Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4594 and GFF4595 are separated by 97 nucleotides GFF4595 and GFF4596 are separated by 114 nucleotides GFF4596 and GFF4597 are separated by 281 nucleotides
GFF4594 - TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate, at 384,396 to 385,799
GFF4594
GFF4595 - TcuR: regulates tcuABC genes used in utilization of tricarballylate, at 385,897 to 386,823
GFF4595
GFF4596 - Ferric uptake regulation protein FUR, at 386,938 to 387,390
GFF4596
GFF4597 - Flavodoxin 1, at 387,672 to 388,301
GFF4597
Position (kb) 385
386
387 Strain fitness (log2 ratio) -2
-1
0
1
2 at 385.158 kb on - strand, within GFF4594 at 385.269 kb on + strand, within GFF4594 at 385.400 kb on - strand, within GFF4594 at 385.621 kb on - strand, within GFF4594 at 385.621 kb on - strand, within GFF4594 at 385.649 kb on + strand, within GFF4594 at 385.650 kb on - strand, within GFF4594 at 385.759 kb on - strand at 385.789 kb on - strand at 385.802 kb on + strand at 385.803 kb on - strand at 385.804 kb on + strand at 385.805 kb on - strand at 386.480 kb on + strand, within GFF4595 at 386.511 kb on + strand, within GFF4595 at 386.772 kb on + strand at 386.791 kb on - strand at 386.807 kb on - strand at 386.940 kb on - strand at 387.009 kb on - strand, within GFF4596 at 387.225 kb on - strand, within GFF4596 at 387.272 kb on + strand, within GFF4596 at 387.368 kb on - strand at 387.470 kb on - strand at 387.561 kb on + strand at 387.577 kb on - strand at 387.608 kb on - strand at 387.650 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 385,158 - GFF4594 0.54 +0.0 385,269 + GFF4594 0.62 +0.2 385,400 - GFF4594 0.72 -0.4 385,621 - GFF4594 0.87 -1.0 385,621 - GFF4594 0.87 +0.3 385,649 + GFF4594 0.89 +0.4 385,650 - GFF4594 0.89 -0.0 385,759 - +0.1 385,789 - -0.2 385,802 + +0.2 385,803 - -0.6 385,804 + -0.1 385,805 - +0.5 386,480 + GFF4595 0.63 -2.4 386,511 + GFF4595 0.66 +0.1 386,772 + +0.3 386,791 - -1.1 386,807 - -0.2 386,940 - +1.2 387,009 - GFF4596 0.16 +2.1 387,225 - GFF4596 0.63 -0.1 387,272 + GFF4596 0.74 -0.3 387,368 - +0.5 387,470 - -1.2 387,561 + +0.7 387,577 - +0.3 387,608 - -0.1 387,650 + +0.3
Or see this region's nucleotide sequence