Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4038

Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4116 and GFF4038 are separated by 164 nucleotidesGFF4038 and GFF4039 are separated by 20 nucleotides GFF4116 - tRNA-Asp-GTC, at 238 to 314 GFF4116 GFF4038 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 479 to 1,282 GFF4038 GFF4039 - LysR family transcriptional regulator YafC, at 1,303 to 2,217 GFF4039 Position (kb) 0 1 2Strain fitness (log2 ratio) -1 0 1at 0.102 kb on + strandat 0.456 kb on + strandat 0.456 kb on + strandat 0.457 kb on - strandat 0.594 kb on - strand, within GFF4038at 0.656 kb on + strand, within GFF4038at 0.657 kb on - strand, within GFF4038at 0.702 kb on + strand, within GFF4038at 0.964 kb on + strand, within GFF4038at 1.011 kb on - strand, within GFF4038at 1.011 kb on - strand, within GFF4038at 1.069 kb on + strand, within GFF4038at 1.298 kb on + strandat 1.617 kb on + strand, within GFF4039at 1.701 kb on + strand, within GFF4039at 1.930 kb on - strand, within GFF4039at 2.000 kb on - strand, within GFF4039at 2.067 kb on + strand, within GFF4039at 2.103 kb on + strand, within GFF4039

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI
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102 + -0.7
456 + +0.9
456 + -0.5
457 - +1.0
594 - GFF4038 0.14 -0.5
656 + GFF4038 0.22 +1.0
657 - GFF4038 0.22 +0.6
702 + GFF4038 0.28 -1.0
964 + GFF4038 0.60 -0.4
1,011 - GFF4038 0.66 -0.6
1,011 - GFF4038 0.66 -0.3
1,069 + GFF4038 0.73 -1.4
1,298 + -0.8
1,617 + GFF4039 0.34 +0.4
1,701 + GFF4039 0.43 -0.2
1,930 - GFF4039 0.69 +0.6
2,000 - GFF4039 0.76 +0.0
2,067 + GFF4039 0.83 -0.4
2,103 + GFF4039 0.87 -0.4

Or see this region's nucleotide sequence