Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4571 and GFF4572 overlap by 1 nucleotides GFF4572 and GFF4573 are separated by 159 nucleotides GFF4573 and GFF4574 are separated by 354 nucleotides
GFF4571 - Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4), at 357,549 to 358,223
GFF4571
GFF4572 - Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4), at 358,223 to 358,963
GFF4572
GFF4573 - Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4), at 359,123 to 360,031
GFF4573
GFF4574 - Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE, at 360,386 to 361,975
GFF4574
Position (kb)
359
360
361 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 358.180 kb on + strand at 358.288 kb on + strand at 358.308 kb on + strand, within GFF4572 at 358.308 kb on + strand, within GFF4572 at 358.309 kb on - strand, within GFF4572 at 358.498 kb on - strand, within GFF4572 at 358.744 kb on + strand, within GFF4572 at 358.744 kb on + strand, within GFF4572 at 358.757 kb on + strand, within GFF4572 at 358.761 kb on + strand, within GFF4572 at 358.806 kb on - strand, within GFF4572 at 358.819 kb on + strand, within GFF4572 at 358.903 kb on - strand at 358.955 kb on + strand at 358.963 kb on + strand at 358.963 kb on + strand at 358.963 kb on + strand at 358.964 kb on - strand at 358.964 kb on - strand at 359.061 kb on + strand at 359.061 kb on + strand at 359.061 kb on + strand at 359.063 kb on + strand at 359.063 kb on + strand at 359.064 kb on - strand at 359.064 kb on - strand at 359.137 kb on + strand at 359.231 kb on - strand, within GFF4573 at 359.323 kb on + strand, within GFF4573 at 359.324 kb on - strand, within GFF4573 at 359.425 kb on + strand, within GFF4573 at 359.489 kb on - strand, within GFF4573 at 359.492 kb on - strand, within GFF4573 at 359.524 kb on + strand, within GFF4573 at 359.525 kb on - strand, within GFF4573 at 359.789 kb on - strand, within GFF4573 at 359.865 kb on + strand, within GFF4573 at 360.023 kb on + strand at 360.023 kb on + strand at 360.024 kb on - strand at 360.032 kb on - strand at 360.044 kb on + strand at 360.070 kb on + strand at 360.071 kb on - strand at 360.071 kb on - strand at 360.312 kb on - strand at 360.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 358,180 + +1.1 358,288 + -0.0 358,308 + GFF4572 0.11 -0.5 358,308 + GFF4572 0.11 +0.6 358,309 - GFF4572 0.12 -1.9 358,498 - GFF4572 0.37 -0.2 358,744 + GFF4572 0.70 +0.0 358,744 + GFF4572 0.70 -0.7 358,757 + GFF4572 0.72 +0.3 358,761 + GFF4572 0.73 +1.2 358,806 - GFF4572 0.79 -0.1 358,819 + GFF4572 0.80 -0.8 358,903 - -1.3 358,955 + +0.2 358,963 + +0.7 358,963 + -0.6 358,963 + -0.5 358,964 - -0.6 358,964 - -0.2 359,061 + -3.0 359,061 + +1.7 359,061 + -0.0 359,063 + +1.7 359,063 + -0.4 359,064 - -1.9 359,064 - +0.5 359,137 + -0.9 359,231 - GFF4573 0.12 -1.4 359,323 + GFF4573 0.22 -0.1 359,324 - GFF4573 0.22 -0.0 359,425 + GFF4573 0.33 +1.2 359,489 - GFF4573 0.40 -1.7 359,492 - GFF4573 0.41 +1.3 359,524 + GFF4573 0.44 -0.9 359,525 - GFF4573 0.44 -1.8 359,789 - GFF4573 0.73 -0.6 359,865 + GFF4573 0.82 -0.1 360,023 + -0.2 360,023 + +0.7 360,024 - -0.6 360,032 - -0.7 360,044 + +1.3 360,070 + +0.0 360,071 - +0.3 360,071 - +1.2 360,312 - -1.9 360,388 - +0.2
Or see this region's nucleotide sequence