Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4751 and GFF4752 are separated by 2 nucleotides GFF4752 and GFF4753 are separated by 137 nucleotides GFF4753 and GFF4754 overlap by 14 nucleotides GFF4754 and GFF4755 are separated by 3 nucleotides
GFF4751 - cell invasion protein SipB, at 58,897 to 60,678
GFF4751
GFF4752 - Type III secretion chaperone protein for YopD (SycD), at 60,681 to 61,178
GFF4752
GFF4753 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 61,316 to 62,386
GFF4753
GFF4754 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 62,373 to 63,164
GFF4754
GFF4755 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 63,168 to 63,428
GFF4755
Position (kb) 61
62
63 Strain fitness (log2 ratio) -2
-1
0
1 at 60.395 kb on - strand, within GFF4751 at 60.403 kb on + strand, within GFF4751 at 60.403 kb on + strand, within GFF4751 at 60.403 kb on + strand, within GFF4751 at 60.404 kb on - strand, within GFF4751 at 60.640 kb on - strand at 60.821 kb on + strand, within GFF4752 at 60.939 kb on + strand, within GFF4752 at 60.940 kb on - strand, within GFF4752 at 61.060 kb on - strand, within GFF4752 at 61.074 kb on - strand, within GFF4752 at 61.164 kb on - strand at 61.184 kb on - strand at 61.210 kb on + strand at 61.301 kb on - strand at 61.309 kb on - strand at 61.510 kb on - strand, within GFF4753 at 61.802 kb on + strand, within GFF4753 at 61.806 kb on - strand, within GFF4753 at 61.919 kb on - strand, within GFF4753 at 62.132 kb on - strand, within GFF4753 at 62.212 kb on + strand, within GFF4753 at 62.253 kb on + strand, within GFF4753 at 62.491 kb on - strand, within GFF4754 at 62.596 kb on - strand, within GFF4754 at 62.712 kb on - strand, within GFF4754 at 62.774 kb on + strand, within GFF4754 at 62.801 kb on + strand, within GFF4754 at 62.828 kb on + strand, within GFF4754 at 62.829 kb on - strand, within GFF4754 at 62.831 kb on + strand, within GFF4754 at 62.864 kb on + strand, within GFF4754 at 62.947 kb on - strand, within GFF4754 at 63.230 kb on - strand, within GFF4755 at 63.325 kb on - strand, within GFF4755
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 60,395 - GFF4751 0.84 +1.5 60,403 + GFF4751 0.85 +0.0 60,403 + GFF4751 0.85 +0.5 60,403 + GFF4751 0.85 -0.1 60,404 - GFF4751 0.85 -0.5 60,640 - -1.4 60,821 + GFF4752 0.28 -0.1 60,939 + GFF4752 0.52 +0.5 60,940 - GFF4752 0.52 -1.0 61,060 - GFF4752 0.76 -1.2 61,074 - GFF4752 0.79 -1.1 61,164 - -2.3 61,184 - +0.1 61,210 + +0.5 61,301 - -0.7 61,309 - -0.5 61,510 - GFF4753 0.18 -0.1 61,802 + GFF4753 0.45 -1.1 61,806 - GFF4753 0.46 -0.8 61,919 - GFF4753 0.56 -0.7 62,132 - GFF4753 0.76 -1.2 62,212 + GFF4753 0.84 +0.5 62,253 + GFF4753 0.87 -0.3 62,491 - GFF4754 0.15 -0.4 62,596 - GFF4754 0.28 -0.5 62,712 - GFF4754 0.43 +0.9 62,774 + GFF4754 0.51 -1.9 62,801 + GFF4754 0.54 -1.2 62,828 + GFF4754 0.57 +1.0 62,829 - GFF4754 0.58 +0.2 62,831 + GFF4754 0.58 -0.1 62,864 + GFF4754 0.62 +0.7 62,947 - GFF4754 0.72 -1.9 63,230 - GFF4755 0.24 -0.8 63,325 - GFF4755 0.60 +0.3
Or see this region's nucleotide sequence