Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4754 and GFF4755 are separated by 3 nucleotides GFF4755 and GFF4756 are separated by 25 nucleotides GFF4756 and GFF4757 overlap by 11 nucleotides GFF4757 and GFF4758 overlap by 1 nucleotides
GFF4754 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 62,373 to 63,164
GFF4754
GFF4755 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 63,168 to 63,428
GFF4755
GFF4756 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 63,454 to 64,128
GFF4756
GFF4757 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components); Surface presentation of antigens protein SpaO, at 64,118 to 65,029
GFF4757
GFF4758 - Type III secretion host injection and negative regulator protein (YopD), at 65,029 to 66,039
GFF4758
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1 at 62.491 kb on - strand, within GFF4754 at 62.596 kb on - strand, within GFF4754 at 62.712 kb on - strand, within GFF4754 at 62.774 kb on + strand, within GFF4754 at 62.801 kb on + strand, within GFF4754 at 62.828 kb on + strand, within GFF4754 at 62.829 kb on - strand, within GFF4754 at 62.831 kb on + strand, within GFF4754 at 62.864 kb on + strand, within GFF4754 at 62.947 kb on - strand, within GFF4754 at 63.230 kb on - strand, within GFF4755 at 63.325 kb on - strand, within GFF4755 at 63.391 kb on + strand, within GFF4755 at 63.401 kb on + strand, within GFF4755 at 63.478 kb on + strand at 63.538 kb on - strand, within GFF4756 at 63.546 kb on + strand, within GFF4756 at 63.547 kb on - strand, within GFF4756 at 63.547 kb on - strand, within GFF4756 at 63.948 kb on + strand, within GFF4756 at 64.509 kb on - strand, within GFF4757 at 64.721 kb on + strand, within GFF4757
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 62,491 - GFF4754 0.15 -0.4 62,596 - GFF4754 0.28 -0.5 62,712 - GFF4754 0.43 +0.9 62,774 + GFF4754 0.51 -1.9 62,801 + GFF4754 0.54 -1.2 62,828 + GFF4754 0.57 +1.0 62,829 - GFF4754 0.58 +0.2 62,831 + GFF4754 0.58 -0.1 62,864 + GFF4754 0.62 +0.7 62,947 - GFF4754 0.72 -1.9 63,230 - GFF4755 0.24 -0.8 63,325 - GFF4755 0.60 +0.3 63,391 + GFF4755 0.85 -2.7 63,401 + GFF4755 0.89 -0.1 63,478 + +1.5 63,538 - GFF4756 0.12 -0.4 63,546 + GFF4756 0.14 -1.2 63,547 - GFF4756 0.14 -0.1 63,547 - GFF4756 0.14 -0.2 63,948 + GFF4756 0.73 +0.0 64,509 - GFF4757 0.43 -0.2 64,721 + GFF4757 0.66 +0.7
Or see this region's nucleotide sequence