Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3622 and GFF3623 are separated by 100 nucleotides GFF3623 and GFF3624 are separated by 68 nucleotides GFF3624 and GFF3625 are separated by 58 nucleotides GFF3625 and GFF3626 are separated by 189 nucleotides
GFF3622 - Ribonuclease T (EC 3.1.13.-), at 373,755 to 374,402
GFF3622
GFF3623 - Lactoylglutathione lyase (EC 4.4.1.5), at 374,503 to 374,910
GFF3623
GFF3624 - N-ethylmaleimide reductase (EC 1.-.-.-), at 374,979 to 376,076
GFF3624
GFF3625 - Transcriptional regulator, TetR family, at 376,135 to 376,734
GFF3625
GFF3626 - FIG00553873: hypothetical protein, at 376,924 to 377,076
GFF3626
Position (kb) 374
375
376
377 Strain fitness (log2 ratio) -2
-1
0
1 at 374.414 kb on - strand at 374.715 kb on - strand, within GFF3623 at 374.715 kb on - strand, within GFF3623 at 374.892 kb on + strand at 374.893 kb on - strand at 374.896 kb on - strand at 375.097 kb on + strand, within GFF3624 at 375.098 kb on - strand, within GFF3624 at 375.166 kb on + strand, within GFF3624 at 375.266 kb on + strand, within GFF3624 at 375.267 kb on - strand, within GFF3624 at 375.267 kb on - strand, within GFF3624 at 375.275 kb on - strand, within GFF3624 at 375.454 kb on - strand, within GFF3624 at 375.701 kb on + strand, within GFF3624 at 375.764 kb on + strand, within GFF3624 at 375.944 kb on - strand, within GFF3624 at 376.092 kb on - strand at 376.245 kb on - strand, within GFF3625 at 376.535 kb on + strand, within GFF3625 at 376.671 kb on + strand, within GFF3625 at 376.746 kb on + strand at 376.793 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 374,414 - +0.5 374,715 - GFF3623 0.52 +0.0 374,715 - GFF3623 0.52 +0.8 374,892 + -0.0 374,893 - +0.3 374,896 - +0.1 375,097 + GFF3624 0.11 -0.2 375,098 - GFF3624 0.11 -0.5 375,166 + GFF3624 0.17 -0.5 375,266 + GFF3624 0.26 -2.7 375,267 - GFF3624 0.26 -0.6 375,267 - GFF3624 0.26 -0.5 375,275 - GFF3624 0.27 -1.6 375,454 - GFF3624 0.43 -0.2 375,701 + GFF3624 0.66 -0.2 375,764 + GFF3624 0.71 -2.2 375,944 - GFF3624 0.88 -0.1 376,092 - -1.2 376,245 - GFF3625 0.18 -0.1 376,535 + GFF3625 0.67 -2.4 376,671 + GFF3625 0.89 -2.2 376,746 + +0.6 376,793 + +1.1
Or see this region's nucleotide sequence