429.594 kb on + strand at 429.596 kb on + strand at 429.597 kb on - strand at 429.600 kb on + strand at 429.602 kb on - strand at 429.607 kb on - strand at 429.613 kb on - strand at 429.618 kb on - strand at 429.639 kb on + strand, within B158DRAFT_2267 at 429.663 kb on - strand, within B158DRAFT_2267 at 429.671 kb on - strand, within B158DRAFT_2267 at 429.700 kb on - strand, within B158DRAFT_2267 at 429.733 kb on + strand, within B158DRAFT_2267 at 429.784 kb on + strand, within B158DRAFT_2267 at 429.784 kb on - strand, within B158DRAFT_2267 at 429.834 kb on + strand, within B158DRAFT_2267 at 429.924 kb on - strand at 429.935 kb on + strand at 429.936 kb on + strand at 429.936 kb on + strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.974 kb on + strand at 429.974 kb on + strand at 429.974 kb on + strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 430.099 kb on + strand at 430.103 kb on + strand at 430.133 kb on - strand at 430.133 kb on - strand at 430.133 kb on - strand at 430.142 kb on + strand at 430.142 kb on + strand at 430.142 kb on + strand at 430.150 kb on - strand at 430.150 kb on - strand at 430.150 kb on - strand at 430.159 kb on + strand at 430.219 kb on + strand at 430.389 kb on - strand, within B158DRAFT_2268 at 430.395 kb on - strand, within B158DRAFT_2268 at 430.422 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.484 kb on - strand, within B158DRAFT_2268 at 430.532 kb on - strand, within B158DRAFT_2268 at 430.671 kb on - strand, within B158DRAFT_2268 at 430.688 kb on + strand, within B158DRAFT_2268 at 430.688 kb on + strand, within B158DRAFT_2268 at 430.696 kb on - strand, within B158DRAFT_2268 at 430.696 kb on - strand, within B158DRAFT_2268 at 430.696 kb on - strand, within B158DRAFT_2268 at 430.757 kb on + strand, within B158DRAFT_2268 at 430.803 kb on + strand, within B158DRAFT_2268 at 430.811 kb on - strand, within B158DRAFT_2268 at 430.811 kb on - strand, within B158DRAFT_2268 at 430.813 kb on + strand, within B158DRAFT_2268 at 430.813 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.911 kb on + strand, within B158DRAFT_2268 at 430.919 kb on - strand, within B158DRAFT_2268 at 430.919 kb on - strand, within B158DRAFT_2268 at 430.936 kb on + strand, within B158DRAFT_2268
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.1 mg/ml remove 428,607 + -0.1 428,801 - -1.0 428,868 + B158DRAFT_2266 0.18 -0.9 428,889 + B158DRAFT_2266 0.20 -0.6 429,070 + B158DRAFT_2266 0.44 -0.2 429,234 - B158DRAFT_2266 0.66 -0.3 429,252 + B158DRAFT_2266 0.68 -0.7 429,267 - B158DRAFT_2266 0.70 -1.1 429,352 + B158DRAFT_2266 0.81 -1.5 429,469 - -1.6 429,479 - -0.6 429,505 - -0.1 429,516 - -0.4 429,535 + -0.2 429,538 - +0.7 429,594 + -1.3 429,594 + -0.3 429,596 + -0.3 429,597 - +0.3 429,600 + -0.8 429,602 - +0.2 429,607 - -0.3 429,613 - -0.5 429,618 - -0.9 429,639 + B158DRAFT_2267 0.13 -0.5 429,663 - B158DRAFT_2267 0.20 -0.5 429,671 - B158DRAFT_2267 0.22 +0.1 429,700 - B158DRAFT_2267 0.30 -0.5 429,733 + B158DRAFT_2267 0.39 -0.2 429,784 + B158DRAFT_2267 0.53 -0.6 429,784 - B158DRAFT_2267 0.53 -0.7 429,834 + B158DRAFT_2267 0.66 -0.1 429,924 - +0.3 429,935 + -0.7 429,936 + -0.4 429,936 + -0.3 429,944 - +0.2 429,944 - +0.3 429,944 - +0.5 429,944 - -0.2 429,974 + -0.3 429,974 + -0.2 429,974 + -0.8 429,982 - -0.2 429,982 - -0.3 429,982 - -0.0 429,982 - -0.2 429,982 - +0.2 429,982 - -0.6 430,099 + -1.6 430,103 + -1.1 430,133 - -2.0 430,133 - -2.2 430,133 - -1.8 430,142 + -0.8 430,142 + +0.9 430,142 + -1.0 430,150 - -1.0 430,150 - -0.5 430,150 - -1.7 430,159 + -2.5 430,219 + -0.9 430,389 - B158DRAFT_2268 0.20 -1.4 430,395 - B158DRAFT_2268 0.20 -2.3 430,422 + B158DRAFT_2268 0.21 -2.0 430,476 + B158DRAFT_2268 0.25 -1.8 430,476 + B158DRAFT_2268 0.25 -1.2 430,476 + B158DRAFT_2268 0.25 -1.5 430,484 - B158DRAFT_2268 0.25 -1.9 430,532 - B158DRAFT_2268 0.28 -2.3 430,671 - B158DRAFT_2268 0.36 -2.0 430,688 + B158DRAFT_2268 0.37 -1.8 430,688 + B158DRAFT_2268 0.37 -1.6 430,696 - B158DRAFT_2268 0.37 -0.0 430,696 - B158DRAFT_2268 0.37 -1.8 430,696 - B158DRAFT_2268 0.37 -3.7 430,757 + B158DRAFT_2268 0.41 -1.7 430,803 + B158DRAFT_2268 0.44 -1.1 430,811 - B158DRAFT_2268 0.44 -1.0 430,811 - B158DRAFT_2268 0.44 -1.8 430,813 + B158DRAFT_2268 0.44 +0.1 430,813 + B158DRAFT_2268 0.44 -1.5 430,885 + B158DRAFT_2268 0.48 -0.9 430,885 + B158DRAFT_2268 0.48 -2.3 430,885 + B158DRAFT_2268 0.48 -3.1 430,885 + B158DRAFT_2268 0.48 -1.0 430,885 + B158DRAFT_2268 0.48 -1.8 430,885 + B158DRAFT_2268 0.48 -0.9 430,885 + B158DRAFT_2268 0.48 -1.7 430,885 + B158DRAFT_2268 0.48 -1.8 430,885 + B158DRAFT_2268 0.48 +0.2 430,893 - B158DRAFT_2268 0.49 -1.4 430,893 - B158DRAFT_2268 0.49 -1.5 430,893 - B158DRAFT_2268 0.49 -1.0 430,893 - B158DRAFT_2268 0.49 -1.4 430,893 - B158DRAFT_2268 0.49 -1.3 430,911 + B158DRAFT_2268 0.50 -1.8 430,919 - B158DRAFT_2268 0.50 -1.0 430,919 - B158DRAFT_2268 0.50 -1.4 430,936 + B158DRAFT_2268 0.51 -1.2
Or see this region's nucleotide sequence