Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4524 and GFF4525 are separated by 37 nucleotides GFF4525 and GFF4526 overlap by 26 nucleotides GFF4526 and GFF4527 are separated by 3 nucleotides
GFF4524 - Citrate Succinate antiporter (TC 2.A.47.3.2), at 311,119 to 312,582
GFF4524
GFF4525 - '2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme- A synthase (EC 2.7.8.25)' transl_table=11, at 312,620 to 313,513
GFF4525
GFF4526 - Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.61), at 313,488 to 314,039
GFF4526
GFF4527 - Citrate lyase alpha chain (EC 4.1.3.6), at 314,043 to 315,572
GFF4527
Position (kb) 313
314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 312.495 kb on + strand at 312.495 kb on + strand at 312.770 kb on + strand, within GFF4525 at 312.773 kb on + strand, within GFF4525 at 312.871 kb on + strand, within GFF4525 at 313.002 kb on + strand, within GFF4525 at 313.028 kb on + strand, within GFF4525 at 313.029 kb on - strand, within GFF4525 at 313.029 kb on - strand, within GFF4525 at 313.118 kb on + strand, within GFF4525 at 313.118 kb on + strand, within GFF4525 at 313.118 kb on + strand, within GFF4525 at 313.119 kb on - strand, within GFF4525 at 313.644 kb on + strand, within GFF4526 at 313.816 kb on + strand, within GFF4526 at 313.911 kb on - strand, within GFF4526 at 314.044 kb on + strand at 314.507 kb on + strand, within GFF4527 at 314.776 kb on + strand, within GFF4527
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 312,495 + +0.1 312,495 + -0.1 312,770 + GFF4525 0.17 -1.3 312,773 + GFF4525 0.17 -0.3 312,871 + GFF4525 0.28 +0.8 313,002 + GFF4525 0.43 +0.6 313,028 + GFF4525 0.46 +2.5 313,029 - GFF4525 0.46 -0.5 313,029 - GFF4525 0.46 -0.6 313,118 + GFF4525 0.56 +0.2 313,118 + GFF4525 0.56 -0.3 313,118 + GFF4525 0.56 -0.7 313,119 - GFF4525 0.56 +1.0 313,644 + GFF4526 0.28 -0.2 313,816 + GFF4526 0.59 -3.1 313,911 - GFF4526 0.77 -0.3 314,044 + -1.2 314,507 + GFF4527 0.30 +0.1 314,776 + GFF4527 0.48 +0.3
Or see this region's nucleotide sequence