Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2266 and GFF2267 are separated by 152 nucleotides GFF2267 and GFF2268 overlap by 4 nucleotides
GFF2266 - L-rhamnose operon regulatory protein RhaS, at 57,989 to 58,825
GFF2266
GFF2267 - L-rhamnose operon transcriptional activator RhaR, at 58,978 to 59,826
GFF2267
GFF2268 - L-rhamnose-proton symporter, at 59,823 to 60,857
GFF2268
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-1
0
1
2 at 58.004 kb on - strand at 58.135 kb on - strand, within GFF2266 at 58.135 kb on - strand, within GFF2266 at 58.151 kb on + strand, within GFF2266 at 58.153 kb on + strand, within GFF2266 at 58.203 kb on + strand, within GFF2266 at 58.203 kb on + strand, within GFF2266 at 58.213 kb on + strand, within GFF2266 at 58.224 kb on + strand, within GFF2266 at 58.224 kb on + strand, within GFF2266 at 58.225 kb on - strand, within GFF2266 at 58.239 kb on + strand, within GFF2266 at 58.313 kb on + strand, within GFF2266 at 58.317 kb on - strand, within GFF2266 at 58.439 kb on - strand, within GFF2266 at 58.446 kb on + strand, within GFF2266 at 58.700 kb on - strand, within GFF2266 at 58.857 kb on - strand at 58.865 kb on - strand at 58.868 kb on + strand at 58.868 kb on + strand at 58.901 kb on + strand at 58.902 kb on - strand at 58.935 kb on - strand at 58.935 kb on - strand at 58.940 kb on + strand at 59.079 kb on + strand, within GFF2267 at 59.080 kb on - strand, within GFF2267 at 59.080 kb on - strand, within GFF2267 at 59.126 kb on + strand, within GFF2267 at 59.137 kb on - strand, within GFF2267 at 59.203 kb on + strand, within GFF2267 at 59.204 kb on - strand, within GFF2267 at 59.204 kb on - strand, within GFF2267 at 59.206 kb on - strand, within GFF2267 at 59.206 kb on - strand, within GFF2267 at 59.523 kb on + strand, within GFF2267 at 59.523 kb on + strand, within GFF2267 at 59.665 kb on - strand, within GFF2267 at 59.868 kb on + strand at 59.886 kb on + strand at 59.889 kb on + strand at 59.890 kb on - strand at 59.890 kb on - strand at 59.929 kb on - strand, within GFF2268 at 59.962 kb on + strand, within GFF2268 at 59.962 kb on + strand, within GFF2268 at 59.963 kb on - strand, within GFF2268 at 59.963 kb on - strand, within GFF2268 at 59.993 kb on - strand, within GFF2268 at 59.993 kb on - strand, within GFF2268 at 59.998 kb on + strand, within GFF2268 at 59.998 kb on + strand, within GFF2268 at 60.031 kb on + strand, within GFF2268 at 60.226 kb on + strand, within GFF2268 at 60.227 kb on - strand, within GFF2268 at 60.227 kb on - strand, within GFF2268 at 60.475 kb on - strand, within GFF2268 at 60.475 kb on - strand, within GFF2268 at 60.488 kb on + strand, within GFF2268 at 60.501 kb on - strand, within GFF2268 at 60.501 kb on - strand, within GFF2268 at 60.531 kb on - strand, within GFF2268 at 60.589 kb on + strand, within GFF2268 at 60.594 kb on - strand, within GFF2268 at 60.594 kb on - strand, within GFF2268 at 60.594 kb on - strand, within GFF2268 at 60.667 kb on + strand, within GFF2268 at 60.790 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 58,004 - -0.4 58,135 - GFF2266 0.17 +1.6 58,135 - GFF2266 0.17 -0.1 58,151 + GFF2266 0.19 -0.1 58,153 + GFF2266 0.20 +1.0 58,203 + GFF2266 0.26 -0.5 58,203 + GFF2266 0.26 -0.8 58,213 + GFF2266 0.27 +0.1 58,224 + GFF2266 0.28 -0.3 58,224 + GFF2266 0.28 -0.3 58,225 - GFF2266 0.28 -1.3 58,239 + GFF2266 0.30 -0.0 58,313 + GFF2266 0.39 -0.2 58,317 - GFF2266 0.39 -1.6 58,439 - GFF2266 0.54 -0.4 58,446 + GFF2266 0.55 -0.3 58,700 - GFF2266 0.85 -0.3 58,857 - -0.6 58,865 - +0.7 58,868 + -0.9 58,868 + +0.0 58,901 + +2.1 58,902 - -1.4 58,935 - +0.3 58,935 - +0.2 58,940 + +0.1 59,079 + GFF2267 0.12 +1.6 59,080 - GFF2267 0.12 -0.0 59,080 - GFF2267 0.12 -0.3 59,126 + GFF2267 0.17 +0.3 59,137 - GFF2267 0.19 -0.2 59,203 + GFF2267 0.27 -0.2 59,204 - GFF2267 0.27 -0.7 59,204 - GFF2267 0.27 -0.5 59,206 - GFF2267 0.27 -0.3 59,206 - GFF2267 0.27 +0.3 59,523 + GFF2267 0.64 -0.5 59,523 + GFF2267 0.64 -0.6 59,665 - GFF2267 0.81 +0.2 59,868 + +0.1 59,886 + +0.2 59,889 + -0.1 59,890 - -0.0 59,890 - -0.2 59,929 - GFF2268 0.10 -0.5 59,962 + GFF2268 0.13 -0.9 59,962 + GFF2268 0.13 +1.8 59,963 - GFF2268 0.14 +0.6 59,963 - GFF2268 0.14 -0.9 59,993 - GFF2268 0.16 -0.0 59,993 - GFF2268 0.16 +0.2 59,998 + GFF2268 0.17 -0.8 59,998 + GFF2268 0.17 +0.4 60,031 + GFF2268 0.20 +0.9 60,226 + GFF2268 0.39 +0.3 60,227 - GFF2268 0.39 -0.6 60,227 - GFF2268 0.39 -0.1 60,475 - GFF2268 0.63 -0.1 60,475 - GFF2268 0.63 +0.1 60,488 + GFF2268 0.64 -0.0 60,501 - GFF2268 0.66 -1.1 60,501 - GFF2268 0.66 -1.4 60,531 - GFF2268 0.68 +1.1 60,589 + GFF2268 0.74 +0.1 60,594 - GFF2268 0.74 -1.5 60,594 - GFF2268 0.74 -0.2 60,594 - GFF2268 0.74 +0.5 60,667 + GFF2268 0.82 -0.9 60,790 + +0.3
Or see this region's nucleotide sequence