Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4597 and GFF4598 are separated by 51 nucleotides GFF4598 and GFF4599 are separated by 133 nucleotides GFF4599 and GFF4600 are separated by 184 nucleotides GFF4600 and GFF4601 are separated by 24 nucleotides
GFF4597 - Flavodoxin 1, at 387,672 to 388,301
GFF4597
GFF4598 - Uncharacterized protein ybfE, at 388,353 to 388,646
GFF4598
GFF4599 - Esterase ybfF (EC 3.1.-.-), at 388,780 to 389,550
GFF4599
GFF4600 - SeqA protein, negative modulator of initiation of replication, at 389,735 to 390,277
GFF4600
GFF4601 - Phosphoglucomutase (EC 5.4.2.2), at 390,302 to 391,942
GFF4601
Position (kb) 388
389
390 Strain fitness (log2 ratio) -2
-1
0
1
2 at 388.382 kb on + strand at 388.395 kb on + strand, within GFF4598 at 388.545 kb on + strand, within GFF4598 at 388.545 kb on + strand, within GFF4598 at 388.614 kb on + strand, within GFF4598 at 388.623 kb on - strand at 388.711 kb on + strand at 388.712 kb on - strand at 388.712 kb on - strand at 388.844 kb on - strand at 388.901 kb on - strand, within GFF4599 at 388.929 kb on - strand, within GFF4599 at 388.998 kb on + strand, within GFF4599 at 389.017 kb on + strand, within GFF4599 at 389.018 kb on - strand, within GFF4599 at 389.021 kb on - strand, within GFF4599 at 389.106 kb on + strand, within GFF4599 at 389.195 kb on + strand, within GFF4599 at 389.386 kb on + strand, within GFF4599 at 389.505 kb on + strand at 389.589 kb on + strand at 389.590 kb on - strand at 389.623 kb on + strand at 389.630 kb on + strand at 389.687 kb on + strand at 389.771 kb on + strand at 389.772 kb on - strand at 389.827 kb on + strand, within GFF4600 at 390.142 kb on + strand, within GFF4600 at 390.143 kb on - strand, within GFF4600 at 390.143 kb on - strand, within GFF4600 at 390.143 kb on - strand, within GFF4600 at 390.276 kb on - strand at 390.276 kb on - strand at 390.287 kb on + strand at 390.287 kb on + strand at 390.380 kb on + strand at 390.380 kb on + strand at 390.381 kb on - strand at 390.384 kb on + strand at 390.545 kb on + strand, within GFF4601 at 390.546 kb on - strand, within GFF4601 at 390.546 kb on - strand, within GFF4601 at 390.546 kb on - strand, within GFF4601
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 388,382 + +0.0 388,395 + GFF4598 0.14 -1.6 388,545 + GFF4598 0.65 -2.1 388,545 + GFF4598 0.65 -0.2 388,614 + GFF4598 0.89 -0.7 388,623 - +0.1 388,711 + +0.6 388,712 - -1.9 388,712 - +0.3 388,844 - +0.0 388,901 - GFF4599 0.16 -0.7 388,929 - GFF4599 0.19 -1.4 388,998 + GFF4599 0.28 +0.5 389,017 + GFF4599 0.31 +0.1 389,018 - GFF4599 0.31 -0.3 389,021 - GFF4599 0.31 +0.1 389,106 + GFF4599 0.42 -1.9 389,195 + GFF4599 0.54 -0.3 389,386 + GFF4599 0.79 +0.5 389,505 + +0.4 389,589 + -0.4 389,590 - +0.3 389,623 + -1.5 389,630 + -0.2 389,687 + -0.4 389,771 + +0.3 389,772 - +1.8 389,827 + GFF4600 0.17 +0.8 390,142 + GFF4600 0.75 +0.9 390,143 - GFF4600 0.75 +0.3 390,143 - GFF4600 0.75 +1.9 390,143 - GFF4600 0.75 +1.7 390,276 - -1.4 390,276 - +1.6 390,287 + -0.8 390,287 + -0.3 390,380 + +1.1 390,380 + +1.7 390,381 - -0.5 390,384 + +0.2 390,545 + GFF4601 0.15 -0.7 390,546 - GFF4601 0.15 -0.3 390,546 - GFF4601 0.15 +1.7 390,546 - GFF4601 0.15 -1.9
Or see this region's nucleotide sequence