Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2195 and GFF2196 are separated by 19 nucleotides GFF2196 and GFF2197 are separated by 69 nucleotides GFF2197 and GFF2198 are separated by 31 nucleotides
GFF2195 - Transketolase (EC 2.2.1.1), at 45,823 to 47,823
GFF2195
GFF2196 - Transaldolase (EC 2.2.1.2), at 47,843 to 48,793
GFF2196
GFF2197 - FIG01047668: hypothetical protein, at 48,863 to 49,033
GFF2197
GFF2198 - NADP-dependent malic enzyme (EC 1.1.1.40), at 49,065 to 51,344
GFF2198
Position (kb)
47
48
49 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 47.189 kb on - strand, within GFF2195 at 47.314 kb on - strand, within GFF2195 at 47.378 kb on + strand, within GFF2195 at 47.378 kb on + strand, within GFF2195 at 47.378 kb on + strand, within GFF2195 at 47.384 kb on + strand, within GFF2195 at 47.384 kb on + strand, within GFF2195 at 47.433 kb on + strand, within GFF2195 at 47.610 kb on + strand, within GFF2195 at 47.613 kb on - strand, within GFF2195 at 47.663 kb on + strand at 47.686 kb on - strand at 47.686 kb on - strand at 47.708 kb on - strand at 47.823 kb on + strand at 47.823 kb on + strand at 47.846 kb on + strand at 48.067 kb on + strand, within GFF2196 at 48.214 kb on - strand, within GFF2196 at 48.230 kb on - strand, within GFF2196 at 48.344 kb on - strand, within GFF2196 at 48.559 kb on + strand, within GFF2196 at 48.683 kb on + strand, within GFF2196 at 48.683 kb on + strand, within GFF2196 at 48.864 kb on + strand at 48.864 kb on + strand at 48.864 kb on + strand at 48.865 kb on - strand at 48.869 kb on - strand at 48.908 kb on + strand, within GFF2197 at 48.909 kb on - strand, within GFF2197 at 48.916 kb on + strand, within GFF2197 at 48.931 kb on - strand, within GFF2197 at 49.006 kb on + strand, within GFF2197 at 49.242 kb on + strand at 49.243 kb on - strand at 49.670 kb on + strand, within GFF2198 at 49.737 kb on - strand, within GFF2198 at 49.740 kb on + strand, within GFF2198
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 47,189 - GFF2195 0.68 -0.8 47,314 - GFF2195 0.75 +0.3 47,378 + GFF2195 0.78 -0.8 47,378 + GFF2195 0.78 +0.7 47,378 + GFF2195 0.78 -1.6 47,384 + GFF2195 0.78 +0.8 47,384 + GFF2195 0.78 -0.5 47,433 + GFF2195 0.80 +0.6 47,610 + GFF2195 0.89 -0.6 47,613 - GFF2195 0.89 +0.0 47,663 + -1.9 47,686 - +0.3 47,686 - +0.3 47,708 - +1.3 47,823 + +0.4 47,823 + +1.4 47,846 + +0.1 48,067 + GFF2196 0.24 +1.0 48,214 - GFF2196 0.39 -1.9 48,230 - GFF2196 0.41 -2.9 48,344 - GFF2196 0.53 -0.1 48,559 + GFF2196 0.75 -2.1 48,683 + GFF2196 0.88 -1.9 48,683 + GFF2196 0.88 -1.6 48,864 + +0.2 48,864 + -0.7 48,864 + +0.7 48,865 - +0.1 48,869 - -0.3 48,908 + GFF2197 0.26 -0.6 48,909 - GFF2197 0.27 +0.4 48,916 + GFF2197 0.31 +0.5 48,931 - GFF2197 0.40 -0.6 49,006 + GFF2197 0.84 +0.2 49,242 + -0.3 49,243 - -0.3 49,670 + GFF2198 0.27 +1.2 49,737 - GFF2198 0.29 -0.3 49,740 + GFF2198 0.30 -0.2
Or see this region's nucleotide sequence