Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2885 and GFF2886 overlap by 1 nucleotides GFF2886 and GFF2887 overlap by 4 nucleotides
GFF2885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 8,444 to 9,511
GFF2885
GFF2886 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 9,511 to 10,773
GFF2886
GFF2887 - UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14), at 10,770 to 11,855
GFF2887
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 8.615 kb on + strand, within GFF2885 at 8.615 kb on + strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.616 kb on - strand, within GFF2885 at 8.648 kb on - strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.766 kb on + strand, within GFF2885 at 8.767 kb on - strand, within GFF2885 at 8.767 kb on - strand, within GFF2885 at 8.767 kb on - strand, within GFF2885 at 8.802 kb on + strand, within GFF2885 at 8.803 kb on - strand, within GFF2885 at 8.862 kb on + strand, within GFF2885 at 8.931 kb on + strand, within GFF2885 at 8.945 kb on - strand, within GFF2885 at 8.945 kb on - strand, within GFF2885 at 8.945 kb on - strand, within GFF2885 at 8.947 kb on + strand, within GFF2885 at 8.947 kb on + strand, within GFF2885 at 8.948 kb on - strand, within GFF2885 at 8.969 kb on - strand, within GFF2885 at 8.979 kb on + strand, within GFF2885 at 8.984 kb on - strand, within GFF2885 at 9.052 kb on - strand, within GFF2885 at 9.118 kb on + strand, within GFF2885 at 9.118 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.195 kb on + strand, within GFF2885 at 9.196 kb on - strand, within GFF2885 at 9.251 kb on + strand, within GFF2885 at 9.252 kb on - strand, within GFF2885 at 9.729 kb on + strand, within GFF2886 at 9.954 kb on + strand, within GFF2886 at 10.226 kb on - strand, within GFF2886 at 10.485 kb on - strand, within GFF2886 at 10.485 kb on - strand, within GFF2886 at 10.674 kb on + strand at 10.749 kb on + strand at 10.835 kb on - strand at 10.892 kb on + strand, within GFF2887 at 10.893 kb on - strand, within GFF2887 at 10.936 kb on - strand, within GFF2887 at 10.940 kb on + strand, within GFF2887 at 10.997 kb on + strand, within GFF2887 at 10.998 kb on - strand, within GFF2887 at 11.083 kb on + strand, within GFF2887 at 11.103 kb on + strand, within GFF2887 at 11.288 kb on + strand, within GFF2887 at 11.332 kb on - strand, within GFF2887 at 11.336 kb on + strand, within GFF2887 at 11.336 kb on + strand, within GFF2887 at 11.336 kb on + strand, within GFF2887 at 11.337 kb on - strand, within GFF2887 at 11.525 kb on - strand, within GFF2887 at 11.553 kb on + strand, within GFF2887 at 11.578 kb on + strand, within GFF2887 at 11.606 kb on + strand, within GFF2887 at 11.721 kb on - strand, within GFF2887 at 11.734 kb on + strand, within GFF2887 at 11.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 8,615 + GFF2885 0.16 -0.1 8,615 + GFF2885 0.16 +0.6 8,616 - GFF2885 0.16 +1.3 8,616 - GFF2885 0.16 +0.3 8,616 - GFF2885 0.16 -0.9 8,616 - GFF2885 0.16 +1.7 8,616 - GFF2885 0.16 +0.4 8,616 - GFF2885 0.16 -2.4 8,648 - GFF2885 0.19 +0.7 8,766 + GFF2885 0.30 -0.6 8,766 + GFF2885 0.30 +0.6 8,766 + GFF2885 0.30 +0.5 8,766 + GFF2885 0.30 -0.9 8,766 + GFF2885 0.30 -1.0 8,767 - GFF2885 0.30 +2.2 8,767 - GFF2885 0.30 +0.2 8,767 - GFF2885 0.30 -0.0 8,802 + GFF2885 0.34 +0.6 8,803 - GFF2885 0.34 +0.2 8,862 + GFF2885 0.39 -0.8 8,931 + GFF2885 0.46 -0.5 8,945 - GFF2885 0.47 +0.6 8,945 - GFF2885 0.47 +0.2 8,945 - GFF2885 0.47 +0.5 8,947 + GFF2885 0.47 +0.7 8,947 + GFF2885 0.47 -1.3 8,948 - GFF2885 0.47 +0.3 8,969 - GFF2885 0.49 +0.1 8,979 + GFF2885 0.50 +0.9 8,984 - GFF2885 0.51 -3.0 9,052 - GFF2885 0.57 -0.3 9,118 + GFF2885 0.63 -0.6 9,118 + GFF2885 0.63 +0.3 9,195 + GFF2885 0.70 -1.3 9,195 + GFF2885 0.70 -0.1 9,195 + GFF2885 0.70 +0.1 9,195 + GFF2885 0.70 -0.2 9,196 - GFF2885 0.70 -0.8 9,251 + GFF2885 0.76 -0.6 9,252 - GFF2885 0.76 -0.5 9,729 + GFF2886 0.17 +0.2 9,954 + GFF2886 0.35 +1.2 10,226 - GFF2886 0.57 -2.0 10,485 - GFF2886 0.77 +0.6 10,485 - GFF2886 0.77 -1.5 10,674 + -1.2 10,749 + -1.5 10,835 - -1.0 10,892 + GFF2887 0.11 -3.3 10,893 - GFF2887 0.11 -2.6 10,936 - GFF2887 0.15 -0.8 10,940 + GFF2887 0.16 -1.8 10,997 + GFF2887 0.21 -1.0 10,998 - GFF2887 0.21 -0.6 11,083 + GFF2887 0.29 -1.7 11,103 + GFF2887 0.31 -1.4 11,288 + GFF2887 0.48 -0.6 11,332 - GFF2887 0.52 -1.3 11,336 + GFF2887 0.52 -2.0 11,336 + GFF2887 0.52 -0.4 11,336 + GFF2887 0.52 -2.7 11,337 - GFF2887 0.52 -0.8 11,525 - GFF2887 0.70 -1.3 11,553 + GFF2887 0.72 -1.8 11,578 + GFF2887 0.74 +1.7 11,606 + GFF2887 0.77 -2.3 11,721 - GFF2887 0.88 -3.8 11,734 + GFF2887 0.89 -1.9 11,760 + +0.0
Or see this region's nucleotide sequence