Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF94 and GFF95 are separated by 61 nucleotides GFF95 and GFF96 are separated by 370 nucleotides GFF96 and GFF97 are separated by 47 nucleotides
GFF94 - Polysaccharide deacetylase, at 78,066 to 79,295
GFF94
GFF95 - Putative PTS system IIA component yadI (EC 2.7.1.69), at 79,357 to 79,797
GFF95
GFF96 - Cell wall surface anchor family protein, at 80,168 to 80,707
GFF96
GFF97 - Chaperone protein EcpD, at 80,755 to 81,438
GFF97
Position (kb)
79
80 Strain fitness (log2 ratio)
-2
-1
0
1 at 78.625 kb on + strand, within GFF94 at 78.625 kb on + strand, within GFF94 at 78.625 kb on + strand, within GFF94 at 78.625 kb on + strand, within GFF94 at 78.632 kb on - strand, within GFF94 at 78.632 kb on - strand, within GFF94 at 78.632 kb on - strand, within GFF94 at 78.646 kb on + strand, within GFF94 at 78.759 kb on + strand, within GFF94 at 78.773 kb on - strand, within GFF94 at 78.823 kb on + strand, within GFF94 at 78.823 kb on + strand, within GFF94 at 78.867 kb on + strand, within GFF94 at 79.106 kb on - strand, within GFF94 at 79.108 kb on + strand, within GFF94 at 79.222 kb on + strand at 79.275 kb on - strand at 79.295 kb on + strand at 79.296 kb on - strand at 79.296 kb on - strand at 79.296 kb on - strand at 79.296 kb on - strand at 79.297 kb on + strand at 79.385 kb on - strand at 79.672 kb on - strand, within GFF95 at 79.676 kb on - strand, within GFF95 at 79.676 kb on - strand, within GFF95 at 80.259 kb on + strand, within GFF96 at 80.260 kb on - strand, within GFF96 at 80.263 kb on + strand, within GFF96 at 80.633 kb on + strand, within GFF96 at 80.702 kb on - strand at 80.702 kb on - strand at 80.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 78,625 + GFF94 0.45 -0.2 78,625 + GFF94 0.45 -1.0 78,625 + GFF94 0.45 -0.5 78,625 + GFF94 0.45 +0.1 78,632 - GFF94 0.46 +0.0 78,632 - GFF94 0.46 +0.5 78,632 - GFF94 0.46 -0.1 78,646 + GFF94 0.47 -0.1 78,759 + GFF94 0.56 +0.2 78,773 - GFF94 0.57 +0.3 78,823 + GFF94 0.62 -1.2 78,823 + GFF94 0.62 -0.7 78,867 + GFF94 0.65 -0.5 79,106 - GFF94 0.85 -0.8 79,108 + GFF94 0.85 -0.4 79,222 + -0.6 79,275 - +0.0 79,295 + -0.5 79,296 - +0.2 79,296 - +0.1 79,296 - -0.2 79,296 - -2.3 79,297 + +0.1 79,385 - +0.2 79,672 - GFF95 0.71 +0.1 79,676 - GFF95 0.72 -1.6 79,676 - GFF95 0.72 -0.2 80,259 + GFF96 0.17 +1.1 80,260 - GFF96 0.17 -1.2 80,263 + GFF96 0.18 +0.1 80,633 + GFF96 0.86 +0.0 80,702 - +0.3 80,702 - +0.5 80,702 - +0.3
Or see this region's nucleotide sequence