Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2632 and GFF2633 are separated by 200 nucleotides GFF2633 and GFF2634 overlap by 4 nucleotides GFF2634 and GFF2635 are separated by 265 nucleotides GFF2635 and GFF2636 are separated by 7 nucleotides
GFF2632 - ATP-dependent RNA helicase HrpA (EC 3.6.4.13), at 156,952 to 160,854
GFF2632
GFF2633 - FMN-dependent NADH-azoreductase, at 161,055 to 161,660
GFF2633
GFF2634 - Gfa-like protein, at 161,657 to 161,983
GFF2634
GFF2635 - Putative uncharacterized protein ydbL, may be related to amine metabolism, at 162,249 to 162,572
GFF2635
GFF2636 - Uncharacterized protein ynbE; probable lipoprotein STY1424, at 162,580 to 162,771
GFF2636
Position (kb) 161
162 Strain fitness (log2 ratio) -2
-1
0
1 at 160.076 kb on + strand, within GFF2632 at 160.131 kb on + strand, within GFF2632 at 160.132 kb on - strand, within GFF2632 at 160.173 kb on + strand, within GFF2632 at 160.174 kb on - strand, within GFF2632 at 160.176 kb on + strand, within GFF2632 at 160.176 kb on + strand, within GFF2632 at 160.269 kb on + strand, within GFF2632 at 160.294 kb on + strand, within GFF2632 at 160.360 kb on - strand, within GFF2632 at 160.386 kb on + strand, within GFF2632 at 160.389 kb on - strand, within GFF2632 at 160.474 kb on - strand at 160.840 kb on - strand at 160.990 kb on - strand at 160.990 kb on - strand at 161.066 kb on - strand at 161.069 kb on - strand at 161.344 kb on - strand, within GFF2633 at 161.415 kb on + strand, within GFF2633 at 161.521 kb on - strand, within GFF2633 at 161.659 kb on - strand at 161.864 kb on - strand, within GFF2634 at 161.864 kb on - strand, within GFF2634 at 161.866 kb on - strand, within GFF2634 at 162.029 kb on - strand at 162.114 kb on + strand at 162.115 kb on - strand at 162.126 kb on + strand at 162.370 kb on - strand, within GFF2635 at 162.553 kb on - strand at 162.553 kb on - strand at 162.636 kb on + strand, within GFF2636
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 160,076 + GFF2632 0.80 +0.2 160,131 + GFF2632 0.81 +0.3 160,132 - GFF2632 0.81 +0.0 160,173 + GFF2632 0.83 +0.1 160,174 - GFF2632 0.83 -0.8 160,176 + GFF2632 0.83 -0.0 160,176 + GFF2632 0.83 -1.2 160,269 + GFF2632 0.85 -0.6 160,294 + GFF2632 0.86 +0.7 160,360 - GFF2632 0.87 +0.4 160,386 + GFF2632 0.88 -0.5 160,389 - GFF2632 0.88 -2.2 160,474 - +0.3 160,840 - -0.4 160,990 - +0.8 160,990 - +1.5 161,066 - +0.6 161,069 - -0.4 161,344 - GFF2633 0.48 -0.8 161,415 + GFF2633 0.59 -0.7 161,521 - GFF2633 0.77 +0.7 161,659 - -1.4 161,864 - GFF2634 0.63 +0.2 161,864 - GFF2634 0.63 +0.1 161,866 - GFF2634 0.64 +1.2 162,029 - +1.3 162,114 + -0.2 162,115 - +0.2 162,126 + +1.1 162,370 - GFF2635 0.37 -0.1 162,553 - -0.3 162,553 - +1.1 162,636 + GFF2636 0.29 +0.6
Or see this region's nucleotide sequence