Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2260 and GFF2261 are separated by 88 nucleotides GFF2261 and GFF2262 overlap by 4 nucleotides GFF2262 and GFF2263 are separated by 126 nucleotides
GFF2260 - Transcriptional regulator, at 51,783 to 52,331
GFF2260
GFF2261 - L-rhamnose mutarotase, at 52,420 to 52,734
GFF2261
GFF2262 - Lactaldehyde dehydrogenase involved in fucose or rhamnose utilization (EC 1.2.1.22), at 52,731 to 53,879
GFF2262
GFF2263 - Rhamnulose-1-phosphate aldolase (EC 4.1.2.19), at 54,006 to 54,833
GFF2263
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 51.782 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.888 kb on - strand, within GFF2260 at 52.070 kb on - strand, within GFF2260 at 52.104 kb on - strand, within GFF2260 at 52.107 kb on + strand, within GFF2260 at 52.107 kb on + strand, within GFF2260 at 52.176 kb on - strand, within GFF2260 at 52.178 kb on + strand, within GFF2260 at 52.178 kb on + strand, within GFF2260 at 52.178 kb on + strand, within GFF2260 at 52.274 kb on + strand, within GFF2260 at 52.274 kb on + strand, within GFF2260 at 52.275 kb on - strand, within GFF2260 at 52.422 kb on - strand at 52.430 kb on + strand at 52.444 kb on + strand at 52.485 kb on + strand, within GFF2261 at 52.497 kb on + strand, within GFF2261 at 52.497 kb on + strand, within GFF2261 at 52.498 kb on - strand, within GFF2261 at 52.498 kb on - strand, within GFF2261 at 52.668 kb on + strand, within GFF2261 at 52.705 kb on + strand at 52.734 kb on + strand at 52.735 kb on - strand at 52.787 kb on + strand at 52.898 kb on + strand, within GFF2262 at 52.898 kb on + strand, within GFF2262 at 52.899 kb on - strand, within GFF2262 at 52.944 kb on - strand, within GFF2262 at 52.956 kb on + strand, within GFF2262 at 53.178 kb on - strand, within GFF2262 at 53.217 kb on + strand, within GFF2262 at 53.275 kb on + strand, within GFF2262 at 53.469 kb on - strand, within GFF2262 at 53.469 kb on - strand, within GFF2262 at 53.469 kb on - strand, within GFF2262 at 53.472 kb on - strand, within GFF2262 at 53.475 kb on - strand, within GFF2262 at 53.870 kb on + strand at 53.871 kb on - strand at 53.960 kb on + strand at 54.004 kb on + strand at 54.005 kb on - strand at 54.123 kb on + strand, within GFF2263 at 54.124 kb on - strand, within GFF2263 at 54.147 kb on - strand, within GFF2263 at 54.176 kb on + strand, within GFF2263 at 54.177 kb on - strand, within GFF2263 at 54.379 kb on + strand, within GFF2263 at 54.380 kb on - strand, within GFF2263 at 54.399 kb on - strand, within GFF2263 at 54.417 kb on - strand, within GFF2263 at 54.749 kb on + strand, within GFF2263 at 54.750 kb on - strand, within GFF2263 at 54.822 kb on - strand at 54.849 kb on + strand at 54.879 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 51,782 + -0.7 51,794 + +0.4 51,794 + +1.6 51,794 + -0.9 51,794 + -0.3 51,794 + -1.7 51,888 - GFF2260 0.19 +0.3 52,070 - GFF2260 0.52 +0.8 52,104 - GFF2260 0.58 +0.5 52,107 + GFF2260 0.59 +0.9 52,107 + GFF2260 0.59 +0.4 52,176 - GFF2260 0.72 -0.4 52,178 + GFF2260 0.72 -0.7 52,178 + GFF2260 0.72 +0.3 52,178 + GFF2260 0.72 -0.6 52,274 + GFF2260 0.89 -0.1 52,274 + GFF2260 0.89 +0.7 52,275 - GFF2260 0.90 +0.8 52,422 - -0.1 52,430 + +0.6 52,444 + +1.3 52,485 + GFF2261 0.21 +0.3 52,497 + GFF2261 0.24 -0.9 52,497 + GFF2261 0.24 +0.8 52,498 - GFF2261 0.25 -0.5 52,498 - GFF2261 0.25 +0.5 52,668 + GFF2261 0.79 -1.7 52,705 + +0.3 52,734 + -0.6 52,735 - +0.3 52,787 + +0.3 52,898 + GFF2262 0.15 +0.6 52,898 + GFF2262 0.15 -1.4 52,899 - GFF2262 0.15 -0.5 52,944 - GFF2262 0.19 +1.5 52,956 + GFF2262 0.20 +1.3 53,178 - GFF2262 0.39 +0.6 53,217 + GFF2262 0.42 +1.2 53,275 + GFF2262 0.47 -2.5 53,469 - GFF2262 0.64 -0.0 53,469 - GFF2262 0.64 -0.2 53,469 - GFF2262 0.64 -0.5 53,472 - GFF2262 0.64 +0.1 53,475 - GFF2262 0.65 +0.3 53,870 + +0.2 53,871 - -0.6 53,960 + -1.4 54,004 + +0.6 54,005 - -0.4 54,123 + GFF2263 0.14 -0.4 54,124 - GFF2263 0.14 +0.8 54,147 - GFF2263 0.17 +0.9 54,176 + GFF2263 0.21 -0.0 54,177 - GFF2263 0.21 -0.5 54,379 + GFF2263 0.45 +0.8 54,380 - GFF2263 0.45 +2.0 54,399 - GFF2263 0.47 -0.0 54,417 - GFF2263 0.50 -0.1 54,749 + GFF2263 0.90 +0.7 54,750 - GFF2263 0.90 +0.2 54,822 - +0.2 54,849 + -0.0 54,879 - -1.8
Or see this region's nucleotide sequence