Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1996

Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1995 and GFF1996 are separated by 322 nucleotidesGFF1996 and GFF1997 overlap by 8 nucleotidesGFF1997 and GFF1998 are separated by 10 nucleotides GFF1995 - Translation elongation factor P Lys34:lysine transferase, at 9,402 to 10,379 GFF1995 GFF1996 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 10,702 to 12,492 GFF1996 GFF1997 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 12,485 to 13,219 GFF1997 GFF1998 - Fumarate reductase subunit C, at 13,230 to 13,625 GFF1998 Position (kb) 10 11 12 13Strain fitness (log2 ratio) -3 -2 -1 0 1at 10.011 kb on - strand, within GFF1995at 10.416 kb on + strandat 10.504 kb on - strandat 10.594 kb on + strandat 10.595 kb on - strandat 10.595 kb on - strandat 10.608 kb on + strandat 10.680 kb on + strandat 10.681 kb on - strandat 10.685 kb on + strandat 10.686 kb on - strandat 10.733 kb on + strandat 10.733 kb on + strandat 10.733 kb on + strandat 10.895 kb on - strand, within GFF1996at 11.106 kb on + strand, within GFF1996at 11.115 kb on + strand, within GFF1996at 11.116 kb on - strand, within GFF1996at 11.182 kb on - strand, within GFF1996at 11.305 kb on + strand, within GFF1996at 11.472 kb on + strand, within GFF1996at 11.548 kb on - strand, within GFF1996at 11.716 kb on + strand, within GFF1996at 11.770 kb on + strand, within GFF1996at 11.771 kb on - strand, within GFF1996at 11.771 kb on - strand, within GFF1996at 12.100 kb on + strand, within GFF1996at 12.100 kb on + strand, within GFF1996at 12.136 kb on - strand, within GFF1996at 12.170 kb on + strand, within GFF1996at 12.171 kb on - strand, within GFF1996at 12.214 kb on + strand, within GFF1996at 12.214 kb on + strand, within GFF1996at 12.215 kb on - strand, within GFF1996at 12.217 kb on - strand, within GFF1996at 12.255 kb on + strand, within GFF1996at 12.255 kb on + strand, within GFF1996at 12.469 kb on - strandat 12.611 kb on + strand, within GFF1997at 12.611 kb on + strand, within GFF1997at 12.738 kb on - strand, within GFF1997at 12.842 kb on + strand, within GFF1997at 12.842 kb on + strand, within GFF1997at 12.847 kb on + strand, within GFF1997at 12.847 kb on + strand, within GFF1997at 12.951 kb on - strand, within GFF1997at 13.238 kb on + strandat 13.251 kb on + strandat 13.251 kb on + strandat 13.252 kb on - strandat 13.252 kb on - strandat 13.305 kb on + strand, within GFF1998at 13.306 kb on - strand, within GFF1998at 13.307 kb on + strand, within GFF1998at 13.308 kb on - strand, within GFF1998

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI
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10,011 - GFF1995 0.62 -0.8
10,416 + +1.4
10,504 - -0.9
10,594 + +0.1
10,595 - -0.0
10,595 - +0.5
10,608 + +0.2
10,680 + +0.1
10,681 - +0.4
10,685 + -1.0
10,686 - +0.1
10,733 + -1.2
10,733 + -0.4
10,733 + -0.8
10,895 - GFF1996 0.11 +0.8
11,106 + GFF1996 0.23 +1.0
11,115 + GFF1996 0.23 +0.4
11,116 - GFF1996 0.23 -0.8
11,182 - GFF1996 0.27 -0.1
11,305 + GFF1996 0.34 -0.3
11,472 + GFF1996 0.43 +0.1
11,548 - GFF1996 0.47 +1.0
11,716 + GFF1996 0.57 +0.6
11,770 + GFF1996 0.60 -0.7
11,771 - GFF1996 0.60 -0.1
11,771 - GFF1996 0.60 +0.3
12,100 + GFF1996 0.78 -0.6
12,100 + GFF1996 0.78 -0.5
12,136 - GFF1996 0.80 +0.0
12,170 + GFF1996 0.82 +0.3
12,171 - GFF1996 0.82 -0.4
12,214 + GFF1996 0.84 -1.4
12,214 + GFF1996 0.84 -0.2
12,215 - GFF1996 0.84 +0.4
12,217 - GFF1996 0.85 -0.7
12,255 + GFF1996 0.87 +0.3
12,255 + GFF1996 0.87 +0.4
12,469 - +0.2
12,611 + GFF1997 0.17 +0.1
12,611 + GFF1997 0.17 -0.6
12,738 - GFF1997 0.34 -0.0
12,842 + GFF1997 0.49 -1.2
12,842 + GFF1997 0.49 -0.5
12,847 + GFF1997 0.49 +0.4
12,847 + GFF1997 0.49 -0.2
12,951 - GFF1997 0.63 +0.4
13,238 + -0.2
13,251 + -0.0
13,251 + +0.2
13,252 - -0.3
13,252 - +0.3
13,305 + GFF1998 0.19 +0.2
13,306 - GFF1998 0.19 +0.4
13,307 + GFF1998 0.19 -3.4
13,308 - GFF1998 0.20 +0.3

Or see this region's nucleotide sequence