Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3111 and GFF3112 are separated by 306 nucleotides GFF3112 and GFF3113 are separated by 279 nucleotides GFF3113 and GFF3114 are separated by 103 nucleotides
GFF3111 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 119,459 to 121,102
GFF3111
GFF3112 - Putative membrane protein, at 121,409 to 121,903
GFF3112
GFF3113 - Putative ATP-dependent RNA helicase-like protein, at 122,183 to 122,686
GFF3113
GFF3114 - FIG01045310: hypothetical protein, at 122,790 to 123,200
GFF3114
Position (kb)
121
122 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 120.526 kb on + strand, within GFF3111 at 120.527 kb on - strand, within GFF3111 at 120.546 kb on + strand, within GFF3111 at 120.547 kb on - strand, within GFF3111 at 120.547 kb on - strand, within GFF3111 at 120.547 kb on - strand, within GFF3111 at 120.767 kb on - strand, within GFF3111 at 120.938 kb on + strand at 120.939 kb on - strand at 120.974 kb on - strand at 121.014 kb on + strand at 121.015 kb on - strand at 121.017 kb on - strand at 121.078 kb on + strand at 121.180 kb on - strand at 121.182 kb on - strand at 121.182 kb on - strand at 121.183 kb on + strand at 121.183 kb on + strand at 121.184 kb on - strand at 121.291 kb on - strand at 121.497 kb on - strand, within GFF3112 at 121.692 kb on - strand, within GFF3112 at 121.876 kb on - strand at 121.880 kb on + strand at 121.881 kb on - strand at 121.938 kb on - strand at 121.939 kb on + strand at 121.939 kb on + strand at 121.984 kb on - strand at 122.169 kb on + strand at 122.184 kb on + strand at 122.345 kb on + strand, within GFF3113 at 122.346 kb on - strand, within GFF3113 at 122.476 kb on - strand, within GFF3113 at 122.476 kb on - strand, within GFF3113
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 120,526 + GFF3111 0.65 +1.8 120,527 - GFF3111 0.65 -0.3 120,546 + GFF3111 0.66 +0.8 120,547 - GFF3111 0.66 -0.1 120,547 - GFF3111 0.66 -0.3 120,547 - GFF3111 0.66 +0.7 120,767 - GFF3111 0.80 +1.4 120,938 + -1.5 120,939 - +0.4 120,974 - +0.0 121,014 + -0.7 121,015 - +0.2 121,017 - -0.2 121,078 + +0.7 121,180 - +4.0 121,182 - +0.3 121,182 - +0.1 121,183 + -0.2 121,183 + -2.3 121,184 - +1.0 121,291 - -0.5 121,497 - GFF3112 0.18 -1.0 121,692 - GFF3112 0.57 -0.3 121,876 - +1.5 121,880 + -1.0 121,881 - -1.7 121,938 - -1.2 121,939 + -0.5 121,939 + +0.4 121,984 - -0.6 122,169 + +0.4 122,184 + -1.3 122,345 + GFF3113 0.32 -0.8 122,346 - GFF3113 0.32 -1.1 122,476 - GFF3113 0.58 +0.5 122,476 - GFF3113 0.58 -0.2
Or see this region's nucleotide sequence