Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2877 and GFF2878 are separated by 206 nucleotides GFF2878 and GFF2879 overlap by 4 nucleotides
GFF2877 - Probable transport protein YifK, at 385 to 1,770
GFF2877
GFF2878 - Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (EC 2.4.1.-), at 1,977 to 2,717
GFF2878
GFF2879 - Putative ECA polymerase, at 2,714 to 4,072
GFF2879
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1.029 kb on - strand, within GFF2877 at 1.112 kb on - strand, within GFF2877 at 1.134 kb on - strand, within GFF2877 at 1.287 kb on + strand, within GFF2877 at 1.430 kb on + strand, within GFF2877 at 1.430 kb on + strand, within GFF2877 at 1.521 kb on + strand, within GFF2877 at 1.521 kb on + strand, within GFF2877 at 1.522 kb on - strand, within GFF2877 at 1.522 kb on - strand, within GFF2877 at 1.523 kb on + strand, within GFF2877 at 1.825 kb on + strand at 1.826 kb on - strand at 1.894 kb on + strand at 1.923 kb on + strand at 1.940 kb on - strand at 1.979 kb on - strand at 2.036 kb on + strand at 2.131 kb on + strand, within GFF2878 at 2.132 kb on - strand, within GFF2878 at 2.132 kb on - strand, within GFF2878 at 2.132 kb on - strand, within GFF2878 at 2.132 kb on - strand, within GFF2878 at 2.150 kb on + strand, within GFF2878 at 2.151 kb on - strand, within GFF2878 at 2.151 kb on - strand, within GFF2878 at 2.152 kb on + strand, within GFF2878 at 2.152 kb on + strand, within GFF2878 at 2.152 kb on + strand, within GFF2878 at 2.152 kb on + strand, within GFF2878 at 2.154 kb on + strand, within GFF2878 at 2.155 kb on - strand, within GFF2878 at 2.155 kb on - strand, within GFF2878 at 2.155 kb on - strand, within GFF2878 at 2.290 kb on + strand, within GFF2878 at 2.290 kb on + strand, within GFF2878 at 2.290 kb on + strand, within GFF2878 at 2.312 kb on + strand, within GFF2878 at 2.481 kb on + strand, within GFF2878 at 2.482 kb on - strand, within GFF2878 at 2.632 kb on + strand, within GFF2878 at 2.673 kb on + strand at 2.756 kb on - strand at 3.415 kb on + strand, within GFF2879
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 1,029 - GFF2877 0.46 +0.7 1,112 - GFF2877 0.52 +1.0 1,134 - GFF2877 0.54 -0.0 1,287 + GFF2877 0.65 +0.7 1,430 + GFF2877 0.75 +0.3 1,430 + GFF2877 0.75 +0.1 1,521 + GFF2877 0.82 +0.7 1,521 + GFF2877 0.82 -2.1 1,522 - GFF2877 0.82 -0.1 1,522 - GFF2877 0.82 +0.4 1,523 + GFF2877 0.82 +0.1 1,825 + -0.5 1,826 - +0.8 1,894 + -0.9 1,923 + +0.8 1,940 - -0.4 1,979 - -1.0 2,036 + -2.7 2,131 + GFF2878 0.21 +0.4 2,132 - GFF2878 0.21 -0.7 2,132 - GFF2878 0.21 -2.4 2,132 - GFF2878 0.21 -3.5 2,132 - GFF2878 0.21 -2.8 2,150 + GFF2878 0.23 -1.3 2,151 - GFF2878 0.23 -2.5 2,151 - GFF2878 0.23 -2.1 2,152 + GFF2878 0.24 -2.7 2,152 + GFF2878 0.24 -0.8 2,152 + GFF2878 0.24 -1.3 2,152 + GFF2878 0.24 -1.9 2,154 + GFF2878 0.24 -1.3 2,155 - GFF2878 0.24 -1.6 2,155 - GFF2878 0.24 -0.0 2,155 - GFF2878 0.24 -1.6 2,290 + GFF2878 0.42 -1.8 2,290 + GFF2878 0.42 -1.5 2,290 + GFF2878 0.42 +0.3 2,312 + GFF2878 0.45 -2.1 2,481 + GFF2878 0.68 -2.1 2,482 - GFF2878 0.68 -1.1 2,632 + GFF2878 0.88 -1.8 2,673 + -2.7 2,756 - +0.0 3,415 + GFF2879 0.52 +5.6
Or see this region's nucleotide sequence