Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4590 and GFF4591 are separated by 49 nucleotides GFF4591 and GFF4592 are separated by 48 nucleotides GFF4592 and GFF4593 are separated by 50 nucleotides
GFF4590 - N-acetylglucosamine-regulated outer membrane porin, at 380,078 to 381,484
GFF4590
GFF4591 - Hypothetical lipoprotein ybfN precursor, at 381,534 to 381,866
GFF4591
GFF4592 - TcuC: integral membrane protein used to transport tricarballylate across the cell membrane, at 381,915 to 383,219
GFF4592
GFF4593 - TcuB: works with TcuA to oxidize tricarballylate to cis-aconitate, at 383,270 to 384,409
GFF4593
Position (kb) 381
382
383
384 Strain fitness (log2 ratio) -2
-1
0
1
2 at 380.932 kb on + strand, within GFF4590 at 380.937 kb on - strand, within GFF4590 at 380.937 kb on - strand, within GFF4590 at 381.045 kb on - strand, within GFF4590 at 381.058 kb on + strand, within GFF4590 at 381.071 kb on + strand, within GFF4590 at 381.164 kb on + strand, within GFF4590 at 381.165 kb on - strand, within GFF4590 at 381.213 kb on - strand, within GFF4590 at 381.266 kb on + strand, within GFF4590 at 381.266 kb on + strand, within GFF4590 at 381.302 kb on + strand, within GFF4590 at 381.321 kb on - strand, within GFF4590 at 381.395 kb on - strand at 381.534 kb on - strand at 381.631 kb on - strand, within GFF4591 at 381.674 kb on - strand, within GFF4591 at 381.762 kb on + strand, within GFF4591 at 381.762 kb on + strand, within GFF4591 at 381.854 kb on + strand at 381.940 kb on + strand at 381.991 kb on - strand at 381.991 kb on - strand at 382.133 kb on - strand, within GFF4592 at 382.133 kb on - strand, within GFF4592 at 382.133 kb on - strand, within GFF4592 at 382.133 kb on - strand, within GFF4592 at 382.177 kb on + strand, within GFF4592 at 382.178 kb on - strand, within GFF4592 at 382.289 kb on + strand, within GFF4592 at 382.290 kb on - strand, within GFF4592 at 382.456 kb on + strand, within GFF4592 at 382.456 kb on + strand, within GFF4592 at 382.503 kb on - strand, within GFF4592 at 382.689 kb on - strand, within GFF4592 at 382.697 kb on + strand, within GFF4592 at 382.799 kb on - strand, within GFF4592 at 382.984 kb on + strand, within GFF4592 at 383.101 kb on + strand at 383.101 kb on + strand at 383.102 kb on - strand at 383.141 kb on - strand at 383.257 kb on - strand at 383.662 kb on - strand, within GFF4593 at 383.705 kb on - strand, within GFF4593 at 383.886 kb on - strand, within GFF4593 at 383.950 kb on + strand, within GFF4593 at 384.084 kb on + strand, within GFF4593
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 380,932 + GFF4590 0.61 +1.3 380,937 - GFF4590 0.61 +0.9 380,937 - GFF4590 0.61 -1.8 381,045 - GFF4590 0.69 -0.6 381,058 + GFF4590 0.70 +0.3 381,071 + GFF4590 0.71 -0.5 381,164 + GFF4590 0.77 +0.1 381,165 - GFF4590 0.77 +0.0 381,213 - GFF4590 0.81 -0.0 381,266 + GFF4590 0.84 -1.6 381,266 + GFF4590 0.84 -0.2 381,302 + GFF4590 0.87 -0.1 381,321 - GFF4590 0.88 -1.4 381,395 - -1.8 381,534 - -0.3 381,631 - GFF4591 0.29 +0.6 381,674 - GFF4591 0.42 +0.1 381,762 + GFF4591 0.68 +0.6 381,762 + GFF4591 0.68 -0.2 381,854 + -1.6 381,940 + +0.1 381,991 - -1.7 381,991 - -0.2 382,133 - GFF4592 0.17 +0.7 382,133 - GFF4592 0.17 +0.1 382,133 - GFF4592 0.17 -0.5 382,133 - GFF4592 0.17 -0.4 382,177 + GFF4592 0.20 -0.6 382,178 - GFF4592 0.20 +0.1 382,289 + GFF4592 0.29 -0.2 382,290 - GFF4592 0.29 +0.4 382,456 + GFF4592 0.41 +0.0 382,456 + GFF4592 0.41 -0.3 382,503 - GFF4592 0.45 -1.2 382,689 - GFF4592 0.59 +2.3 382,697 + GFF4592 0.60 +0.1 382,799 - GFF4592 0.68 -0.8 382,984 + GFF4592 0.82 +0.3 383,101 + +0.9 383,101 + -0.7 383,102 - -1.6 383,141 - -0.3 383,257 - -1.8 383,662 - GFF4593 0.34 -0.1 383,705 - GFF4593 0.38 -0.3 383,886 - GFF4593 0.54 -0.2 383,950 + GFF4593 0.60 +0.3 384,084 + GFF4593 0.71 +0.6
Or see this region's nucleotide sequence