Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4308 and GFF4309 are separated by 147 nucleotides GFF4309 and GFF4310 are separated by 25 nucleotides GFF4310 and GFF4311 are separated by 169 nucleotides
GFF4308 - Protein-export membrane protein SecF (TC 3.A.5.1.1), at 94,401 to 95,372
GFF4308
GFF4309 - Glyoxalase family protein, at 95,520 to 95,885
GFF4309
GFF4310 - Transcriptional regulator, DeoR family, at 95,911 to 96,603
GFF4310
GFF4311 - hypothetical protein, at 96,773 to 97,120
GFF4311
Position (kb)
95
96 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 95.300 kb on + strand at 95.315 kb on + strand at 95.315 kb on + strand at 95.315 kb on + strand at 95.379 kb on - strand at 95.382 kb on + strand at 95.382 kb on + strand at 95.390 kb on - strand at 95.427 kb on + strand at 95.431 kb on + strand at 95.450 kb on - strand at 95.458 kb on + strand at 95.480 kb on + strand at 95.481 kb on - strand at 95.602 kb on + strand, within GFF4309 at 95.602 kb on + strand, within GFF4309 at 95.653 kb on - strand, within GFF4309 at 95.723 kb on + strand, within GFF4309 at 95.841 kb on - strand, within GFF4309 at 95.864 kb on - strand at 95.869 kb on + strand at 95.869 kb on + strand at 95.870 kb on - strand at 95.887 kb on - strand at 95.893 kb on + strand at 96.127 kb on - strand, within GFF4310 at 96.127 kb on - strand, within GFF4310 at 96.159 kb on + strand, within GFF4310 at 96.168 kb on - strand, within GFF4310 at 96.280 kb on + strand, within GFF4310 at 96.331 kb on - strand, within GFF4310 at 96.410 kb on + strand, within GFF4310 at 96.411 kb on - strand, within GFF4310 at 96.411 kb on - strand, within GFF4310 at 96.411 kb on - strand, within GFF4310 at 96.677 kb on + strand at 96.687 kb on + strand at 96.687 kb on + strand at 96.689 kb on + strand at 96.689 kb on + strand at 96.689 kb on + strand at 96.690 kb on - strand at 96.690 kb on - strand at 96.769 kb on - strand at 96.772 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 95,300 + -0.5 95,315 + -0.4 95,315 + -2.2 95,315 + +0.8 95,379 - -1.2 95,382 + -0.5 95,382 + +0.3 95,390 - +0.9 95,427 + -0.6 95,431 + +0.6 95,450 - -0.8 95,458 + -0.3 95,480 + -1.6 95,481 - -0.1 95,602 + GFF4309 0.22 -0.4 95,602 + GFF4309 0.22 -0.6 95,653 - GFF4309 0.36 -1.0 95,723 + GFF4309 0.55 -1.2 95,841 - GFF4309 0.88 -1.6 95,864 - +0.1 95,869 + -0.0 95,869 + +0.7 95,870 - -0.3 95,887 - -1.6 95,893 + +0.7 96,127 - GFF4310 0.31 +0.7 96,127 - GFF4310 0.31 -0.4 96,159 + GFF4310 0.36 -0.9 96,168 - GFF4310 0.37 +2.3 96,280 + GFF4310 0.53 -0.1 96,331 - GFF4310 0.61 -0.4 96,410 + GFF4310 0.72 -1.0 96,411 - GFF4310 0.72 -0.9 96,411 - GFF4310 0.72 -0.3 96,411 - GFF4310 0.72 +0.3 96,677 + -0.9 96,687 + -0.1 96,687 + -0.7 96,689 + -1.3 96,689 + +0.1 96,689 + -1.4 96,690 - +0.6 96,690 - +0.2 96,769 - +0.3 96,772 + -0.1
Or see this region's nucleotide sequence