Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF715 and GFF716 are separated by 43 nucleotides GFF716 and GFF717 are separated by 218 nucleotides
GFF715 - Putative membrane protein, at 69,805 to 70,917
GFF715
GFF716 - L,D-transpeptidase YbiS, at 70,961 to 71,881
GFF716
GFF717 - Putative ATPase component of ABC transporter with duplicated ATPase domain, at 72,100 to 73,692
GFF717
Position (kb) 70
71
72 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 70.365 kb on - strand, within GFF715 at 70.368 kb on + strand, within GFF715 at 70.374 kb on + strand, within GFF715 at 70.430 kb on + strand, within GFF715 at 70.430 kb on + strand, within GFF715 at 70.432 kb on + strand, within GFF715 at 70.433 kb on - strand, within GFF715 at 70.433 kb on - strand, within GFF715 at 70.560 kb on + strand, within GFF715 at 70.573 kb on - strand, within GFF715 at 70.721 kb on - strand, within GFF715 at 70.721 kb on - strand, within GFF715 at 70.801 kb on - strand, within GFF715 at 70.801 kb on - strand, within GFF715 at 70.808 kb on + strand at 70.852 kb on + strand at 70.898 kb on + strand at 70.932 kb on + strand at 70.932 kb on + strand at 70.933 kb on - strand at 71.010 kb on - strand at 71.054 kb on - strand, within GFF716 at 71.059 kb on + strand, within GFF716 at 71.063 kb on - strand, within GFF716 at 71.136 kb on + strand, within GFF716 at 71.250 kb on - strand, within GFF716 at 71.310 kb on + strand, within GFF716 at 71.311 kb on - strand, within GFF716 at 71.488 kb on + strand, within GFF716 at 71.547 kb on + strand, within GFF716 at 71.550 kb on + strand, within GFF716 at 71.551 kb on - strand, within GFF716 at 71.734 kb on + strand, within GFF716 at 71.911 kb on - strand at 71.926 kb on - strand at 71.948 kb on + strand at 72.062 kb on - strand at 72.065 kb on - strand at 72.093 kb on + strand at 72.138 kb on + strand at 72.407 kb on - strand, within GFF717 at 72.442 kb on - strand, within GFF717 at 72.461 kb on + strand, within GFF717 at 72.462 kb on - strand, within GFF717 at 72.585 kb on - strand, within GFF717 at 72.608 kb on + strand, within GFF717 at 72.615 kb on + strand, within GFF717 at 72.667 kb on - strand, within GFF717 at 72.669 kb on + strand, within GFF717 at 72.756 kb on + strand, within GFF717 at 72.808 kb on + strand, within GFF717 at 72.808 kb on + strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.817 kb on + strand, within GFF717 at 72.818 kb on - strand, within GFF717 at 72.818 kb on - strand, within GFF717 at 72.818 kb on - strand, within GFF717
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 70,365 - GFF715 0.50 +0.4 70,368 + GFF715 0.51 -0.1 70,374 + GFF715 0.51 +1.3 70,430 + GFF715 0.56 -0.0 70,430 + GFF715 0.56 +1.0 70,432 + GFF715 0.56 +0.2 70,433 - GFF715 0.56 +0.4 70,433 - GFF715 0.56 -0.1 70,560 + GFF715 0.68 +0.7 70,573 - GFF715 0.69 +1.3 70,721 - GFF715 0.82 -0.1 70,721 - GFF715 0.82 +1.0 70,801 - GFF715 0.89 -1.2 70,801 - GFF715 0.89 +1.7 70,808 + +1.0 70,852 + -1.1 70,898 + +1.0 70,932 + +0.0 70,932 + -0.1 70,933 - +2.2 71,010 - +0.2 71,054 - GFF716 0.10 -0.7 71,059 + GFF716 0.11 -1.5 71,063 - GFF716 0.11 -0.9 71,136 + GFF716 0.19 +0.1 71,250 - GFF716 0.31 -2.4 71,310 + GFF716 0.38 -2.5 71,311 - GFF716 0.38 +0.3 71,488 + GFF716 0.57 +0.2 71,547 + GFF716 0.64 +0.5 71,550 + GFF716 0.64 +0.7 71,551 - GFF716 0.64 -1.5 71,734 + GFF716 0.84 -1.7 71,911 - -0.1 71,926 - +1.5 71,948 + +0.3 72,062 - +0.1 72,065 - -1.5 72,093 + -0.2 72,138 + -0.7 72,407 - GFF717 0.19 +0.0 72,442 - GFF717 0.21 +0.5 72,461 + GFF717 0.23 -0.9 72,462 - GFF717 0.23 -4.3 72,585 - GFF717 0.30 +2.1 72,608 + GFF717 0.32 -1.8 72,615 + GFF717 0.32 -0.7 72,667 - GFF717 0.36 +0.5 72,669 + GFF717 0.36 -0.1 72,756 + GFF717 0.41 -0.9 72,808 + GFF717 0.44 +0.7 72,808 + GFF717 0.44 +0.2 72,809 - GFF717 0.45 +0.9 72,809 - GFF717 0.45 +0.1 72,809 - GFF717 0.45 +1.7 72,809 - GFF717 0.45 -0.2 72,817 + GFF717 0.45 -0.8 72,818 - GFF717 0.45 -0.3 72,818 - GFF717 0.45 +0.9 72,818 - GFF717 0.45 -1.9
Or see this region's nucleotide sequence