Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2330 and GFF2331 are separated by 57 nucleotides GFF2331 and GFF2332 are separated by 258 nucleotides
GFF2330 - Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7), at 118,507 to 120,687
GFF2330
GFF2331 - Putative periplasmic protein, at 120,745 to 121,368
GFF2331
GFF2332 - Glycerol dehydrogenase (EC 1.1.1.6), at 121,627 to 122,730
GFF2332
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-1
0
1
2 at 119.834 kb on - strand, within GFF2330 at 119.895 kb on + strand, within GFF2330 at 119.895 kb on + strand, within GFF2330 at 119.953 kb on - strand, within GFF2330 at 120.041 kb on + strand, within GFF2330 at 120.045 kb on - strand, within GFF2330 at 120.090 kb on + strand, within GFF2330 at 120.090 kb on + strand, within GFF2330 at 120.091 kb on - strand, within GFF2330 at 120.156 kb on - strand, within GFF2330 at 120.297 kb on + strand, within GFF2330 at 120.312 kb on - strand, within GFF2330 at 120.439 kb on - strand, within GFF2330 at 120.458 kb on - strand, within GFF2330 at 120.572 kb on + strand at 120.596 kb on - strand at 120.717 kb on - strand at 120.758 kb on + strand at 120.790 kb on + strand at 120.791 kb on - strand at 120.793 kb on + strand at 120.820 kb on + strand, within GFF2331 at 120.820 kb on + strand, within GFF2331 at 120.826 kb on - strand, within GFF2331 at 120.880 kb on - strand, within GFF2331 at 120.916 kb on - strand, within GFF2331 at 120.916 kb on - strand, within GFF2331 at 120.916 kb on - strand, within GFF2331 at 120.936 kb on + strand, within GFF2331 at 120.939 kb on - strand, within GFF2331 at 120.942 kb on + strand, within GFF2331 at 121.193 kb on + strand, within GFF2331 at 121.210 kb on + strand, within GFF2331 at 121.211 kb on - strand, within GFF2331 at 121.218 kb on + strand, within GFF2331 at 121.219 kb on - strand, within GFF2331 at 121.219 kb on - strand, within GFF2331 at 121.219 kb on - strand, within GFF2331 at 121.223 kb on - strand, within GFF2331 at 121.258 kb on - strand, within GFF2331 at 121.348 kb on - strand at 121.536 kb on + strand at 121.537 kb on - strand at 121.628 kb on + strand at 121.629 kb on - strand at 121.720 kb on - strand at 121.726 kb on - strand at 121.788 kb on + strand, within GFF2332 at 121.789 kb on - strand, within GFF2332 at 121.923 kb on - strand, within GFF2332 at 121.926 kb on - strand, within GFF2332 at 121.926 kb on - strand, within GFF2332 at 122.109 kb on + strand, within GFF2332 at 122.111 kb on + strand, within GFF2332 at 122.112 kb on - strand, within GFF2332 at 122.117 kb on - strand, within GFF2332 at 122.145 kb on + strand, within GFF2332 at 122.340 kb on - strand, within GFF2332 at 122.344 kb on - strand, within GFF2332
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 119,834 - GFF2330 0.61 +2.7 119,895 + GFF2330 0.64 -0.7 119,895 + GFF2330 0.64 +0.2 119,953 - GFF2330 0.66 -0.6 120,041 + GFF2330 0.70 +0.7 120,045 - GFF2330 0.71 +0.1 120,090 + GFF2330 0.73 +0.7 120,090 + GFF2330 0.73 +0.5 120,091 - GFF2330 0.73 +0.1 120,156 - GFF2330 0.76 -1.3 120,297 + GFF2330 0.82 -0.4 120,312 - GFF2330 0.83 +0.5 120,439 - GFF2330 0.89 -1.1 120,458 - GFF2330 0.89 +0.9 120,572 + +0.8 120,596 - -0.0 120,717 - +0.8 120,758 + -0.4 120,790 + -0.0 120,791 - -0.3 120,793 + +0.5 120,820 + GFF2331 0.12 -0.2 120,820 + GFF2331 0.12 -0.4 120,826 - GFF2331 0.13 -0.5 120,880 - GFF2331 0.22 -0.4 120,916 - GFF2331 0.27 +0.1 120,916 - GFF2331 0.27 +0.0 120,916 - GFF2331 0.27 -0.5 120,936 + GFF2331 0.31 -0.0 120,939 - GFF2331 0.31 -0.4 120,942 + GFF2331 0.32 +1.4 121,193 + GFF2331 0.72 -0.1 121,210 + GFF2331 0.75 +0.1 121,211 - GFF2331 0.75 +0.2 121,218 + GFF2331 0.76 +0.1 121,219 - GFF2331 0.76 +0.3 121,219 - GFF2331 0.76 -0.1 121,219 - GFF2331 0.76 +0.4 121,223 - GFF2331 0.77 +0.4 121,258 - GFF2331 0.82 +0.6 121,348 - -0.5 121,536 + -0.0 121,537 - -0.1 121,628 + -0.1 121,629 - -1.4 121,720 - +1.6 121,726 - -0.1 121,788 + GFF2332 0.15 -0.3 121,789 - GFF2332 0.15 -0.3 121,923 - GFF2332 0.27 +0.1 121,926 - GFF2332 0.27 -0.4 121,926 - GFF2332 0.27 +0.0 122,109 + GFF2332 0.44 +0.4 122,111 + GFF2332 0.44 +0.3 122,112 - GFF2332 0.44 +0.0 122,117 - GFF2332 0.44 -1.2 122,145 + GFF2332 0.47 -0.1 122,340 - GFF2332 0.65 -0.9 122,344 - GFF2332 0.65 +0.1
Or see this region's nucleotide sequence