Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF824 and GFF825 are separated by 82 nucleotides GFF825 and GFF826 are separated by 71 nucleotides GFF826 and GFF827 are separated by 214 nucleotides
GFF824 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 6,937 to 7,842
GFF824
GFF825 - RNA-binding protein Hfq, at 7,925 to 8,233
GFF825
GFF826 - GTP-binding protein HflX, at 8,305 to 9,585
GFF826
GFF827 - HflK protein, at 9,800 to 11,059
GFF827
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 7.347 kb on - strand, within GFF824 at 7.660 kb on + strand, within GFF824 at 7.660 kb on + strand, within GFF824 at 7.681 kb on + strand, within GFF824 at 7.682 kb on - strand, within GFF824 at 7.686 kb on + strand, within GFF824 at 7.811 kb on + strand at 7.814 kb on + strand at 7.849 kb on - strand at 7.912 kb on + strand at 8.015 kb on - strand, within GFF825 at 8.089 kb on + strand, within GFF825 at 8.167 kb on + strand, within GFF825 at 8.168 kb on - strand, within GFF825 at 8.171 kb on + strand, within GFF825 at 8.232 kb on - strand at 8.290 kb on + strand at 8.290 kb on + strand at 8.291 kb on - strand at 8.291 kb on - strand at 8.354 kb on - strand at 8.487 kb on + strand, within GFF826 at 8.987 kb on + strand, within GFF826 at 8.987 kb on + strand, within GFF826 at 9.012 kb on - strand, within GFF826 at 9.243 kb on - strand, within GFF826 at 9.515 kb on + strand at 9.584 kb on - strand at 9.606 kb on + strand at 9.731 kb on + strand at 9.928 kb on + strand, within GFF827 at 10.063 kb on - strand, within GFF827 at 10.390 kb on + strand, within GFF827 at 10.394 kb on + strand, within GFF827 at 10.468 kb on + strand, within GFF827
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 7,347 - GFF824 0.45 +2.1 7,660 + GFF824 0.80 +4.3 7,660 + GFF824 0.80 +2.4 7,681 + GFF824 0.82 +1.9 7,682 - GFF824 0.82 +2.6 7,686 + GFF824 0.83 +2.6 7,811 + -0.2 7,814 + -1.3 7,849 - -1.2 7,912 + -0.6 8,015 - GFF825 0.29 -1.4 8,089 + GFF825 0.53 -1.1 8,167 + GFF825 0.78 +3.0 8,168 - GFF825 0.79 -2.8 8,171 + GFF825 0.80 -2.2 8,232 - -1.8 8,290 + -0.4 8,290 + -1.2 8,291 - -1.5 8,291 - -0.3 8,354 - -0.9 8,487 + GFF826 0.14 -0.9 8,987 + GFF826 0.53 -0.2 8,987 + GFF826 0.53 -0.7 9,012 - GFF826 0.55 -1.2 9,243 - GFF826 0.73 -0.7 9,515 + +0.1 9,584 - -1.2 9,606 + -1.0 9,731 + -0.3 9,928 + GFF827 0.10 +0.1 10,063 - GFF827 0.21 +0.4 10,390 + GFF827 0.47 -0.6 10,394 + GFF827 0.47 -1.1 10,468 + GFF827 0.53 -0.8
Or see this region's nucleotide sequence