Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4491 and GFF4492 are separated by 30 nucleotides GFF4492 and GFF4493 overlap by 4 nucleotides GFF4493 and GFF4494 are separated by 249 nucleotides
GFF4491 - Enterobactin esterase, at 274,216 to 275,430
GFF4491
GFF4492 - FIG005032: Putative cytoplasmic protein YbdZ in enterobactin biosynthesis operon, at 275,461 to 275,679
GFF4492
GFF4493 - Enterobactin synthetase component F, serine activating enzyme (EC 2.7.7.-), at 275,676 to 279,560
GFF4493
GFF4494 - Ferric enterobactin uptake protein FepE, at 279,810 to 280,946
GFF4494
Position (kb) 275
276
277
278
279
280 Strain fitness (log2 ratio) -2
-1
0
1
2 at 274.773 kb on - strand, within GFF4491 at 274.822 kb on - strand, within GFF4491 at 274.938 kb on + strand, within GFF4491 at 275.133 kb on - strand, within GFF4491 at 275.310 kb on + strand at 275.619 kb on + strand, within GFF4492 at 275.769 kb on + strand at 275.769 kb on + strand at 275.769 kb on + strand at 275.770 kb on - strand at 276.040 kb on + strand at 276.217 kb on - strand, within GFF4493 at 276.412 kb on + strand, within GFF4493 at 276.412 kb on + strand, within GFF4493 at 276.549 kb on - strand, within GFF4493 at 276.625 kb on + strand, within GFF4493 at 276.763 kb on + strand, within GFF4493 at 277.011 kb on - strand, within GFF4493 at 277.011 kb on - strand, within GFF4493 at 277.016 kb on + strand, within GFF4493 at 277.343 kb on - strand, within GFF4493 at 277.406 kb on + strand, within GFF4493 at 277.406 kb on + strand, within GFF4493 at 277.464 kb on - strand, within GFF4493 at 277.471 kb on - strand, within GFF4493 at 277.471 kb on - strand, within GFF4493 at 277.589 kb on - strand, within GFF4493 at 277.592 kb on - strand, within GFF4493 at 277.728 kb on + strand, within GFF4493 at 277.729 kb on - strand, within GFF4493 at 277.823 kb on - strand, within GFF4493 at 277.912 kb on - strand, within GFF4493 at 278.030 kb on + strand, within GFF4493 at 278.031 kb on - strand, within GFF4493 at 278.045 kb on + strand, within GFF4493 at 278.082 kb on + strand, within GFF4493 at 278.181 kb on + strand, within GFF4493 at 278.273 kb on + strand, within GFF4493 at 278.273 kb on + strand, within GFF4493 at 278.881 kb on + strand, within GFF4493 at 278.882 kb on - strand, within GFF4493 at 278.926 kb on + strand, within GFF4493 at 279.022 kb on + strand, within GFF4493 at 279.076 kb on - strand, within GFF4493 at 279.076 kb on - strand, within GFF4493 at 279.079 kb on + strand, within GFF4493 at 279.082 kb on + strand, within GFF4493 at 279.236 kb on - strand at 279.386 kb on + strand at 279.386 kb on + strand at 279.387 kb on - strand at 279.494 kb on - strand at 279.658 kb on + strand at 279.906 kb on - strand at 280.281 kb on - strand, within GFF4494 at 280.386 kb on + strand, within GFF4494 at 280.387 kb on - strand, within GFF4494 at 280.387 kb on - strand, within GFF4494
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 274,773 - GFF4491 0.46 -1.4 274,822 - GFF4491 0.50 +1.0 274,938 + GFF4491 0.59 +0.6 275,133 - GFF4491 0.75 -2.1 275,310 + -1.6 275,619 + GFF4492 0.72 +0.1 275,769 + +1.1 275,769 + -0.0 275,769 + -0.5 275,770 - -1.1 276,040 + +0.3 276,217 - GFF4493 0.14 -1.5 276,412 + GFF4493 0.19 -0.4 276,412 + GFF4493 0.19 -0.2 276,549 - GFF4493 0.22 +0.6 276,625 + GFF4493 0.24 +0.4 276,763 + GFF4493 0.28 +1.1 277,011 - GFF4493 0.34 -1.0 277,011 - GFF4493 0.34 -0.8 277,016 + GFF4493 0.34 -0.2 277,343 - GFF4493 0.43 +1.0 277,406 + GFF4493 0.45 +0.7 277,406 + GFF4493 0.45 -0.5 277,464 - GFF4493 0.46 +0.3 277,471 - GFF4493 0.46 -1.3 277,471 - GFF4493 0.46 -0.2 277,589 - GFF4493 0.49 -0.5 277,592 - GFF4493 0.49 -0.6 277,728 + GFF4493 0.53 -2.5 277,729 - GFF4493 0.53 +0.9 277,823 - GFF4493 0.55 -2.1 277,912 - GFF4493 0.58 +2.6 278,030 + GFF4493 0.61 -0.5 278,031 - GFF4493 0.61 -0.0 278,045 + GFF4493 0.61 -0.6 278,082 + GFF4493 0.62 -0.0 278,181 + GFF4493 0.64 +0.3 278,273 + GFF4493 0.67 -2.2 278,273 + GFF4493 0.67 -0.8 278,881 + GFF4493 0.82 -1.5 278,882 - GFF4493 0.83 +0.2 278,926 + GFF4493 0.84 -0.7 279,022 + GFF4493 0.86 -1.0 279,076 - GFF4493 0.88 -0.2 279,076 - GFF4493 0.88 -2.6 279,079 + GFF4493 0.88 +2.7 279,082 + GFF4493 0.88 -1.0 279,236 - -0.1 279,386 + -0.4 279,386 + +0.3 279,387 - -2.0 279,494 - +1.2 279,658 + +0.1 279,906 - +0.3 280,281 - GFF4494 0.41 +1.0 280,386 + GFF4494 0.51 +0.0 280,387 - GFF4494 0.51 +0.0 280,387 - GFF4494 0.51 -0.3
Or see this region's nucleotide sequence