Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4267 and GFF4268 are separated by 219 nucleotides GFF4268 and GFF4269 are separated by 314 nucleotides GFF4269 and GFF4270 are separated by 85 nucleotides GFF4270 and GFF4271 are separated by 44 nucleotides
GFF4267 - Porphobilinogen synthase (EC 4.2.1.24), at 53,890 to 54,864
GFF4267
GFF4268 - hypothetical protein, at 55,084 to 55,203
GFF4268
GFF4269 - Putative flagellin structural protein, at 55,518 to 58,313
GFF4269
GFF4270 - FIG00637869: hypothetical protein, at 58,399 to 59,022
GFF4270
GFF4271 - Penicillin-binding protein AmpH, at 59,067 to 60,224
GFF4271
Position (kb)
55
56
57
58
59 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 55.460 kb on + strand at 55.479 kb on + strand at 55.833 kb on + strand, within GFF4269 at 56.143 kb on - strand, within GFF4269 at 56.160 kb on + strand, within GFF4269 at 56.165 kb on + strand, within GFF4269 at 56.165 kb on + strand, within GFF4269 at 56.280 kb on + strand, within GFF4269 at 56.381 kb on + strand, within GFF4269 at 56.381 kb on + strand, within GFF4269 at 56.456 kb on + strand, within GFF4269 at 56.457 kb on - strand, within GFF4269 at 56.496 kb on + strand, within GFF4269 at 56.496 kb on + strand, within GFF4269 at 56.523 kb on + strand, within GFF4269 at 56.523 kb on + strand, within GFF4269 at 56.524 kb on - strand, within GFF4269 at 56.570 kb on + strand, within GFF4269 at 56.571 kb on - strand, within GFF4269 at 56.724 kb on + strand, within GFF4269 at 56.821 kb on - strand, within GFF4269 at 56.822 kb on + strand, within GFF4269 at 56.823 kb on - strand, within GFF4269 at 56.825 kb on + strand, within GFF4269 at 56.879 kb on + strand, within GFF4269 at 56.957 kb on + strand, within GFF4269 at 57.000 kb on - strand, within GFF4269 at 57.023 kb on + strand, within GFF4269 at 57.023 kb on + strand, within GFF4269 at 57.039 kb on + strand, within GFF4269 at 57.040 kb on - strand, within GFF4269 at 57.072 kb on - strand, within GFF4269 at 57.080 kb on + strand, within GFF4269 at 57.081 kb on - strand, within GFF4269 at 57.096 kb on + strand, within GFF4269 at 57.222 kb on + strand, within GFF4269 at 57.304 kb on + strand, within GFF4269 at 57.305 kb on - strand, within GFF4269 at 57.354 kb on + strand, within GFF4269 at 57.354 kb on + strand, within GFF4269 at 57.355 kb on - strand, within GFF4269 at 57.360 kb on + strand, within GFF4269 at 57.360 kb on + strand, within GFF4269 at 57.417 kb on + strand, within GFF4269 at 57.422 kb on + strand, within GFF4269 at 57.488 kb on + strand, within GFF4269 at 57.547 kb on - strand, within GFF4269 at 57.585 kb on - strand, within GFF4269 at 57.585 kb on - strand, within GFF4269 at 57.602 kb on + strand, within GFF4269 at 57.650 kb on + strand, within GFF4269 at 57.651 kb on - strand, within GFF4269 at 57.744 kb on + strand, within GFF4269 at 57.744 kb on + strand, within GFF4269 at 57.744 kb on + strand, within GFF4269 at 57.839 kb on + strand, within GFF4269 at 57.843 kb on + strand, within GFF4269 at 57.843 kb on + strand, within GFF4269 at 57.844 kb on - strand, within GFF4269 at 57.844 kb on - strand, within GFF4269 at 57.844 kb on - strand, within GFF4269 at 57.928 kb on + strand, within GFF4269 at 57.928 kb on + strand, within GFF4269 at 57.928 kb on + strand, within GFF4269 at 57.929 kb on - strand, within GFF4269 at 58.055 kb on + strand at 58.055 kb on + strand at 58.065 kb on + strand at 58.066 kb on - strand at 58.066 kb on - strand at 58.141 kb on + strand at 58.168 kb on + strand at 58.249 kb on - strand at 58.299 kb on - strand at 58.304 kb on + strand at 58.398 kb on + strand at 58.399 kb on - strand at 58.519 kb on - strand, within GFF4270 at 59.048 kb on + strand at 59.139 kb on - strand at 59.192 kb on + strand, within GFF4271 at 59.215 kb on + strand, within GFF4271 at 59.283 kb on - strand, within GFF4271 at 59.284 kb on + strand, within GFF4271 at 59.284 kb on + strand, within GFF4271
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 55,460 + +1.7 55,479 + -1.4 55,833 + GFF4269 0.11 +1.3 56,143 - GFF4269 0.22 -1.6 56,160 + GFF4269 0.23 +1.4 56,165 + GFF4269 0.23 +0.7 56,165 + GFF4269 0.23 +1.8 56,280 + GFF4269 0.27 +0.7 56,381 + GFF4269 0.31 +0.5 56,381 + GFF4269 0.31 -0.3 56,456 + GFF4269 0.34 -1.4 56,457 - GFF4269 0.34 +1.4 56,496 + GFF4269 0.35 +0.9 56,496 + GFF4269 0.35 -0.0 56,523 + GFF4269 0.36 -0.5 56,523 + GFF4269 0.36 -0.4 56,524 - GFF4269 0.36 +0.3 56,570 + GFF4269 0.38 +0.7 56,571 - GFF4269 0.38 -1.4 56,724 + GFF4269 0.43 -0.5 56,821 - GFF4269 0.47 -0.1 56,822 + GFF4269 0.47 -0.2 56,823 - GFF4269 0.47 -1.1 56,825 + GFF4269 0.47 -0.1 56,879 + GFF4269 0.49 -0.0 56,957 + GFF4269 0.51 +0.1 57,000 - GFF4269 0.53 +0.1 57,023 + GFF4269 0.54 -0.2 57,023 + GFF4269 0.54 -0.3 57,039 + GFF4269 0.54 -0.1 57,040 - GFF4269 0.54 +0.1 57,072 - GFF4269 0.56 -1.3 57,080 + GFF4269 0.56 +0.7 57,081 - GFF4269 0.56 -0.9 57,096 + GFF4269 0.56 -0.3 57,222 + GFF4269 0.61 -0.6 57,304 + GFF4269 0.64 -0.6 57,305 - GFF4269 0.64 +2.4 57,354 + GFF4269 0.66 -0.6 57,354 + GFF4269 0.66 -0.1 57,355 - GFF4269 0.66 +1.2 57,360 + GFF4269 0.66 -0.5 57,360 + GFF4269 0.66 +0.5 57,417 + GFF4269 0.68 -0.0 57,422 + GFF4269 0.68 -1.0 57,488 + GFF4269 0.70 -2.8 57,547 - GFF4269 0.73 +0.0 57,585 - GFF4269 0.74 -0.0 57,585 - GFF4269 0.74 -0.4 57,602 + GFF4269 0.75 -0.1 57,650 + GFF4269 0.76 -0.1 57,651 - GFF4269 0.76 -0.4 57,744 + GFF4269 0.80 -0.7 57,744 + GFF4269 0.80 -0.8 57,744 + GFF4269 0.80 -0.7 57,839 + GFF4269 0.83 -0.6 57,843 + GFF4269 0.83 -0.3 57,843 + GFF4269 0.83 +1.7 57,844 - GFF4269 0.83 +0.8 57,844 - GFF4269 0.83 +0.0 57,844 - GFF4269 0.83 -0.6 57,928 + GFF4269 0.86 +0.2 57,928 + GFF4269 0.86 -1.3 57,928 + GFF4269 0.86 -0.6 57,929 - GFF4269 0.86 +3.3 58,055 + -0.9 58,055 + +0.2 58,065 + -1.7 58,066 - -1.1 58,066 - -1.5 58,141 + +0.0 58,168 + +0.0 58,249 - -0.8 58,299 - -0.1 58,304 + -0.4 58,398 + +1.0 58,399 - -0.4 58,519 - GFF4270 0.19 +1.0 59,048 + -0.6 59,139 - -1.2 59,192 + GFF4271 0.11 +0.3 59,215 + GFF4271 0.13 +0.9 59,283 - GFF4271 0.19 +1.4 59,284 + GFF4271 0.19 +0.1 59,284 + GFF4271 0.19 +0.2
Or see this region's nucleotide sequence