Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3492 and GFF3493 overlap by 10 nucleotides GFF3493 and GFF3494 overlap by 4 nucleotides
GFF3492 - Succinylornithine transaminase (EC 2.6.1.81), at 244,247 to 245,473
GFF3492
GFF3493 - Arginine N-succinyltransferase (EC 2.3.1.109), at 245,464 to 246,498
GFF3493
GFF3494 - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71), at 246,495 to 247,973
GFF3494
Position (kb) 245
246
247 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 244.553 kb on - strand, within GFF3492 at 244.602 kb on - strand, within GFF3492 at 244.648 kb on + strand, within GFF3492 at 244.707 kb on - strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.953 kb on - strand, within GFF3492 at 244.958 kb on + strand, within GFF3492 at 244.959 kb on - strand, within GFF3492 at 244.959 kb on - strand, within GFF3492 at 244.959 kb on - strand, within GFF3492 at 244.960 kb on + strand, within GFF3492 at 244.961 kb on - strand, within GFF3492 at 245.051 kb on + strand, within GFF3492 at 245.052 kb on - strand, within GFF3492 at 245.052 kb on - strand, within GFF3492 at 245.091 kb on - strand, within GFF3492 at 245.130 kb on + strand, within GFF3492 at 245.131 kb on - strand, within GFF3492 at 245.146 kb on + strand, within GFF3492 at 245.202 kb on + strand, within GFF3492 at 245.212 kb on + strand, within GFF3492 at 245.635 kb on + strand, within GFF3493 at 245.639 kb on + strand, within GFF3493 at 245.639 kb on + strand, within GFF3493 at 245.640 kb on - strand, within GFF3493 at 245.640 kb on - strand, within GFF3493 at 245.724 kb on + strand, within GFF3493 at 245.724 kb on + strand, within GFF3493 at 245.728 kb on + strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.771 kb on + strand, within GFF3493 at 245.771 kb on + strand, within GFF3493 at 245.772 kb on - strand, within GFF3493 at 245.776 kb on - strand, within GFF3493 at 245.792 kb on + strand, within GFF3493 at 245.875 kb on + strand, within GFF3493 at 245.899 kb on - strand, within GFF3493 at 246.097 kb on - strand, within GFF3493 at 246.100 kb on + strand, within GFF3493 at 246.209 kb on - strand, within GFF3493 at 246.209 kb on - strand, within GFF3493 at 246.250 kb on - strand, within GFF3493 at 246.508 kb on + strand at 246.575 kb on - strand at 246.575 kb on - strand at 246.719 kb on + strand, within GFF3494 at 246.822 kb on - strand, within GFF3494
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 244,553 - GFF3492 0.25 +0.3 244,602 - GFF3492 0.29 -1.1 244,648 + GFF3492 0.33 -3.9 244,707 - GFF3492 0.37 -2.3 244,795 + GFF3492 0.45 +0.0 244,795 + GFF3492 0.45 +0.1 244,795 + GFF3492 0.45 -1.4 244,953 - GFF3492 0.58 +0.8 244,958 + GFF3492 0.58 +0.2 244,959 - GFF3492 0.58 +0.5 244,959 - GFF3492 0.58 -0.7 244,959 - GFF3492 0.58 -0.2 244,960 + GFF3492 0.58 +0.4 244,961 - GFF3492 0.58 +0.9 245,051 + GFF3492 0.66 +0.0 245,052 - GFF3492 0.66 -0.9 245,052 - GFF3492 0.66 -1.4 245,091 - GFF3492 0.69 -0.5 245,130 + GFF3492 0.72 +3.4 245,131 - GFF3492 0.72 +0.8 245,146 + GFF3492 0.73 -0.2 245,202 + GFF3492 0.78 +0.6 245,212 + GFF3492 0.79 +1.8 245,635 + GFF3493 0.17 -2.4 245,639 + GFF3493 0.17 -0.6 245,639 + GFF3493 0.17 -0.8 245,640 - GFF3493 0.17 +0.3 245,640 - GFF3493 0.17 +0.6 245,724 + GFF3493 0.25 +1.8 245,724 + GFF3493 0.25 +0.4 245,728 + GFF3493 0.26 +0.9 245,729 - GFF3493 0.26 -0.6 245,729 - GFF3493 0.26 -0.8 245,729 - GFF3493 0.26 +0.6 245,729 - GFF3493 0.26 +0.4 245,771 + GFF3493 0.30 +0.2 245,771 + GFF3493 0.30 -0.3 245,772 - GFF3493 0.30 +0.5 245,776 - GFF3493 0.30 -1.1 245,792 + GFF3493 0.32 +0.2 245,875 + GFF3493 0.40 +1.4 245,899 - GFF3493 0.42 -1.6 246,097 - GFF3493 0.61 +0.8 246,100 + GFF3493 0.61 +0.0 246,209 - GFF3493 0.72 +0.8 246,209 - GFF3493 0.72 +0.9 246,250 - GFF3493 0.76 +0.2 246,508 + +1.2 246,575 - +0.0 246,575 - +0.9 246,719 + GFF3494 0.15 -0.0 246,822 - GFF3494 0.22 -0.2
Or see this region's nucleotide sequence