Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3462 and GFF3463 are separated by 68 nucleotides GFF3463 and GFF3464 overlap by 15 nucleotides GFF3464 and GFF3465 are separated by 118 nucleotides GFF3465 and GFF3466 are separated by 100 nucleotides GFF3466 and GFF3467 overlap by 21 nucleotides
GFF3462 - FIG00638802: hypothetical protein, at 215,872 to 216,123
GFF3462
GFF3463 - FIG00638667: hypothetical protein, at 216,192 to 216,503
GFF3463
GFF3464 - FIG00638254: hypothetical protein, at 216,489 to 216,836
GFF3464
GFF3465 - hypothetical protein, at 216,955 to 218,139
GFF3465
GFF3466 - Transcriptional regulator, AraC family, at 218,240 to 219,034
GFF3466
GFF3467 - Putative inner membrane protein, at 219,014 to 219,460
GFF3467
Position (kb) 216
217
218
219 Strain fitness (log2 ratio) -2
-1
0
1 at 216.150 kb on + strand at 216.223 kb on + strand at 216.281 kb on - strand, within GFF3463 at 216.539 kb on + strand, within GFF3464 at 216.553 kb on + strand, within GFF3464 at 216.727 kb on + strand, within GFF3464 at 216.727 kb on + strand, within GFF3464 at 216.953 kb on - strand at 216.953 kb on - strand at 216.956 kb on + strand at 217.040 kb on - strand at 217.223 kb on - strand, within GFF3465 at 217.429 kb on + strand, within GFF3465 at 217.548 kb on + strand, within GFF3465 at 217.548 kb on + strand, within GFF3465 at 218.068 kb on - strand at 218.467 kb on + strand, within GFF3466 at 218.762 kb on - strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.905 kb on + strand, within GFF3466 at 218.906 kb on - strand, within GFF3466 at 219.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 216,150 + -0.8 216,223 + +0.6 216,281 - GFF3463 0.29 +0.4 216,539 + GFF3464 0.14 -2.5 216,553 + GFF3464 0.18 -0.5 216,727 + GFF3464 0.68 +0.4 216,727 + GFF3464 0.68 +0.7 216,953 - -0.2 216,953 - -0.3 216,956 + +0.1 217,040 - -0.3 217,223 - GFF3465 0.23 +0.2 217,429 + GFF3465 0.40 +1.4 217,548 + GFF3465 0.50 -0.0 217,548 + GFF3465 0.50 +0.7 218,068 - -0.3 218,467 + GFF3466 0.29 -1.3 218,762 - GFF3466 0.66 -0.2 218,905 + GFF3466 0.84 +0.2 218,905 + GFF3466 0.84 -0.1 218,905 + GFF3466 0.84 -0.6 218,905 + GFF3466 0.84 +0.4 218,906 - GFF3466 0.84 +1.2 219,033 - -0.0
Or see this region's nucleotide sequence