Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF883 and GFF884 are separated by 50 nucleotides GFF884 and GFF885 are separated by 242 nucleotides GFF885 and GFF886 are separated by 36 nucleotides
GFF883 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 61,890 to 62,723
GFF883
GFF884 - Major myo-inositol transporter IolT, at 62,774 to 64,207
GFF884
GFF885 - FIG01047800: hypothetical protein, at 64,450 to 64,629
GFF885
GFF886 - Major myo-inositol transporter IolT, at 64,666 to 66,102
GFF886
Position (kb) 62
63
64
65 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 61.794 kb on - strand at 61.849 kb on - strand at 61.895 kb on + strand at 61.983 kb on + strand, within GFF883 at 62.000 kb on + strand, within GFF883 at 62.000 kb on + strand, within GFF883 at 62.001 kb on - strand, within GFF883 at 62.003 kb on + strand, within GFF883 at 62.004 kb on - strand, within GFF883 at 62.023 kb on + strand, within GFF883 at 62.103 kb on - strand, within GFF883 at 62.154 kb on + strand, within GFF883 at 62.263 kb on + strand, within GFF883 at 62.343 kb on + strand, within GFF883 at 62.383 kb on - strand, within GFF883 at 62.461 kb on + strand, within GFF883 at 62.465 kb on + strand, within GFF883 at 62.465 kb on + strand, within GFF883 at 62.511 kb on + strand, within GFF883 at 62.533 kb on + strand, within GFF883 at 62.835 kb on - strand at 62.881 kb on - strand at 63.003 kb on - strand, within GFF884 at 63.022 kb on + strand, within GFF884 at 63.082 kb on + strand, within GFF884 at 63.174 kb on + strand, within GFF884 at 63.373 kb on - strand, within GFF884 at 63.373 kb on - strand, within GFF884 at 63.373 kb on - strand, within GFF884 at 63.373 kb on - strand, within GFF884 at 63.373 kb on - strand, within GFF884 at 63.395 kb on + strand, within GFF884 at 63.396 kb on - strand, within GFF884 at 63.396 kb on - strand, within GFF884 at 63.396 kb on - strand, within GFF884 at 63.482 kb on + strand, within GFF884 at 63.483 kb on - strand, within GFF884 at 63.630 kb on - strand, within GFF884 at 63.655 kb on + strand, within GFF884 at 63.673 kb on + strand, within GFF884 at 63.673 kb on + strand, within GFF884 at 63.674 kb on - strand, within GFF884 at 63.674 kb on - strand, within GFF884 at 63.675 kb on + strand, within GFF884 at 63.828 kb on + strand, within GFF884 at 63.829 kb on - strand, within GFF884 at 63.942 kb on - strand, within GFF884 at 64.077 kb on + strand at 64.108 kb on + strand at 64.169 kb on + strand at 64.169 kb on + strand at 64.169 kb on + strand at 64.174 kb on - strand at 64.185 kb on + strand at 64.186 kb on - strand at 64.192 kb on + strand at 64.193 kb on - strand at 64.208 kb on - strand at 64.316 kb on - strand at 64.396 kb on - strand at 64.414 kb on + strand at 64.665 kb on + strand at 64.716 kb on + strand at 64.784 kb on + strand at 64.852 kb on - strand, within GFF886 at 65.041 kb on + strand, within GFF886 at 65.041 kb on + strand, within GFF886 at 65.041 kb on + strand, within GFF886 at 65.041 kb on + strand, within GFF886 at 65.042 kb on - strand, within GFF886 at 65.042 kb on - strand, within GFF886 at 65.042 kb on - strand, within GFF886 at 65.042 kb on - strand, within GFF886 at 65.042 kb on - strand, within GFF886 at 65.042 kb on - strand, within GFF886 at 65.043 kb on + strand, within GFF886 at 65.044 kb on - strand, within GFF886 at 65.092 kb on + strand, within GFF886 at 65.197 kb on - strand, within GFF886 at 65.197 kb on - strand, within GFF886
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 61,794 - -0.8 61,849 - -0.0 61,895 + +1.0 61,983 + GFF883 0.11 -0.2 62,000 + GFF883 0.13 -0.6 62,000 + GFF883 0.13 +0.4 62,001 - GFF883 0.13 -1.1 62,003 + GFF883 0.14 -0.6 62,004 - GFF883 0.14 +1.0 62,023 + GFF883 0.16 +1.2 62,103 - GFF883 0.26 +1.0 62,154 + GFF883 0.32 -0.1 62,263 + GFF883 0.45 -0.9 62,343 + GFF883 0.54 -0.2 62,383 - GFF883 0.59 +1.4 62,461 + GFF883 0.68 -0.3 62,465 + GFF883 0.69 +0.9 62,465 + GFF883 0.69 -0.1 62,511 + GFF883 0.74 +0.2 62,533 + GFF883 0.77 -0.6 62,835 - -0.0 62,881 - +0.4 63,003 - GFF884 0.16 +0.7 63,022 + GFF884 0.17 +0.6 63,082 + GFF884 0.21 +0.4 63,174 + GFF884 0.28 +0.6 63,373 - GFF884 0.42 +0.5 63,373 - GFF884 0.42 -2.2 63,373 - GFF884 0.42 -0.1 63,373 - GFF884 0.42 +0.4 63,373 - GFF884 0.42 -2.2 63,395 + GFF884 0.43 +0.6 63,396 - GFF884 0.43 +0.8 63,396 - GFF884 0.43 -0.2 63,396 - GFF884 0.43 -0.2 63,482 + GFF884 0.49 -0.1 63,483 - GFF884 0.49 +0.2 63,630 - GFF884 0.60 -0.6 63,655 + GFF884 0.61 +1.1 63,673 + GFF884 0.63 +0.4 63,673 + GFF884 0.63 +0.4 63,674 - GFF884 0.63 -0.6 63,674 - GFF884 0.63 -0.2 63,675 + GFF884 0.63 +1.3 63,828 + GFF884 0.74 -0.5 63,829 - GFF884 0.74 +0.4 63,942 - GFF884 0.81 -0.2 64,077 + -0.7 64,108 + -1.9 64,169 + -1.1 64,169 + -0.9 64,169 + +0.2 64,174 - +0.3 64,185 + -0.7 64,186 - +0.3 64,192 + +0.2 64,193 - -0.9 64,208 - -0.7 64,316 - -0.2 64,396 - -0.9 64,414 + -0.9 64,665 + -0.4 64,716 + -0.4 64,784 + +0.2 64,852 - GFF886 0.13 +1.6 65,041 + GFF886 0.26 +1.2 65,041 + GFF886 0.26 +0.6 65,041 + GFF886 0.26 -1.6 65,041 + GFF886 0.26 -1.7 65,042 - GFF886 0.26 -1.2 65,042 - GFF886 0.26 -0.0 65,042 - GFF886 0.26 -1.2 65,042 - GFF886 0.26 +0.2 65,042 - GFF886 0.26 -1.3 65,042 - GFF886 0.26 +0.9 65,043 + GFF886 0.26 +0.2 65,044 - GFF886 0.26 -0.6 65,092 + GFF886 0.30 -2.0 65,197 - GFF886 0.37 -0.3 65,197 - GFF886 0.37 +2.8
Or see this region's nucleotide sequence